Ophiostomatoid fungi can emit the bark beetle pheromone verbenone and other semiochemicals in media amended with various pine chemicals and beetle-released compounds

Cale, Jonathan A.; Ding, Rusong; Wang, Fuai; Rajabzadeh, Rahmatollah; Erbilgin, Nadir

doi:10.1016/j.funeco.2019.01.003

Cited by 32 publications

(45 citation statements)

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“…Furthermore, the similarities in FVOCs among different ophiostomatoid fungal species may also reflect a common ecological niche as fungi with a common ecological niche share similar FVOC profiles (Müller et al, 2013). While only detected from the headspace of G. clavigera, here, phenethyl acetate has been detected in FVOC profiles of some other ophiostomatoid fungi (e.g., L. longiclavatum, Endoconidiophora polonica) (Cale et al, 2016(Cale et al, , 2019Kandasamy et al, 2016). However, among ophiostomatoid fungi, cis-grandisol has only been reported from the FVOC profiles of G. clavigera (Cale et al, 2016(Cale et al, , 2019), suggesting species-specific production of FVOCs.…”

Section: Compoundsmentioning

confidence: 75%

“…The following experiments used the two isolates each for G. clavigera and O. ips that were reported in Cale et al (2019) and Wang et al (2020). For G. clavigera, both isolates (NOF2894 and NOF2896) were provided by the Northern Forestry Centre Culture Collection (Edmonton, Alberta) and were originally cultured from the phloem of MPB-infested lodgepole pine trees near Banff, Alberta (Rice et al, 2007).…”

Section: Fungal Growth and Volatile Collectionsmentioning

confidence: 99%

“…For G. clavigera, both isolates (NOF2894 and NOF2896) were provided by the Northern Forestry Centre Culture Collection (Edmonton, Alberta) and were originally cultured from the phloem of MPB-infested lodgepole pine trees near Banff, Alberta (Rice et al, 2007). The O. ips isolates (NOF1205 and NOF1284) were isolated from bark beetle galleries in lodgepole pine (Cale et al, 2019). Master cultures of the four isolates were grown on potato dextrose agar (PDA) at 22 • C in total darkness for 10 days.…”

Section: Fungal Growth and Volatile Collectionsmentioning

confidence: 99%

“…While six compounds were common between species, two compounds were detected only when G. clavigera grew alone. The six FVOCs (acetoin, ethyl acetate, isoamyl acetate, isobutanol, 2-methyl-1-butanol, and phenethyl alcohol) have also been detected in headspace of several ophiostomatoid fungal species, likely because they are byproducts of primary metabolism during vegetative growth (Kandasamy et al, 2016;Cale et al, 2019). Furthermore, the similarities in FVOCs among different ophiostomatoid fungal species may also reflect a common ecological niche as fungi with a common ecological niche share similar FVOC profiles (Müller et al, 2013).…”

Section: Compoundsmentioning

confidence: 99%

“…For instance, large variations in FVOCs can result from apparent interactions among abiotic factors such as temperature, humidity, and growth substrate (Polizzi et al, 2012). Likewise, VOCs originating from host plants and insects can influence emission of FVOCs (Cale et al, 2019). However, how ecological factors may affect the profiles of FVOC emitted is unknown.…”

Section: Introductionmentioning

confidence: 99%

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Exposure to Fungal Volatiles Can Influence Volatile Emissions From Other Ophiostomatoid Fungi

et al. 2020

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Fungal volatile organic compounds (FVOCs) can act as intra-and inter-kingdom communication signals that influence the growth and behaviors of organisms involved in antagonistic or mutualistic relationships with fungi. There is growing evidence suggesting that FVOCs can mediate interactions between organisms within and across different ecological niches. Bark beetles have established mutualistic relationships with ophiostomatoid fungi which can serve as a food source and condition host plant tissues for developing beetle larvae. While the profiles (both composition and concentrations) of volatile emission from ophiostomatoid fungi can be influenced by abiotic factors, whether emissions from a given fungal species can be influenced by those from another is still unknown. Here, we analyzed FVOCs emitted from the two ophiostomatoid fungi, Grosmannia clavigera and Ophiostoma ips, associated with mountain pine beetle and pine engraver beetle, respectively, when each fungus was growing alone or in a shared headspace. We used two isolates of each fungus species. Overall, we detected a total of eight volatiles in both G. clavigera alone or in combination with O. ips including acetoin, ethyl acetate, cis-grandisol, isoamyl alcohol, isobutanol, 2methyl-1-butanol, phenethyl acetate, and phenethyl alcohol. The profiles of volatiles emitted differed between the two fungal species but not between the two isolates of the same fungus. Six compounds were common between the species, whereas two compounds were detected only when G. clavigera was present. Moreover, the majority of volatiles were detected less frequently and at lower concentrations when the two fungi were grown together in a shared headspace. These results are likely due to reduced volatile emissions from O. ips in the presence of G. clavigera. However, changes in the profiles of fungal volatiles did not correspond with the observed changes in the growth of either species. Overall, these results suggest that the similarities in fungal volatiles among different species of fungi may reflect a common ecological niche and that the differences may correspond to species-specific adaptation to their respective host beetles or genetic factors.

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Section: Compoundsmentioning

confidence: 75%

Section: Fungal Growth and Volatile Collectionsmentioning

confidence: 99%

Section: Fungal Growth and Volatile Collectionsmentioning

confidence: 99%

Section: Compoundsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Exposure to Fungal Volatiles Can Influence Volatile Emissions From Other Ophiostomatoid Fungi

et al. 2020

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Screening of volatile organic compounds (VOCs) from liquid fungal cultures using ambient mass spectrometry

et al. 2023

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The potential of fungi for use as biotechnological factories in the production of a range of valuable metabolites, such as enzymes, terpenes, and volatile aroma compounds, is high. Unlike other microorganisms, fungi mostly secrete secondary metabolites into the culture medium, allowing for easy extraction and analysis. To date, the most commonly used technique in the analysis of volatile organic compounds (VOCs) is gas chromatography, which is time and labour consuming. We propose an alternative ambient screening method that provides rapid chemical information for characterising the VOCs of filamentous fungi in liquid culture using a commercially available ambient dielectric barrier discharge ionisation (DBDI) source connected to a quadrupole-Orbitrap mass spectrometer. The effects of method parameters on measured peak intensities of a series of 8 selected aroma standards were optimised with the best conditions being selected for sample analysis. The developed method was then deployed to the screening of VOCs from samples of 13 fungal strains in three different types of complex growth media showing clear differences in VOC profiles across the different media, enabling determination of best culturing conditions for each compound-strain combination. Our findings underline the applicability of ambient DBDI for the direct detection and comparison of aroma compounds produced by filamentous fungi in liquid culture. Graphical abstract

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Mutualistic Ophiostomatoid Fungi Equally Benefit from Both a Bark Beetle Pheromone and Host Tree Volatiles as Nutrient Sources

et al. 2021

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Ophiostomatoid fungi can emit the bark beetle pheromone verbenone and other semiochemicals in media amended with various pine chemicals and beetle-released compounds

Cited by 32 publications

References 50 publications

Exposure to Fungal Volatiles Can Influence Volatile Emissions From Other Ophiostomatoid Fungi

Exposure to Fungal Volatiles Can Influence Volatile Emissions From Other Ophiostomatoid Fungi

Screening of volatile organic compounds (VOCs) from liquid fungal cultures using ambient mass spectrometry

Mutualistic Ophiostomatoid Fungi Equally Benefit from Both a Bark Beetle Pheromone and Host Tree Volatiles as Nutrient Sources

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