Opisthorchiasis from Imported Raw Fish

Yossepowitch, Orit; Gotesman, Tamar; Assous, Mark; Marva, Esther; Zimlichman, Reuven; Dan, Michael

doi:10.3201/eid1012.040410

Cited by 92 publications

(46 citation statements)

References 11 publications

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“…The disease is endemic in many parts of the Far East, Southeast Asia and eastern Europe and can be spread over large distances via infected fish (Yossepowitch et al, 2004). The main public health problem in Southeast Asia arises from infection with trematodes belonging to the family Opisthorchiidae, such as Opisthorchis viverrini, O. felineus and Clonorchis sinensis.…”

Section: Introductionmentioning

confidence: 99%

Epidemiology of Opisthorchis viverrini in a rural district of southern Lao PDR

Sayasone

Odermatt

Phoumindr

et al. 2007

Transactions of the Royal Society of Tropical Medicine and Hygi

131

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a v a i l a b l e a t w w w . s c i e n c e d i r e c t . c o m j o u r n a l h o m e p a g e : w w w . e l s e v i e r h e a l t h . c o m / j o u r n a l s / t r s t Summary In Lao PDR, detailed investigations on Opisthorchis viverrini are scarce. The objective was to document epidemiological features of O. viverrini infections in a highly endemic district. A random sample was selected (13 villages, 15 households/village, all household members aged >6 months). Clinical examinations, short interviews and stool examinations (Kato-Katz technique) were performed. Fish samples were dissected for the presence of metacercaria. In total, 814 persons were enrolled (median age 16 years, 51.5% women). The prevalence was 58.5%. Infection rates increased with age (from 20.0 to 85.5%; P < 0.001). Intensity of infection and the habit of consuming insufficiently cooked fish also increased with age. Of the study participants, 75.2% reported cooking fish insufficiently. Of the 23 different species of cyprinoid fish consumed in the study villages, 20 species were infected. At the village level, the prevalence of raw fish consumption was strongly associated with the infection status of O. viverrini (r = 0.76, P = 0.003). At individual level, age, the consumption of insufficiently cooked fish, and the absence of sanitation were strongly associated. The disease associated with O. viverrini infection needs to be assessed in order to develop and conduct adequate interventions. Epidemiology of

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Section: Introductionmentioning

confidence: 99%

Epidemiology of Opisthorchis viverrini in a rural district of southern Lao PDR

Sayasone

Odermatt

Phoumindr

et al. 2007

Transactions of the Royal Society of Tropical Medicine and Hygi

131

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“…1 The estimated prevalence ranges from 9.4% to 79% and approximately 9 million persons are infected. 2,3 In developed countries, the disease has become more prevalent with the influx of immigrants from Asia. [4][5][6] Humans are infected by eating raw or semi-cooked cyprinoid fishes harboring the O. viverrini infective stage called metacercariae.…”

Section: Introductionmentioning

confidence: 99%

Rapid Molecular Detection of Opisthorchis viverrini in Human Fecal Samples by Real-Time Polymerase Chain Reaction

Intapan

Thanchomnang²,

Lulitanond³

et al. 2009

The American Journal of Tropical Medicine and Hygiene

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Abstract. Real-time fluorescence resonance energy transfer (FRET) polymerase chain reaction (PCR) supplemented with melting curve analysis is a highly sensitive and fast method offering a high throughput. We report the development of a real-time FRET PCR for molecular detection of Opisthorchis viverrini in human fecal samples. The diagnostic sensitivity, specificity, accuracy, and positive and negative predictive values of this method were 97.5%, 100%, 98.9%, 100%, and 98.2%, respectively. The sensitivity was not significantly different from that of the quantified formalin-ethyl acetate concentration technique, the gold standard ( P > 0.05). The procedure has potential for diagnosis of human opisthorchiasis in disease-endemic areas, for large epidemiologic investigations involving at risk populations, and monitoring eradication programs of the liver fluke, which causes hepatobiliary diseases and induces cholangiocarcinoma.

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“…In Europe, O. felineus in humans is endemic in Byelorussia, Russia and Ukraine (Keiser and Utzinger, 2005), with prevalence ranging from 5% to 40% (Zavoikin et al, 1989;Nesterenko et al, 1990) and sporadically recorded in Germany, Greece, Poland and Baltic states (Yossepowitch et al, 2004;Armignacco et al, 2008). In Italy, O. felineus was first described at the end of the 19th century (Rivolta, 1884;Perroncito, 1901) from cats and dogs.…”

Section: Introductionmentioning

confidence: 99%

“…The muskrat (Ondatra zibethicus) was found positive in former USSR (Shuteev, 1977) and Germany (Pauly et al, 2003). The human infection follows the consumption of raw, undercooked, salted, cold smoked or marinated fish (Yossepowitch et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…doi: 10.1016/j.vetpar.2010.11.042 wide suffer from opisthorchiasis (WHO, 1995;Schuster, 2010) and their number is increasing in some areas (Keiser and Utzinger, 2005;Marcos et al, 2008). In particular, O. felineus affects 1.6 million people (Yossepowitch et al, 2004), causing abdominal pain, fever, diffuse myalgia, diarrhoea, cholangitis, pancreatitis and possibly, in long lasting chronic infections, cholangiocarcinoma (Sripa, 2003). Adult flukes, living in biliary and pancreatic ducts of the definitive host, deposit eggs that reach the environment through host feces.…”

Section: Introductionmentioning

confidence: 99%

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Investigation on Opisthorchis felineus occurrence and life cycle in Italy

Liberato

Scaramozzino

Brozzi

et al. 2011

Veterinary Parasitology

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a b s t r a c tOpisthorchiasis is a fish borne parasitic infection caused by helminths of the genus Opisthorchis (Digenea, Opisthorchiidae), affecting humans and other fish-eating mammals. Despite Opisthorchis felineus was first described in Italy in 1884, no cases of human opisthorchiasis were reported in this country until 2004; from then on, 4 outbreaks due to this species have been recorded in Central Italy. Following the more relevant of these outbreaks, involving 34 people in August 2007, snails, fishes and fecal samples collected from the Bolsena and Bracciano lakes (Central Italy) were analyzed in order to define the cycle of O. felineus in the area and investigate its prevalence in the different hosts. Pools of 20-40 snails each (4983 specimens altogether) of the genus Bithynia were analyzed by PCR for parasite DNA detection. Eight hundred and ninety-four fish belonging to 12 species were collected from the two lakes and tested for metacercariae both by muscle compression and digestion techniques. Eighty-seven fecal samples of 5 putative definitive host species were collected very close to the two lakes and tested for parasite eggs detection by formalin-ethyl acetate concentration technique. Identification at the species level of metacercariae and eggs, respectively, from fish and stool was confirmed by PCR analysis and sequencing. O. felineus DNA was detected in 0.08% (overall minimum infection rate) of snails of the genus Bithynia from the two lakes. The tench, Tinca tinca, was the only fish found infested in both lakes (prevalence 88.5%). O. felineus eggs were found only in cat feces (prevalence 46.4%). The tench represents the only threat for the human consumption in the study area while Coregonus sp., the most economically important species for the local fishery and frequently consumed raw marinated, resulted to be not infected. The high prevalence recorded both in fish and in definitive host suggests a widespread and massive presence of the parasite in the area. Further studies are needed to better investigate the possible role of some cyprinids species as intermediate hosts, in order to check their safety for human consumption.

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Opisthorchiasis from Imported Raw Fish

Abstract: Acute liver fluke infection results from eating raw fish illegally imported from Siberia.

Cited by 92 publications

References 11 publications

Epidemiology of Opisthorchis viverrini in a rural district of southern Lao PDR

Epidemiology of Opisthorchis viverrini in a rural district of southern Lao PDR

Rapid Molecular Detection of Opisthorchis viverrini in Human Fecal Samples by Real-Time Polymerase Chain Reaction

Investigation on Opisthorchis felineus occurrence and life cycle in Italy

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