Open-Source Pressure Controller Based on Compact Electro-Pneumatic Regulators for Droplet Microfluidics Applications

Filatov, N A; Denisov, Ivan A.; Evstrapov, A. A.; Bukatin, Anton

doi:10.1109/tim.2022.3158383

Cited by 5 publications

(1 citation statement)

References 58 publications

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“…All reagents were sampled into the microfluidic chip at constant pressures in the range of 0–50 kPa using four ITV0010 electro-pneumatic regulators (SMC, Tokyo, Japan) assembled into the microfluidic pressure controller [ 55 ]. These regulators were connected to the chip via 15 mL tubes (for basic and starting solutions) and a 50 mL tube (for oil-based medium).…”

Section: Methodsmentioning

confidence: 99%

Droplet Microfluidic Device for Chemoenzymatic Sensing

et al. 2022

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The rapid detection of pollutants in water can be performed with enzymatic probes, the catalytic light-emitting activity of which decreases in the presence of many types of pollutants. Herein, we present a microfluidic system for continuous chemoenzymatic biosensing that generates emulsion droplets containing two enzymes of the bacterial bioluminescent system (luciferase and NAD(P)H:FMN–oxidoreductase) with substrates required for the reaction. The developed chip generates “water-in-oil” emulsion droplets with a volume of 0.1 μL and a frequency of up to 12 drops per minute as well as provides the efficient mixing of reagents in droplets and their distancing. The bioluminescent signal from each individual droplet was measured by a photomultiplier tube with a signal-to-noise ratio of up to 3000/1. The intensity of the luminescence depended on the concentration of the copper sulfate with the limit of its detection of 5 μM. It was shown that bioluminescent enzymatic reactions could be carried out in droplet reactors in dispersed streams. The parameters and limitations required for the bioluminescent reaction to proceed were also studied. Hereby, chemoenzymatic sensing capabilities powered by a droplet microfluidics manipulation technique may serve as the basis for early-warning online water pollution systems.

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Section: Methodsmentioning

confidence: 99%

Droplet Microfluidic Device for Chemoenzymatic Sensing

et al. 2022

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Surface behaviors of droplet manipulation in microfluidics devices

Guo²,

Liu³

2022

Advances in Colloid and Interface Science

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Microfluidics-Driven Dripping Technique for Fabricating Polymer Microspheres Doped with AgInS₂/ZnS Quantum Dots

Kurassova,

Filatov,

Karamysheva

et al. 2024

ACS Omega

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Fluorescent microspheres are at the forefront of biosensing technologies. They can be used for a wide range of biomedical applications. They consist of organic dyes and polymers, which are relatively immune to photobleaching and other environmental factors. However, recently developed AgInS 2 /ZnS quantum dots are a water-soluble, low-toxicity class of semiconductor nanocrystals with enhanced stability as fluorescent materials. Here, we propose a simple way for making microspheres: a microfluidic dripping technique for acrylamide polymer spheres doped with quantum dots. Analyses of their spectra show that the emission of quantum dots, dispersed in water, is saturated with an increasing pump intensity, while quantum dots embedded into polymer microspheres exhibit a more sustained emission. Moreover, our study unveils a remarkable reduction in the luminescence lifetime of quantum dots embedded in microspheres: the mean value of the decay time for quantum dots in solutions was 91 and 3.5 ns for similar quantum dots incorporated into polymer microspheres.

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Open-Source Pressure Controller Based on Compact Electro-Pneumatic Regulators for Droplet Microfluidics Applications

Cited by 5 publications

References 58 publications

Droplet Microfluidic Device for Chemoenzymatic Sensing

Droplet Microfluidic Device for Chemoenzymatic Sensing

Surface behaviors of droplet manipulation in microfluidics devices

Microfluidics-Driven Dripping Technique for Fabricating Polymer Microspheres Doped with AgInS₂/ZnS Quantum Dots

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Open-Source Pressure Controller Based on Compact Electro-Pneumatic Regulators for Droplet Microfluidics Applications

Cited by 5 publications

References 58 publications

Droplet Microfluidic Device for Chemoenzymatic Sensing

Droplet Microfluidic Device for Chemoenzymatic Sensing

Surface behaviors of droplet manipulation in microfluidics devices

Microfluidics-Driven Dripping Technique for Fabricating Polymer Microspheres Doped with AgInS2/ZnS Quantum Dots

Contact Info

Product

Resources

About

Microfluidics-Driven Dripping Technique for Fabricating Polymer Microspheres Doped with AgInS₂/ZnS Quantum Dots