OpenSpinMicroscopy: an open-source integrated microscopy platform

Gualda, Emilio J.; Vale, Tiago Gurgel do; Almada, Pedro; Feijó, José A.; Martins, Gabriel G.; Moreno, Nuno

doi:10.1038/nmeth.2508

Cited by 112 publications

(83 citation statements)

References 6 publications

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“…Microscopy modalities based on this hardware arrangement are either commercially available (Lightsheet Z.1 from Carl Zeiss) or can be constructed in place directly according to the OpenSPIM concept (http:// openspim.org). The OpenSPIM concept was developed to adapt modular SPIM systems to the particular requirements of end users (Gualda et al, 2013;Pitrone et al, 2013). The concept is well supported by the community and provides a broad range of biological applications (Girstmair et al, 2016).…”

Section: Lsfmmentioning

confidence: 99%

Advances in Imaging Plant Cell Dynamics

et al. 2017

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Section: Lsfmmentioning

confidence: 99%

Advances in Imaging Plant Cell Dynamics

et al. 2017

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“…In addition, LSFM has been combined with SR techniques such as STORM [48,[80][81][82], STED [92], and structured illumination [69,75,93]. The versatility of LSFM also allows combining it with more established techniques, such as FLIM [94][95][96], FRET [96], hyperspectral fluorescence [97], Raman [98][99][100][101], and OPT [102,103]. The combined advantages of LSFM are thus shared with multimodal approaches and allow extracting structural, functional, chemical, and molecular information from the sample, enabling a comprehensive interpretation of the biological process of study.…”

Section: 1g Lsfm Allows Multimodal Imaging Of Biological Samplesmentioning

confidence: 99%

Light-sheet microscopy: a tutorial

et al. 2018

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This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from an optics perspective. As such, emphasis is placed on the advantages that LS microscope configurations present, given the degree of freedom gained by uncoupling the excitation and detection arms. The new imaging properties are first highlighted in terms of optical parameters and how these have enabled several biomedical applications. Then, the basics are presented for understanding how a LS microscope works. This is followed by a presentation of a tutorial for LS microscope designs, each working at different resolutions and for different applications. Then, based on a numerical Fourier analysis and given the multiple possibilities for generating the LS in the microscope (using Gaussian, Bessel, and Airy beams in the linear and nonlinear regimes), a systematic comparison of their optical performance is presented. Finally, based on advances in optics and photonics, the novel optical implementations possible in a LS microscope are highlighted.

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“…Another DIY open platform light sheet microscopy system called the "OpenSpinMicroscopy project" has also been launched [24]. The OpenSpinMicroscopy project also makes freely available blueprints on how to build the microscope together with operating instructions and data handling/processing software, through its website (https://sites.google.com/site/openspinmicroscopy/).…”

Section: Fig3 Basic Layout Of a Light Sheet Microscopementioning

confidence: 99%

Optical Microscopy Has Evolved To Overcome Limitations in Resolution and Image Clarity, (An Overview of Some Available Options)

2016

4th International Conference on Advances in Agricultural, Biological &Amp; Ecological Sciences (AABES-16) Dec. 1-2, 2016 London

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Abstract-Optical microscopy has served the BiologicalSciences as an invaluable tool for visualization of very small structures. Researchers in the biological sciences can harness recent improvements in optical microscopy to provide new insights in their own fields. The limitation that light diffraction places on optical resolution has been met by exploiting Extreme Ultra Violet [EUV] Radiation but this has also introduced new challenges. The problem posed by out of focus light from points outside the focal plane has been met by the development of Light Sheet Fluorescent Microscopy which also helps minimize photo-toxicity in living cells. Some of the options available to researchers to exploit these advances are discussed.

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OpenSpinMicroscopy: an open-source integrated microscopy platform

Cited by 112 publications

References 6 publications

Advances in Imaging Plant Cell Dynamics

Advances in Imaging Plant Cell Dynamics

Light-sheet microscopy: a tutorial

Optical Microscopy Has Evolved To Overcome Limitations in Resolution and Image Clarity, (An Overview of Some Available Options)

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