Opioid control of the release of calcitonin gene-related peptide-like material from the rat spinal cord in vivo

Collin, E.; Frechilla, Diana; Pohl, Michel; Bourgoin, S.; Bars, D. Le; Hamon, Michel; François, Clément

doi:10.1016/0006-8993(93)90875-n

Cited by 60 publications

(26 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Immunoreactivity to DOR has been colocalized with CGRP immunoreactivity in dorsal root ganglion neurons and fibers (Dado et al 1993;Guo et al 1999). Moreover, KOR-and DOR-positive dorsal root ganglion neurons are sensitive to the VR1 agonist capsaicin (Zhang et al 1998) and activation of KOR decreases CGRP release into the intrathecal space (Collin et al 1993). VR1 and CGRP immunoreactivities are highly colocalized in trigeminal ganglia (Ichikawa and Sugimoto 2001), and the VR1 agonists capsaicin and anandamide evoke CGRP release from nerve terminals in the dorsal spinal cord (Tognetto et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Chemical coding of neurons expressing δ- and κ-opioid receptor and type I vanilloid receptor immunoreactivities in the porcine ileum

Poonyachoti

Kulkarni-Narla

Brown

2002

Cell and Tissue Research

View full text Add to dashboard Cite

Opioid drugs have profound antidiarrheal and constipating actions in the intestinal tract and are effective in mitigating abdominal pain. Mediators of intestinal inflammation and allergy produce increased mucosal secretion, altered bowel motility and pain due to their ability to evoke enteric secretomotor reflexes through primary afferent neurons. In this study, the distribution of delta- and kappa-opioid receptor (DOR and KOR, respectively) immunoreactivities in chemically identified neurons of the porcine ileum was compared with that of the capsaicin-sensitive type 1 vanilloid receptor (VR1). DOR and VR1 immunoreactivities were observed to be highly localized in choline acetyltransferase (ChAT)- and calcitonin gene-related peptide (CGRP)-positive neurons and nerve fibers of the submucosal and myenteric plexuses and both receptors exhibited frequent colocalization. In the inner submucosal plexus, they also were colocalized in substance P (SP)-positive neurons. Neurons in the outer submucosal plexus expressed DOR immunoreactivity alone or in combination with VR1. KOR-immunoreactive neurons were found only in the myenteric plexus; these cells coexpressed immunoreactivity to ChAT, CGRP, vasoactive intestinal peptide (VIP) or nitric oxide synthase (NOS). In addition, some KOR-positive neurons coexpressed immunoreactivities to DOR and VR1. Based on their neurochemical coding, opioid and vanilloid receptor-immunoreactive neurons in the submucosal and myenteric plexuses may include primary afferents and constitute novel therapeutic targets for the palliation of painful intestinal inflammatory, hypersensitivity and dysmotility states.

show abstract

Section: Discussionmentioning

confidence: 99%

Chemical coding of neurons expressing δ- and κ-opioid receptor and type I vanilloid receptor immunoreactivities in the porcine ileum

Poonyachoti

Kulkarni-Narla

Brown

2002

Cell and Tissue Research

View full text Add to dashboard Cite

show abstract

“…The cdcitonin gene related peptide (CGRP) is h s w n to zed and coreleased with SP from primary afferent fibers in the dorsal horn of the spinal cord (Gibson et d. 1981(Gibson et d. , 1984Woolf and Wiesenfeld-Wallin 11986;Collin et al 1993). Specific CGRP receptor binding sites are d s o h s w n to be concentrated in the dorsal horn of the spinal cord (Sexton et al 1986;Kruger et al 1988;Yashpd et d. 31992).…”

Section: Introductionmentioning

confidence: 99%

Alteration of calcitonin gene related peptide and its receptor binding sites during the development of tolerance to μ and δ opioids

Ménard

Rossum²,

Kar³

et al. 1995

Can. J. Physiol. Pharmacol.

View full text Add to dashboard Cite

Calcitonin gene related peptide (CGRP), one of the most abundant peptides in the spinal cord, is localized in primary afferents and released following nociceptive stimuli. Its colocalization and corelease with substance P, a well-known nociceptive neuropeptide, support the importance of CGRP in pain mechanisms. However, its distinctive function in that regard remains to be fully established. Recently, we reported that increases in CGRP-like immunostaining and decrements in specific 125I-labelled human CGRP alpha ([125I]hCGRP alpha) binding sites in the spinal cord were correlated with the development of tolerance to the spinal antinociceptive action of a mu opioid agonist, morphine. The goal of the present study was to investigate whether the development of tolerance to other classes of opioids, namely, delta and kappa agonists, can also alter CGRP-like immunostaining and receptors in the rat spinal cord. The antinociceptive effects of all opioids were monitored by the tail-immersion test. Tolerance to their antinociceptive properties was induced by the infusion for 7 days of mu (morphine sulfate, 7.5 micrograms/h), delta D([D-Pen2,D-Pen5]enkephalin (DPDPE), 2.0 micrograms/h), and kappa (U-50488H, 10.0 micrograms/h) related agonists at the spinal level (L4), using osmotic minipumps. We confirmed that rats chronically treated with morphine showed significant decreases in [125I]CGRP alpha binding in laminae I, II, and III of the L4 spinal cord, while CGRP-like immunostaining was increased in these same laminae. Similar effects were observed following a treatment with the delta agonist, DPDPE, while the kappa agonist, U-50488H, apparently only slightly decreased [125I]CGRP alpha] binding in lamina II. Binding in other laminae and CGRP-like immunostaining were not affected. These results suggest a specific interaction between spinal CGRP systems and the development of tolerance to the spinal antinociceptive effects of mu- and delta-related agonists.

show abstract

“…A study has shown that there was co-expression of muopioid receptor-like immunoreactivity with CGRP-or substance P-like immunoreactivity in rat trigeminal and dorsal root ganglion neurons; particularly, mu-opioid receptor-like immunoreactivity was found in axon terminals with CGRP-like immunoreactivity in laminae I and II of the medullary and the spinal dorsal horns (Li et al, 1998). It has also been reported that noxious stimulation resulted in the release of CGRP and substance P from primary afferent fibers in dorsal horn of the spinal cord (Duggan et al, 1988;Pohl et al, 1992) and that morphine and opioid peptides reduced the release of CGRP (Collin et al, 1993). Recently, we found that naloxone was able to attenuate the antinociception induced by intrathecal injection of CGRP8-37 in intact rats (Yu et al, 1995), in rats with mononeuropathy (Yu et al, 1996a), and in rats with inflammation (Yu et al, 1996b), indicating an involvement of opioid receptors in the CGRP8-37-induced antinociception in the spinal cord.…”

mentioning

confidence: 94%

Involvement of opioid receptors in the CGRP8‐37‐induced inhibition of the activity of wide‐dynamic‐range neurons in the spinal dorsal horn of rats

Yan

2004

J of Neuroscience Research

View full text Add to dashboard Cite

The present study was performed to explore the involvement of opioid receptors in the calcitonin gene-related peptide 8-37 (CGRP8-37, an antagonist of CGRP receptor)-induced inhibition of the activity of wide-dynamic-range (WDR) neurons in the spinal dorsal horn of rats. Extracellular recording was performed with a multibarrelled glass micropipette, and the chemicals were delivered by micro-iontophoresis. The discharge frequency of WDR neurons was evoked by subcutaneous electrical stimulation applied to the ipsilateral hindpaw. Iontophoretic application of CGRP8-37 by an ejection current of 160 nA induced significant inhibition of the discharge frequency of WDR neurons. The inhibitory effect of CGRP8-37 on the activity of WDR neurons was attenuated by later iontophoretic application of the opioid antagonist naloxone. Furthermore, the effect of CGRP8-37 was attenuated by either iontophoretic application of the kappa-receptor antagonist nor-binaltorphimine (nor-BNI) or the mu-receptor antagonist beta-funaltrexamine (beta-FNA) but not by the delta-receptor antagonist naltrindole. The results indicate that kappa- and mu-opioid receptors on the membrane of WDR neurons are involved in the modulation of CGRP8-37-induced antinociception in dorsal horn of the spinal cord in rats.

show abstract

Opioid control of the release of calcitonin gene-related peptide-like material from the rat spinal cord in vivo

Cited by 60 publications

References 52 publications

Chemical coding of neurons expressing δ- and κ-opioid receptor and type I vanilloid receptor immunoreactivities in the porcine ileum

Chemical coding of neurons expressing δ- and κ-opioid receptor and type I vanilloid receptor immunoreactivities in the porcine ileum

Alteration of calcitonin gene related peptide and its receptor binding sites during the development of tolerance to μ and δ opioids

Involvement of opioid receptors in the CGRP8‐37‐induced inhibition of the activity of wide‐dynamic‐range neurons in the spinal dorsal horn of rats

Contact Info

Product

Resources

About