Open-pulled straw vitrification differentiates cryotolerance of in vitro cultured rabbit embryos at the eight-cell stage

Lin, Tsung-Yi; Chen, C.H.; Sung, Li Ying; Carter, M.G.; Chen, Y.E.; Du, Fuliang; Ju, Jyh-Cherng; Xu, Jie

doi:10.1016/j.theriogenology.2010.10.018

Cited by 23 publications

(32 citation statements)

References 57 publications

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“…As referred, the initial stages of development are those with higher cryosensitivity, from oocytes to late embryo, with the acquisition of cryotolerance in an intermediate stage of development. Accordingly, Garcia-Garcia et al [167] and Lin et al [198] found differences in the cryotolerance of IVP embryos vitrified by OPS, depending on its developmental stage, with better results in morulae/ blastocyst, defining the eight-cell stage as the critical point for acquiring cryotolerance. Therefore, it is not surprising that, in the last years of research, cryopreservation techniques have focused mainly in embryos at morulae and blastocyst stages and less in oocytes or zygotes.…”

Section: Methods Of Improving the Cryopreservation Of Sheep Embryosmentioning

confidence: 99%

Cryopreservation of Sheep Produced Embryos – Current and Future Perspectives

Romão¹,

Marques²,

Bettencourt³

et al. 2016

Insights From Animal Reproduction

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Due to economical and scientific limitations, sheep embryo reproductive technologies are less commercially applied than in other animal species. However, it is very clear that, in the near future, those techniques are expected to have a central role in animal production as a consequence of genetic and reproductive demands. One drawback is that results obtained after sheep embryo cryopreservation are unattractive for commercial purposes. It is expected that a successful cryopreservation of sheep embryos can push forward all other reproductive biotechnologies in this species, such as multiple ovulation and embryo transfer (MOET), artificial insemination, or in vitro production of embryos. This paper tries to discuss the current and future perspectives of cryopreservation of in vivo-and in vitro-produced sheep embryos concerning advantages and limitations for its practical use and possible solutions for improving methods to allow a higher survival rate of cryopreserved embryos.

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Section: Methods Of Improving the Cryopreservation Of Sheep Embryosmentioning

confidence: 99%

Cryopreservation of Sheep Produced Embryos – Current and Future Perspectives

Romão¹,

Marques²,

Bettencourt³

et al. 2016

Insights From Animal Reproduction

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show abstract

“…Previously, we reported cryopreservation of different stages of rabbit embryos with satisfactory in vitro and in vivo results (Lin et al, 2011). We also established pluripotent rabbit ESCs using fresh embryos (Xue et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…T he rabbit is a classical model animal species (Fan and Watanabe, 2003;Fan et al, 2015;Intawicha et al, 2009;Lin et al, 2011). It has a short gestation period (30-31 days) and large litter size (4-12/litter) and can be housed conveniently in an indoor facility.…”

Section: Introductionmentioning

confidence: 99%

Derivation of Rabbit Embryonic Stem Cells from Vitrified–Thawed Embryos

Chen

et al. 2015

Cellular Reprogramming

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The rabbit is a useful animal model for regenerative medicine. We previously developed pluripotent rabbit embryonic stem cell (rbESC) lines using fresh embryos. We also successfully cryopreserved rabbit embryos by vitrification. In the present work, we combined these two technologies to derive rbESCs using vitrified-thawed (V/T) embryos. We demonstrate that V/T blastocysts (BLs) can be used to derive pluripotent rbESCs with efficiencies comparable to those using fresh BLs. These ESCs are undistinguishable from the ones derived from fresh embryos. We tested the developmental capacity of rbESCs derived from V/T embryos by BL injection experiments and produced chimeric kits. Our work adds cryopreservation to the toolbox of rabbit stem cell research and applications and will greatly expand the available research materials for regenerative medicine in a clinically relevant animal model.

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“…Furthermore, the pronuclear-stage embryos used for microinjection do not lend themselves well to cryopreservation, and a significant loss of viable embryos often results. 13 In an early study, Chang 14 successfully stored fertilized rabbit ova at a low temperature for several days. Later, Hughes and Anderson 15 established a simple method for storing rabbit embryos under conditions that could be easily maintained in an ordinary refrigerator set at 48C.…”

mentioning

confidence: 99%

Delaying embryo development by storing at 4°C for synchronization to recipients in microinjection technique in rabbits

et al. 2013

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Short-term storage of embryos at low temperature induces developmental arrest of the embryo and would appear to be a valuable aid in embryo-transfer techniques to avoid wasting embryos. Embryo storage at 48C was examined to allow synchronization with embryo-transfer recipients using the microinjection technique. Superovulation was induced in female Japanese White donor rabbits four days before mating with males. At the same time, control recipients were injected with human chorionic gonadotropin (hCG) to allow synchronization (R1); the hCG injections were delayed by 24 h in the experimental group (R2). DNA constructs for expressing human C-reactive protein or apolipoprotein AII were microinjected into the male pronuclei of the ova. The microinjected embryos were immediately transferred to recipients (R1) or stored at 48C in phosphate-buffered saline containing 10% fetal bovine serum. After 17-20 h, the stored embryos were incubated at 378C for one hour, and the morphologically normal embryos were transferred to recipients (R2). In the R1 rabbits, 855 embryos were transferred to 29 recipients, and 72.4% of the recipients became pregnant. Seven of the 84 offspring were transgenic. In the R2 rabbits, 478 embryos were transferred to 16 recipients, and 62.5% of the recipients became pregnant. Two of the 39 offspring were transgenic. There were no differences in pregnancy rate, litter size and transgenic integration rate between R1 and R2. These results suggest that the short-term 48C storage of microinjected embryos can be a valuable method for synchronization with recipients, and reducing wastage of embryos and the sacrifice of rabbits.

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Open-pulled straw vitrification differentiates cryotolerance of in vitro cultured rabbit embryos at the eight-cell stage

Cited by 23 publications

References 57 publications

Cryopreservation of Sheep Produced Embryos – Current and Future Perspectives

Cryopreservation of Sheep Produced Embryos – Current and Future Perspectives

Derivation of Rabbit Embryonic Stem Cells from Vitrified–Thawed Embryos

Delaying embryo development by storing at 4°C for synchronization to recipients in microinjection technique in rabbits

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