Opioid Neurobiology, Neurogenetics and Neuropharmacology in Zebrafish

Bao, Wandong; Volgin, Andrey D.; Alpyshov, Erik T.; Friend, Ashton J.; Strekalova, Tatyana; Abreu, Murilo S. de; Collins, Christopher; Amstislavskaya, Tamara G.; Demin, Konstantin A.; Kalueff, Allan V.

doi:10.1016/j.neuroscience.2019.01.045

Cited by 37 publications

(31 citation statements)

References 185 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Zebrafish (Danio rerio) are one of the species most used in research in fields ranging from developmental biology to neurobehaviour 19,20 and ecotoxicology 21,22 . Zebrafish can also be conveniently used for studying the effects of thermal variation given its wide thermal tolerance (6.7 to 41.7 °C) 23,24 .…”

mentioning

confidence: 99%

Increase in environmental temperature affects exploratory behaviour, anxiety and social preference in Danio rerio

Angiulli¹,

Pagliara²,

Cioni³

et al. 2020

Sci Rep

View full text Add to dashboard Cite

The aim of this work is to investigate the effect of a temperature increase on the behaviour of adult zebrafish (Danio rerio) maintained for 21 days at 34 °C (treatment) and 26 °C (control). The temperatures chosen are within the vital range of zebrafish and correspond to temperatures that this species encounters in the natural environment. Previous results showed that the same treatment affects the brain proteome and the behaviour of adult zebrafish by producing alterations in the proteins involved in neurotransmitter release and synaptic function and impairing fish exploratory behaviour. In this study, we have investigated the performance of treated and control zebrafish during environmental exploration by using four behavioural tests (novel tank diving, light and dark preference, social preference and mirror biting) that are paradigms for assessing the state of anxiety, boldness, social preference and aggressive behaviour, respectively. The results showed that heat treatment reduces anxiety and increases the boldness of zebrafish, which spent more time in potentially dangerous areas of the tank such as the top and the uncovered bright area and at a distance from the social group, thus decreasing protection for the zebrafish. These data suggest that the increase in ambient temperature may compromise zebrafish survival rate in the natural environment.

show abstract

mentioning

confidence: 99%

Increase in environmental temperature affects exploratory behaviour, anxiety and social preference in Danio rerio

Angiulli¹,

Pagliara²,

Cioni³

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Modeling phenotypes associated with human neuropsychiatric disorders in animals is essential to gain a mechanistic understanding of the molecular and genetic players that influence the phenomenon and to devise intervention strategies (Lim and Mathuru, 2017). Zebrafish are used extensively in both cellular and molecular modeling of diseases (Bourque and Houvras, 2011; Santoriello and Zon, 2012; Ablain and Zon, 2013) as well as in pharmacological studies relying on behavioral assays (Cachat et al, 2010; Stewart et al, 2012, 2015; Norton, 2013; Kalueff, 2017; Bao et al, 2019; Fontana et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

An Automated Assay System to Study Novel Tank Induced Anxiety

Haghani

Karia

Cheng

et al. 2019

Front. Behav. Neurosci.

View full text Add to dashboard Cite

New environments are known to be anxiogenic initially for many animals including the zebrafish. In the zebrafish, a novel tank diving (NTD) assay for solitary fish has been used extensively to model anxiety and the effect of anxiolytics. However, studies can differ in the conditions used to perform this assay. Here, we report the development of an efficient, automated toolset and optimal conditions for effective use of this assay. Applying these tools, we found that two important variables in previous studies, the direction of illumination of the novel tank and the age of the subject fish, both influence endpoints commonly measured to assess anxiety. When tanks are illuminated from underneath, several parameters such as the time spent at the bottom of the tank, or the transitions to the top half of the tank become poor measures of acclimation to the novel environment. Older fish acclimate faster to the same settings. The size of the novel tank and the intensity of the illuminating light can also influence acclimation. Among the parameters measured, reduction in the frequency of erratic swimming (darting) is the most reliable indicator of anxiolysis. Open source pipeline for automated data acquisition and systematic analysis generated here and available to other researchers will improve accessibility and uniformity in measurements. They can also be directly applied to study other fish. As this assay is commonly used to model anxiety phenotype of neuropsychiatric ailments in zebrafish, we expect our tools will further aid comparative and meta-analyses.

show abstract

“…The zebrafish, as a model animal, has a nearly 70% similarities in genes between its genome and human genome [21], so exploring its brain-related dimorphism not only expands our understanding of the interaction between its reproductive processes and environmental stressors, but also has a positive effect on the analysis of human brain diseases [22].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling analysis of sex-based differentially expressed mRNAs and lncRNAs in the brains of mature zebrafish (Danio rerio)

Yuan

Jiang

Sun

et al. 2019

Preprint

View full text Add to dashboard Cite

KEYWORDSzebrafish, brain, the dimorphic patterns, mRNA and lncRNAs, high-throughput transcriptome sequencing 2 Abstract Background: Similar to humans, the zebrafish brain plays a central role in regulating sexual reproduction, maturation and sexual behavior. However, systematic studies of the dimorphic patterns of gene expression in the brain of male and female zebrafish are lacking. Results: In this study, the mRNA and lncRNAs expression profiles were obtained from the brain tissue samples of the three male and three female zebrafish by high-throughput transcriptome sequencing. We identified a total of 108 mRNAs and 50 lncRNAs with sex-based differential expression. We randomly selected four differentially expressed genes for RT-qPCR verification and the results certified that the expression pattern showed a similar trend between RNA-seq and RT-qPCR results. Protein-protein interaction network analysis, Gene Ontology (GO) analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed to obtain the biological significance of differentially expressed mRNA in the brain dimorphism of zebrafish. Finally, a Pearson correlation analysis was performed to construct the co-expression network of the mRNAs and lncRNAs. Conclusions: We found that 12 new lncRNAs not only have significant gender specificity in the brain of zebrafish, and this finding may provide a clue to further study of the functional difference between male and female zebrafish brain. Consent for publicationNot applicable. Publisher's NoteSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

show abstract

Opioid Neurobiology, Neurogenetics and Neuropharmacology in Zebrafish

Cited by 37 publications

References 185 publications

Increase in environmental temperature affects exploratory behaviour, anxiety and social preference in Danio rerio

Increase in environmental temperature affects exploratory behaviour, anxiety and social preference in Danio rerio

An Automated Assay System to Study Novel Tank Induced Anxiety

Transcriptome profiling analysis of sex-based differentially expressed mRNAs and lncRNAs in the brains of mature zebrafish (Danio rerio)

Contact Info

Product

Resources

About