Operational characteristics of an antibody-immobilized QCM system detecting Salmonella spp.

Park, In-Seon; Kim, Woo-Yeon; Kim, Namsoo

doi:10.1016/s0956-5663(00)00053-1

Cited by 99 publications

(27 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This label-free biosensor achieved a response range to E. coli concentrations from 10 5 to 10 8 cfu/ml, with a detection limit of 10 6 cfu/ml. Although the detection of this label-free biosensor was not as low as that of the impedance biosensor with enzyme amplification (Ruan et al, 2002), its detection limit was comparable with other label-free immunosensors for detection of pathogenic bacteria using different transducer techniques, including QCM (quartz crystal microbalance) immunosensors for detection of Salmonella with detection limits of 3.2 × 10 6 cfu/ml and 9.9 × 10 5 cfu/ml (Park et al, 2000;Park and Kim, 1998), SPR (surface plasmon resonance) immonusensors for detection of Salmonella enteritidis and L. monocytogens with detection limits of 10 6 cfu/ml (Koubova et al, 2001), and a SPR sensor for detection of E. coli O157:H7 with a detection limit of 10 7 cfu/ml (Fratamico et al, 1998). Apparently, the response range and the detection limit of the biosensor could be optimized by the size, dimension, and design of the IDA.…”

Section: Electrochemical Impedance Biosensors Using Redox Probesmentioning

confidence: 81%

Food‐borne Pathogenic Bacteria

Shi

Xie

Zhou

2017

Food Safety in China

View full text Add to dashboard Cite

The realization of rapid, sensitive, and specific methods to detect foodborne pathogenic bacteria is central to implementing effective practice to ensure food safety and security. As a principle of transduction, the impedance technique has been applied in the field of microbiology as a means to detect and/or quantify foodborne pathogenic bacteria. The integration of impedance with biological recognition technology for detection of bacteria has led to the development of impedance biosensors that are finding wide-spread use in the recent years. This paper reviews the progress and applications of impedance microbiology for foodborne pathogenic bacteria detection, particularly the new aspects that have been added to this subject in the past few years, including the use of interdigitated microelectrodes, the development of chip-based impedance microbiology, and the use of equivalent circuits for analysis of the impedance systems. This paper also reviews the significant developments of impedance biosensors for bacteria detection in the past 5 years, focusing on microfabricated microelectrodes-based and microfluidic-based Faradaic electrochemical impedance biosensors, non-Faradaic impedance biosensors, and the integration of impedance biosensors with other techniques such as dielectrophoresis and electropermeabilization. Published by Elsevier Inc.

show abstract

Section: Electrochemical Impedance Biosensors Using Redox Probesmentioning

confidence: 81%

Food‐borne Pathogenic Bacteria

Shi

Xie

Zhou

2017

Food Safety in China

View full text Add to dashboard Cite

show abstract

“…This device can be utilized to quantify cells in suspensions other than impedance microbiology and impedance biosensors for bacteria detection, since the detection limit of this method is comparable with other sensors. The reported sensor for detection of pathogenic bacteria are quartz crystal microbalance (QCM) immunosensors for detection of Salmonella with detection limits of 9.9 × 10 5 cfu/mL [28], surface plasmon resonance (SPR) sensor for the detection of E. coli O157:H7 with a detection limit of 10 7 cfu/mL [29] and SPR immunosensors for the detection of Salmonella enteritidis and Listeria monocytogenes with detection limits of 10 6 cfu/mL [30].…”

Section: Resultsmentioning

confidence: 99%

Electrical Impedance Spectroscopy for Detection of Cells in Suspensions Using Microfluidic Device with Integrated Microneedles

et al. 2017

View full text Add to dashboard Cite

Abstract:In this study, we introduce novel method of flow cytometry for cell detection based on impedance measurements. The state of the art method for impedance flow cytometry detection utilizes an embedded electrode in the microfluidic to perform measurement of electrical impedance of the presence of cells at the sensing area. Nonetheless, this method requires an expensive and complicated electrode fabrication process. Furthermore, reuse of the fabricated electrode also requires an intensive and tedious cleaning process. Due to that, we present a microfluidic device with integrated microneedles. The two microneedles are placed at the half height of the microchannel for cell detection and electrical measurement. A commercially-available Tungsten needle was utilized for the microneedles. The microneedles are easily removed from the disposable PDMS (Polydimethylsiloxane) microchannel and can be reused with a simple cleaning process, such as washing by ultrasonic cleaning. Although this device was low cost, it preserves the core functionality of the sensor, which is capable of detecting passing cells at the sensing area. Therefore, this device is suitable for low-cost medical and food safety screening and testing process in developing countries.

show abstract

“…They are simple to use and economical methods. They measure changes due to mass deposits which are produced by antigenantibody interaction and are not susceptible to interferences caused by various coloring materials in the samples [15,28,29].…”

Section: Resultsmentioning

confidence: 99%