Ophiostomatoid fungi associated with the northern spruce engraver, Ips perturbatus, in western Canada

Abstract

A number of ophiostomatoid fungi were isolated from the spruce-infesting bark beetle, Ips perturbatus and its galleries collected from felled spruce trees and logs in northern BC and the Yukon Territory. Isolates were identified to species using morphological characteristics, nuclear ribosomal DNA and partial beta-tubulin gene sequences. Thirteen morphological and phylogenetic species were identified among the isolates. Leptographium fruticetum, Leptographium abietinum, Ophiostoma bicolor, Ophiostoma manitobense, O. piceaperdum, and eight undescribed species of the genus Ophiostoma and the anamorph genera Leptographium, Hyalorhinocladiella, Ambrosiella and Graphium. A number of these species, i.e. L. fruticetum, Hyalorhinocladiella sp. 2, O. bicolor and O. manitobense, were isolated repeatedly from I. perturbatus, while others, i.e. Graphium sp. 1 and O. piceaperdum, seemed to be sporadic associates. Among all the isolates, L. fruticetum had the highest relative dominance in this survey. A high frequency of occurrence of this species with the beetle may indicate a specific relationship between the two partners.

Ophiostomatoid fungi (Ascomycota) associated with Pinus tabuliformis infested by Dendroctonus valens (Coleoptera) in northern China and an assessment of their pathogenicity on mature trees

“…The low recovery might also be related to the isolation methods (Kirisits 2004). Using fallen hosttraps and logs to collect the samples of either gallery system or the beetle itself could compensate for such deficiencies in order to investigate fully the ophiostomatoid fungi flora associated with beetle (e.g., Massoumi Alamouti et al 2007). …”

Target-specific PCR primers can detect and differentiate ophiostomatoid fungi from microbial communities associated with the mountain pine beetle Dendroctonus ponderosae

“…manitobensis and Cop. manitobensis-like from Ips perturbatus, which were isolated in Canada (Massoumi Alamouti et al 2007). All of the species found in Canada (MPB associates, Cop.…”

Three new <I>Graphium</I> species from baobab trees in South Africa and Madagascar

“…Isolates of all these species were collected from dead or wounded baobab trees and recognised based on DNA sequence and morphological comparisons. In addition to the SSU and ITS sequences used to delineate species in this group in the past (Okada et al 2000, Jacobs et al 2003, Geldenhuis et al 2004, Massoumi Alamouti et al 2007, Hulcr et al 2007), the TEF-1α gene sequence data for species in the G. penicillioides complex, including the three new species, are presented for the first time in this study.…”

“…This group includes the type species for the genus, G. penicillioides , and thus represents Graphium s.str. In addition to G. penicillioides , six known and seven undescribed species were recognised in the complex based on ITS sequences (Okada et al 2000, Geldenhuis et al 2004, Hulcr et al 2007, Massoumi Alamouti et al 2007, unpublished data in GenBank (GQ266157, GQ266158)). Most of these species have been isolated from insect galleries or exposed sapwood of trees (Jacobs et al 2003, Geldenhuis et al 2004, Hulcr et al 2007, Paciura et al 2010).…”

“…Graphium species are insect vectored (Jacobs et al 2003, Geldenhuis et al 2004, Massoumi Alamouti et al 2007, Hulcr et al 2007) and it is probable that there would be some relatedness among the insects that vector these fungi in Africa and in Madagascar. A study of these interactions would most likely lead to interesting observations about the biogeography and ecology of these microascalean fungi.…”