Open tubular capillary electrokinetic chromatography in etched fused-silica tubes

Pesek, Joseph J.; Matyska, Maria T.

doi:10.1016/s0021-9673(00)00180-1

Cited by 72 publications

(49 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These separations were performed using an octadecylsilane capillary column. Since then, several preparation methods of open-tubular columns have gone through extensive research [19,32,77,[98][99][100].…”

Section: Preparation Of Open-tubular Columns and Applications To Analmentioning

confidence: 99%

“…This creates radial extensions from the wall that decrease the distance an analyte has to travel in order to interact with the stationary phase [96]. The etching process was first introduced by Onuska et al [153] for gas chromatography (GC), and it was then modified for OT-CEC by Pesek et al [3,28,30,[77][78][79][80][154][155][156][157][158][159][160][161][162].…”

Section: Chemical Bonding After Etchingmentioning

confidence: 99%

“…In recent years, a large number of reviews have been provided on the general developments and applications of CEC [4,17,19,32,33,76,77,[84][85][86][87][88][89][90][91][92][93][94][95][96][97][98][99][100][101]. In contrast, the review provided here focuses primarily on major developments in the area of OT-CEC.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Analytical separations in open‐tubular capillary electrochromatography

Kapnissi‐Christodoulou

Zhu

Warner

2003

Electrophoresis

View full text Add to dashboard Cite

This review represents a summary of recent progress in open-tubular capillary electrochromatography (OT-CEC) for chiral and achiral separations. The OT-CEC approach is an alternative to packed-CEC that could eliminate the problems associated with retaining frits and silica particles. In OT-CEC, the stationary phase is immobilized on the inner walls of the capillary. Preparation of the stationary phase is critical for OT-CEC. The preparation methods for capillary columns include (i) adsorption, (ii) covalent bonding and/or cross-linking, (iii) porous layers, (iv) chemical bonding after etching, (v) sol-gel, and (vi) molecular imprinting. Major developments, potential applications, technical difficulties and advantages associated with these wall coatings in OT-CEC are presented. In addition, the coupling of OT-CEC with mass spectrometry (MS) is briefly reviewed. Several applications of this hyphenated technique for analytical separations are also summarized.

show abstract

Section: Preparation Of Open-tubular Columns and Applications To Analmentioning

confidence: 99%

Section: Chemical Bonding After Etchingmentioning

confidence: 99%

See 1 more Smart Citation

Analytical separations in open‐tubular capillary electrochromatography

Kapnissi‐Christodoulou

Zhu

Warner

2003

Electrophoresis

View full text Add to dashboard Cite

show abstract

“…The etched capillaries showed better resolution and longer retention than treated fused silica. Four proteins were separated, using a capillary coated with 1% Polybrene [158]. Etched fused silica with 20, 50, and 75 mm ID where further chemically modified by silization/hydrosilation to attach a C 18 , diol and cholesterol [159].…”

Section: Enantioseparationmentioning

confidence: 99%

Recent progress in high‐performance capillary bioseparations

et al. 2003

View full text Add to dashboard Cite

Bioanalysis is a fast growing domain with new technological developments in the field of mass spectrometry, separation science, and bioinformatics. The central role of separation prior to detection and evaluation of analytes is indisputable. In fact, since the decipherment of the human genome via multichannel capillary electrophoresis in combination with laser-induced fluorescence [1], the analysis of the proteome, i.e., the type and amount of all proteins expressed at a certain time by the genome in a cell or tissue [2] became of central interest. Therefore, new fast and sensitive capillary liquid chromatographic, electrophoretic, and electrochromatographic separation techniques, suitable for high-throughput analysis of proteins, peptides and nucleic acids, are required.

show abstract

“…In open tubular CEC format, the stationary phase is fixed to the inner wall of a capillary column. Although smaller and smaller internal diameter capillaries [9], various etching processes [10], and improved coating technologies [11] have been employed, the surface-tovolume ratio of these columns is small leading to low ana-lyte retention and reduced sample capacities. As a result, its application in areas that require intensive sample interaction with the stationary phase has been limited.…”

Section: Introductionmentioning

confidence: 99%

Capillary-based solid-phase extraction with columns prepared using different bead trapping methods

Xie

Oleschuk

2005

Electrophoresis

View full text Add to dashboard Cite

Three approaches of bead immobilization for CEC column preparation in a capillary were examined for SPE. The three approaches included a packed column with a single frit, a packed column with an inlet and outlet frit, and an entrapped column where beads were immobilized within an organic polymer. A direct comparison of SPE/preconcentration of 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene and 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionic acid with a 2 cm long bed showed that the entrapped column yielded the best performance in terms of reproducibility and robustness. The room temperature chemistry utilized to form the entrapped column enables the column to be photopatterned anywhere within the capillary without loss in bead functionality, and effectively links individual beads to one another at specific bead-bead and bead-capillary contact points. A 0.5 cm long entrapped bed exhibits high mechanical strength and is able to withstand >4400 psi. The entrapped bed was used to preconcentrate progesterone and beta-estradiol providing signal enhancements of >600. Following preconcentration, the hormones could be separated using CEC. With the current availability of numerous well-characterized chromatographic packing materials and the relative simplicity of the fabrication method, this methodology can be readily adapted to HPLC, CEC, and micro total analysis system.

show abstract

Open tubular capillary electrokinetic chromatography in etched fused-silica tubes

Cited by 72 publications

References 31 publications

Analytical separations in open‐tubular capillary electrochromatography

Analytical separations in open‐tubular capillary electrochromatography

Recent progress in high‐performance capillary bioseparations

Capillary-based solid-phase extraction with columns prepared using different bead trapping methods

Contact Info

Product

Resources

About