Ophiopogonin‑B targets PTP1B to inhibit the malignant progression of hepatocellular carcinoma by regulating the PI3K/AKT and AMPK signaling pathways

Abstract

ophiopogonin-B (oP-B) is a bioactive component from the root of Ophiopogon japonicus, which can exert anticancer effects on multiple malignant tumors. The present study aimed to uncover the effects of oP-B on hepatocellular carcinoma (Hcc) and the underlying mechanisms. an Hcc-xenografted mouse model was established and subsequently treated with oP-B (15 and 75 mg/kg) to observe the effects of oP-B on Hcc progression and protein tyrosine phosphatase 1B (PTP1B) expression in vivo. The Hcc cell line MHcc97-H was transfected with either PTP1B overexpression (ov)-PTP1B or empty vector control, and then exposed to different concentrations of oP-B. Subsequently, PTP1B expression, cell viability, proliferation, apoptosis, migration, invasion and angiogenesis were evaluated by western blotting, reverse transcription-quantitative Pcr, cell counting Kit-8, colony formation, Tunel staining, wound healing, Transwell and tube formation assays. The expression of phosphatidylinositol 3 kinase (Pi3K)/aKT and adenosine 5'-monophosphate-activated protein kinase (aMPK) was also assessed by western blot assay. The results showed that oP-B inhibited tumor growth and the expression of Ki67, cd31, VeGFa and PTP1B in Hcc xenograft model. The expression of PTP1B in Hcc cells was also inhibited by oP-B in a concentration-dependent manner. results from the in vitro studies revealed that oP-B suppressed cell proliferation, migration, invasion and angiogenesis, and promoted apoptosis of Hcc cells. However, PTP1B overexpression reversed the effect of oP-B on Hcc cells. Pi3K/aKT was inactivated and aMPK was activated by oP-B exposure in Hcc cells, and PTP1B overexpression blocked these effects. in conclusion, oP-B effectively inhibited the progression of Hcc both in vivo and in vitro. These effects may depend on downregulating PTP1B expression, thereby inactivating the Pi3K/aKT pathway and activating the aMPK pathway.

TCM targets ferroptosis: potential treatments for cancer

“…Ophiopogon in B (OP-B) is extracted from Radix Ophiopogon japonicus and has been reported to exert anticancer effects on different types of cancer, such as nasopharyngeal carcinoma (Dong et al, 2021), hepatocellular carcinoma (Yuan et al, 2022), lung cancer (Cheng et al, 2022). Previous findings have shown that OP-B has anticancer effects through the noniron ferroptosis pathway (Chen et al, 2016;Zhang et al, 2022).…”

Critical roles of PTPN family members regulated by non-coding RNAs in tumorigenesis and immunotherapy

“…Specifically, Pituitary homeobox 1 (PITX1) exerts a tumor suppressive effect in hepatocarcinogenesis through regulation of Ras guanosine triphosphatase-activating protein (p120RasGAP) expression levels, but PTPN1 attenuates the protein stability of PITX by directly dephosphorylating PITX1 at residues Y160, Y175 and Y179 ( 144 ). Furthermore, down-regulation of PTPN1 expression inhibits HCC progression possibly by inactivating the PI3K/AKT pathway and activating the AMPK pathway ( 145 ).…”