Open millifluidics based on powder-encased channels

Li, Xiaoguang; Pang, Xianglong; Jiang, Haohao; Duan, Mei; Liu, Heng; Yang, Z. Gary; Xi, Yuhang; Russell, Thomas P.

doi:10.1073/pnas.2302907120

Cited by 3 publications

(3 citation statements)

References 32 publications

(40 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because of interfacial jamming, an LP can be easily cut by a hydrophobic tool into pieces without leaking its inner liquid. [18,19,23,37,48] In the experiment here, we cut a group of identical rod-shaped LPs with each into pre-defined piece numbers, by running a plastic pipette across the LP body (Figure 5a). The surface NPs rearranged to cover the newly exposed surfaces, and the areal particle density decreased gradually with the number of segmentation times, relieving the surface jamming such that the daughter LPs from multiple segmentations tended to exhibit rounded profiles (Figure 5b).…”

Section: Exploration On the Pdt Efficacy Attenuation Using The Lp's C...mentioning

confidence: 99%

“…Recently, increasing attention has been paid to miniature liquid reactors played by particle-encased liquid constructs, including spherical "liquid marbles (LMs)" [15][16][17] and complexly shaped "liquid plasticines" (LPs), [18] as they are able to circumvent the limitations of solid reactors based on their peculiar properties of openness, cuttability, reconfigurability, among others. [19,20] Attractive achievements have been made using these liquid reactors in many areas including 3D cell culture, [21,22] protein separation and analysis, [23] surface enhanced Raman spectroscopy, [24,25] chemical synthesis, [26] CO 2 capture, [27] and sensors. [28,29] The PDT area has been also accessed in which a LM minireactor was demonstrated to surpass the commonly used petri dish in the resultant efficacy of PDT, owing to the greater specific surface area that led to the use of more light.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Rod‐Shaped Liquid Plasticine as Cuttable Minireactor for Photodynamic Therapy of Tumors

Liu,

Peng,

Guo

et al. 2024

Small

Self Cite

View full text Add to dashboard Cite

Photodynamic therapy (PDT) has emerged as a promising non‐invasive approach for cancer treatment. Enhancing its efficacy and understanding its absorption‐induced attenuation are significant while the solutions are limited, particularly for the latter. In this study, a rod‐shaped liquid plasticine (LP), comprised of a tumor cell solution encased by a nanoparticle monolayer, is used to serve as a powerful minireactor for addressing these issues. The channel structure, openness, and cuttability of the LP reactor are exploited for providing benefits to PDT. The resulting PDT efficacy is several times higher than those from droplet reactors with common spherical shapes. The attenuation law, which is fundamental in PDT yet poorly understood due to the lack of experimental approaches, is preliminarily uncovered here from the perspective of in vitro experiments by using the LP's cuttability, affording quantitative understanding on this difficult subject. These findings provide insights into the widely‐concerned topics in PDT, and highlight the great potential of an LP reactor in offering innovation power for the biochemical and biomedical arenas.

show abstract

Section: Exploration On the Pdt Efficacy Attenuation Using The Lp's C...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rod‐Shaped Liquid Plasticine as Cuttable Minireactor for Photodynamic Therapy of Tumors

Liu,

Peng,

Guo

et al. 2024

Small

Self Cite

View full text Add to dashboard Cite

show abstract

“…Advances in millifluidics [32,33] allow the automatization of standard cell culture procedures, including periodic sample collection under sterile conditions [34][35][36]. This approach can obtain enough samples from regular transwell barrier cultures to determine their time-resolved transport kinetics comparable to the data obtainable from microfluidic Franz chambers.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Analysis of a Pilot Transwell Barrier Model with Automated Sampling and Mathematical Modeling

Tárnoki-Zách,

Bősze,

Czirók

2023

Pharmaceutics

View full text Add to dashboard Cite

In the preclinical phase of drug development, it is necessary to determine how the active compound can pass through the biological barriers surrounding the target tissue. In vitro barrier models provide a reliable, low-cost, high-throughput solution for screening substances early in the drug candidate development process, thus reducing more complex and costly animal studies. In this pilot study, the transport properties of TB501, an antimycobacterial drug candidate, were characterized using an in vitro barrier model of VERO E6 kidney cells. The compound was delivered into the apical chamber of the transwell insert, and its concentration passing through the barrier layer was measured through the automated sampling of the basolateral compartment, where media were replaced every 30 min for 6 h, and the collected samples were stored for further spectroscopic analysis. The kinetics of TB501 concentration obtained from VERO E6 transwell cultures and transwell membranes saturated with serum proteins reveal the extent to which the cell layer functions as a diffusion barrier. The large number of samples collected allows us to fit a detailed mathematical model of the passive diffusive currents to the measured concentration profiles. This approach enables the determination of the diffusive permeability, the diffusivity of the compound in the cell layer, the affinity of the compound binding to the cell membrane as well as the rate by which the cells metabolize the compound. The proposed approach goes beyond the determination of the permeability coefficient and offers a more detailed pharmacokinetic characterization of the transwell barrier model. We expect the presented method to be fruitful in evaluating other compounds with different chemical features on simple in vitro barrier models. The proposed mathematical model can also be extended to include various forms of active transport.

show abstract

How Wide and High can Polyhedral Liquid Marbles be Fabricated?

Hayashi,

Manabe,

Hirai

et al. 2024

Adv Materials Inter

View full text Add to dashboard Cite

Liquid marbles (LMs) are liquid droplets in gaseous phase, which are generally millimeter size, coated by solid particles. In this study, how wide and high LMs can be fabricated is investigated. Polyhedral LMs are fabricated using polymer plates with millimeter to meter sizes as a stabilizer and water as an inner liquid. Rectangular LMs with widths exceeding 1 m, the largest width ever reported, can be successfully fabricated. The height of the LMs is found to be subject to restriction by the hydrostatic pressure and cubic LMs with heights of up to 5 mm, which is the maximum height limit for LMs stabilized with nano/micrometer‐sized particles, are fabricated. Reduction of the hydrostatic pressure by changing the LM shape from cube to pyramid and introduction of particle‐stabilized bubble into the LM enabled the increase of height of the LM up to 9.8 mm, the highest height ever reported. Investigation using non‐aqueous liquids as an inner liquid confirmed that the longer the capillary length, the higher the maximum possible height of LMs. Finally, the polyhedral LMs are demonstrated to function as a microreactor for silver mirror reaction and chemical oxidative polymerization, resulting in the formation of Janus polymer plates.

show abstract

Open millifluidics based on powder-encased channels

Cited by 3 publications

References 32 publications

Rod‐Shaped Liquid Plasticine as Cuttable Minireactor for Photodynamic Therapy of Tumors

Rod‐Shaped Liquid Plasticine as Cuttable Minireactor for Photodynamic Therapy of Tumors

Quantitative Analysis of a Pilot Transwell Barrier Model with Automated Sampling and Mathematical Modeling

How Wide and High can Polyhedral Liquid Marbles be Fabricated?

Contact Info

Product

Resources

About