Open fermentative production of l-lactic acid with high optical purity by thermophilic Bacillus coagulans using excess sludge as nutrient

Ma, Kedong; Maeda, Takeyasu; Huh, You; Shirai, Yoshihito

doi:10.1016/j.biortech.2013.10.022

Cited by 85 publications

(62 citation statements)

References 21 publications

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“…Therefore, for the rest of the experiments, glucose as a carbon source was added to the culture medium and the ADS served as a source of nutrients other than carbon. This result was in accordance with other works of literature (Ma et al 2014;Kobayashi et al 2005). In addition to this, in order to convey the significance of ADS on ethanol yield, S. cerevisiae was inoculated to a sludge-free medium, consisted of only glucose and water under anaerobic condition.…”

Section: Effect Of Pretreatment Of Ads On the Ethanol Yield Of S Cersupporting

confidence: 91%

“…Thus, biotechnological products such as biopesticides (Yezza et al 2005), biochemicals such as lactic acid (Ma et al 2014), and biofuels such as biohydrogen (Ting and Lee 2007) have been produced using WAS as a nutrient source for the involved microorganisms. Cheung and Anderson (1997) tried to produce ethanol from the cellulose content of primary sludge of WWTPs, via simultaneous saccharification and fermentation (SSF).…”

Section: Introductionmentioning

confidence: 99%

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Anaerobic digested sludge: a new supplementary nutrient source for ethanol production

Bashiri

Farhadian

Asadollahi

et al. 2016

Int. J. Environ. Sci. Technol.

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Effluent sludge from an anaerobic digester was used as a source of nitrogen, phosphate, sulfur, and other nutrients in the culture medium of ethanol production by the yeast Saccharomyces cerevisiae. Several pretreatments (mechanical, chemical, thermal, and thermo-chemical) were performed on the anaerobic digested sludge (ADS) to make the nutrients accessible to the yeast cells. Preliminary experiments revealed that S. cerevisiae is not able to assimilate the carbon content of the ADS. However, when glucose was added to the medium, ethanol production was observed. The yield of ethanol using untreated ADS was only 10 % of the theoretical yield, but alkaline pretreatment improved it up to 43 %. By separating the hydrolysate of alkaline-treated ADS from the suspended solids, the ethanol yield from the supernatant was further improved up to 65 % of theoretical yield. Alkaline-treated ADS exhibited competitive performance with the mixture of yeast extract and mineral salts in ethanol fermentation.

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Section: Effect Of Pretreatment Of Ads On the Ethanol Yield Of S Cersupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Anaerobic digested sludge: a new supplementary nutrient source for ethanol production

Bashiri

Farhadian

Asadollahi

et al. 2016

Int. J. Environ. Sci. Technol.

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“…They potentially can be used as platform organisms if they are genetically accessible. A number of facultatively anaerobic thermophilic hosts have been studied for green chemical and fuel production (reviewed in references 7 and 8), such as Bacillus coagulans for lactic acid (9,10), Bacillus licheniformis for 2,3-butanediol (11,12), and Geobacillus thermoglucosidans for ethanol production (13). Examples of strictly anaerobic thermophiles that have been studied for biofuel production are Clostridium thermocellum (14,15), several Caldicellulosiruptor spp.…”

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confidence: 99%

Isolation and Screening of Thermophilic Bacilli from Compost for Electrotransformation and Fermentation: Characterization of Bacillus smithii ET 138 as a New Biocatalyst

Bosma

Weijer

Daas

et al. 2015

Appl Environ Microbiol

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b Thermophilic bacteria are regarded as attractive production organisms for cost-efficient conversion of renewable resources to green chemicals, but their genetic accessibility is a major bottleneck in developing them into versatile platform organisms. In this study, we aimed to isolate thermophilic, facultatively anaerobic bacilli that are genetically accessible and have potential as platform organisms. From compost, we isolated 267 strains that produced acids from C 5 and C 6 sugars at temperatures of 55°C or 65°C. Subsequently, 44 strains that showed the highest production of acids were screened for genetic accessibility by electroporation. Two Geobacillus thermodenitrificans isolates and one Bacillus smithii isolate were found to be transformable with plasmid pNW33n. Of these, B. smithii ET 138 was the best-performing strain in laboratory-scale fermentations and was capable of producing organic acids from glucose as well as from xylose. It is an acidotolerant strain able to produce organic acids until a lower limit of approximately pH 4.5. As genetic accessibility of B. smithii had not been described previously, six other B. smithii strains from the DSMZ culture collection were tested for electroporation efficiencies, and we found the type strain DSM 4216 T and strain DSM 460 to be transformable. The transformation protocol for B. smithii isolate ET 138 was optimized to obtain approximately 5 ؋ 10 3 colonies per g plasmid pNW33n. Genetic accessibility combined with robust acid production capacities on C 5 and C 6 sugars at a relatively broad pH range make B. smithii ET 138 an attractive biocatalyst for the production of lactic acid and potentially other green chemicals. Green chemicals are sustainable bio-based alternatives for chemicals based on fossil resources. Biomass is considered an attractive renewable resource for the production of such green chemicals. Major challenges for using biomass are to develop costeffective production processes and to convert substrates that go beyond first-generation pure sugars. From this perspective, the use of microbial fermentation processes that convert lignocellulosic sugars is gaining considerable attention. For particular products natural producers can be used, but often Escherichia coli and Saccharomyces cerevisiae are used as platform organisms because of their well-known physiology and ease of engineering for the production of many different products (1, 2).In general, most organisms used for the production of green chemicals and fuels are mesophiles, and current processes still are based mainly on first-generation feedstocks, i.e., sucrose from sugar beet or sugarcane or glucose derived from starches from corn or tapioca. Although the engineering of platform organisms broadens the spectrum of possible substrates (3), the efficiency of the process could be increased by the use of organisms naturally capable of degrading lignocellulose-derived sugars (4). To further increase the efficiency and reduce the costs of the microbial production of green chemicals, modera...

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“…Lei reported that the dietary inclusion of spores of a lactobacillus improved the conversion ratio and reduced mortality and culling rate of broilers (Lei et al, 2015). Lactobacilli improve calcium and phosphorus utilization, and lactic acid promotes the transformation of pepsinogen into protease, stimulating the peristalsis of the small intestine to enhance nutrient digestion (Ma et al, 2014;Giang et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Bacillus Coagulans Enhance the Immune Function of the Intestinal Mucosa of Yellow Broilers

Fan

Zhuang

et al. 2017

Rev. Bras. Cienc. Avic.

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This experiment was conducted to investigate the effects of Bacillus coagulans on the growth performance and immune functions of the intestinal mucosa of yellow broilers. Three hundred and sixty one-day-old yellow chicks were randomly allocated to four treatments groups with six replicates of 15 chicks each. The broilers were randomly subjected to one of the following treatments for 28 days: control group (group1, fed a basal diet) and three treatments (group 2, 3, 4) fed the basal diet supplemented with 100, 200, or 300 mg/kg Bacillus coagulans, respectively). The results showed that for 28 days, compared with the control diet, the dietary addition of 200 mg/kg Bacillus coagulans significantly decreased the feed/gain ratio (F/G) (p<0.05), improved the thymus index, spleen index and bursa index (p<0.05), increased the villus height to crypt depth ratio (V/C) in the duodenum (p<0.05), increased the number of secretory immunoglobulin (sIgA) positive cells (p<0.05). The dietary addition of 200 mg/kg Bacillus coagulans promoted a significant increase in Lactobacillus spp. populations and suppressed Escherichia coli replication in cecum, compared with the control (p<0.05). Moreover, the dietary addition of 200 mg/kg Bacillus coagulans also significantly enhanced the levels of interferon alpha (IFNα), toll-like receptor (TLR3), and melanoma differentiationassociated protein 5(MDA5) in the duodenum (p<0.05). In conclusion, the dietary addition of Bacillus coagulans significantly improved broiler performance, and enhanced the intestinal mucosal barrier and immune function. The optimal dosage of Bacillus coagulans for yellow broilers was determined as 2×10 8 cfu/kg.

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Open fermentative production of l-lactic acid with high optical purity by thermophilic Bacillus coagulans using excess sludge as nutrient

Cited by 85 publications

References 21 publications

Anaerobic digested sludge: a new supplementary nutrient source for ethanol production

Anaerobic digested sludge: a new supplementary nutrient source for ethanol production

Isolation and Screening of Thermophilic Bacilli from Compost for Electrotransformation and Fermentation: Characterization of Bacillus smithii ET 138 as a New Biocatalyst

Bacillus Coagulans Enhance the Immune Function of the Intestinal Mucosa of Yellow Broilers

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