2001
DOI: 10.1006/cryo.2001.2343
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Operating Conditions That Affect the Resistance of Lactic Acid Bacteria to Freezing and Frozen Storage

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Cited by 96 publications
(52 citation statements)
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“…Although freeze drying is the conventional drying technique used commercially by starter culture manufactures, it is lengthy and more expensive than other drying processes (Fonseca et al 2001, Ampatzoglou et al 2010, Morgan et al 2006). Many attempts have been made to develop alternative drying processes at lower cost and some authors have reported reasonable cell viability after drying (Tymczyszyn et al 2008).…”
Section: Introductionmentioning
confidence: 99%
“…Although freeze drying is the conventional drying technique used commercially by starter culture manufactures, it is lengthy and more expensive than other drying processes (Fonseca et al 2001, Ampatzoglou et al 2010, Morgan et al 2006). Many attempts have been made to develop alternative drying processes at lower cost and some authors have reported reasonable cell viability after drying (Tymczyszyn et al 2008).…”
Section: Introductionmentioning
confidence: 99%
“…Freezing and storage at low temperatures (ϽϪ40°C) are commonly applied to preserve the viability of concentrates while maintaining their technological properties upon thawing (acidification activity, production of aroma compounds, and contribution to product texture). However, bacterial resistance to freezing and to frozen storage depends on the strain, culture conditions before freezing, harvesting, formulation (type and concentration of cryoprotectant), freezing conditions, and final storage temperature (9).…”
mentioning
confidence: 99%
“…Glycerol was added to the supernatant obtained after centrifugation of the fermented medium, since it has been reported to be a good cryoprotectant of LAB (9,17,33). The final concentration of glycerol in the supernatant was 10% (wt/wt).…”
mentioning
confidence: 99%
“…The process of freezing appeared to have different effects on different LAB as well as different effects on strains within the same genus. Moreover, the freezing response of the strains depends on the time of the cold shock process and the induction of cryotolerance appears to be dependent on the growth phase in which the cold shock took place [43][44][45][46][47].…”
Section: Principal Responses To the Most Common Stressesmentioning
confidence: 99%