OoNAC72, a NAC-Type Oxytropis ochrocephala Transcription Factor, Conferring Enhanced Drought and Salt Stress Tolerance in Arabidopsis

Guan, Hongwei; Liu, Xin; Niu, Fei; Zhao, Qianqian; Fan, Ni; Cao, Deliang; Meng, Dian; He, Wei; Guo, Bin; Wei, Yahui; Fù, Yànpíng

doi:10.3389/fpls.2019.00890

Cited by 36 publications

(14 citation statements)

References 65 publications

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“…We selected lines 27# and 28# for further investigations based on the screening results on 0.5 µM ABA and expression analysis ( Figure S2) of randomly selected 5 transgene linesfrom T3 generations. Our selection of two lines for further studies is consistent with Huang et al [46], Guan et al [47], and Shi et al [48]. ABA act as an important phytohormone in the plant responses to abiotic stresses [49], and several studies have shown that ABA sensitivity correlate with stress tolerance in plants [50][51][52].…”

Section: Discussionsupporting

confidence: 88%

CaDHN4, a Salt and Cold Stress-Responsive Dehydrin Gene from Pepper Decreases Abscisic Acid Sensitivity in Arabidopsis

Zhang

Liu

et al. 2019

IJMS

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Dehydrins play an important role in improving plant resistance to abiotic stresses. In this study, we isolated a dehydrin gene from pepper (Capsicum annuum L.) leaves, designated as CaDHN4. Sub-cellular localization of CaDHN4 was to be found in the nucleus and membrane. To authenticate the function of CaDHN4 in cold- and salt-stress responses and abscisic acid (ABA) sensitivity, we reduced the CaDHN4 expression using virus-induced gene silencing (VIGS), and overexpressed the CaDHN4 in Arabidopsis. We found that silencing of CaDHN4 reduced the growth of pepper seedlings and CaDHN4-silenced plants exhibited more serious wilting, higher electrolyte leakage, and more accumulation of ROS in the leaves compared to pTRV2:00 plants after cold stress, and lower chlorophyll contents and higher electrolyte leakage compared to pTRV2:00 plants under salt stress. However, CaDHN4-overexpressing Arabidopsis plants had higher seed germination rates and post-germination primary root growth, compared to WT plants under salt stress. In response to cold and salt stresses, the CaDHN4-overexpressed Arabidopsis exhibited lower MDA content, and lower relative electrolyte leakage compared to the WT plants. Under ABA treatments, the fresh weight and germination rates of transgenic plants were higher than WT plants. The transgenic Arabidopsis expressing a CaDHN4 promoter displayed a more intense GUS staining than the normal growth conditions under treatment with hormones including ABA, methyl jasmonate (MeJA), and salicylic acid (SA). Our results suggest that CaDHN4 can protect against cold and salt stresses and decrease ABA sensitivity in Arabidopsis.

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Section: Discussionsupporting

confidence: 88%

CaDHN4, a Salt and Cold Stress-Responsive Dehydrin Gene from Pepper Decreases Abscisic Acid Sensitivity in Arabidopsis

Zhang

Liu

et al. 2019

IJMS

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“…TFs are critical regulatory factors for developmental processes and stress responses in plant. TFs bHLH, MYB, NAC participated in regulating seed germination in rice, wheat, and Arabidopsis, respectively (Chen et al, 2018;Zhao et al, 2018;Guan et al, 2019). Four DEGs encoding ubiquitin proteasome system proteins (GeneID: 108332901, 108337045, 108319922, 108329730), and five TFs (GeneID: 108331170, NC_030637.1.548, 108331246, 108338278, 108346333) were identified, indicating they might regulate seed germination in response to drought stress in adzuki bean.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Drought-Responsive Transcriptome During Seed Germination in Adzuki Bean (Vigna angularis L.) by PacBio SMRT and Illumina Sequencing

et al. 2020

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The full-length single-molecular sequencing and short reads Illumina sequencing were combined to generate the transcripts of adzuki bean with high-quality. A total of 17,636 loci and 60,454 transcripts were detected in this study. To characterize the drought-responsive genes during seed germination in adzuki bean, two varieties, i.e., tolerant and sensitive to drought stress, were selected to conduct analysis of alternative splicing dynamics (AS) and differentially expressed genes (DEGs) by combining the newly assembled draft genome and public adzuki bean reference genome. AS analysis indicated that both the two varieties underwent a little more AS events under control conditions than under drought stress. Among the AS events, IR (intron retention) predominately accounted for 34.3%, whereas AD (alternative donor site) was the least frequent with 15.8%. Meanwhile, 562 long non-coding RNAs, 409 fusion genes and 1208 transcription factors were identified. Moreover, a total of 5,337 DEGs were identified in comparison of the two varieties with drought or control treatments. Notably, 82 DEGs were discovered in the two varieties under drought stress, which might be the candidate in regulation of seed germination to answer for different drought tolerance. The DEGs encoded proteins involved in primary or second metabolism, plant hormone signal transduction, transcript or translation processes, ubiquitin proteasome system, transcription factor, transporters, and so on. The results facilitate to increase the knowledge about the mechanism of drought tolerance during crop seed germination, and provide reference for the breeding of drought-tolerant adzuki bean.

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“…Being able to regulate gene expression, transcription factors (TFs) have been known to be indispensable players in the regulation of plant drought responses [16,17]. Among the drought-related TF families in plants, the NAC (NAM—No Apical Meristem of petunia, ATAF1/2— Arabidopsis Transcription-Activating Factor1/2, and CUC2—Cup-Shaped Cotyledon2) family has been identified as a key plant-specific TF family [18,19,20,21]. The NAC proteins are characterized by the possession of a conserved DNA-binding domain at the N-terminus and a transcriptional regulatory domain at the C-terminus [22].…”

Section: Introductionmentioning

confidence: 99%

Ectopic Expression of Glycine max GmNAC109 Enhances Drought Tolerance and ABA Sensitivity in Arabidopsis

et al. 2019

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The NAC (NAM, ATAF1/2, CUC2) transcription factors are widely known for their various functions in plant development and stress tolerance. Previous studies have demonstrated that genetic engineering can be applied to enhance drought tolerance via overexpression/ectopic expression of NAC genes. In the present study, the dehydration- and drought-inducible GmNAC109 from Glycine max was ectopically expressed in Arabidopsis (GmNAC109-EX) plants to study its biological functions in mediating plant adaptation to water deficit conditions. Results revealed an improved drought tolerance in the transgenic plants, which displayed greater recovery rates by 20% to 54% than did the wild-type plants. In support of this finding, GmNAC109-EX plants exhibited lower water loss rates and decreased endogenous hydrogen peroxide production in leaf tissues under drought, as well as higher sensitivity to exogenous abscisic acid (ABA) treatment at germination and early seedling development stages. In addition, analyses of antioxidant enzymes indicated that GmNAC109-EX plants possessed stronger activities of superoxide dismutase and catalase under drought stress. These results together demonstrated that GmNAC109 acts as a positive transcriptional regulator in the ABA-signaling pathway, enabling plants to cope with adverse water deficit conditions.

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OoNAC72, a NAC-Type Oxytropis ochrocephala Transcription Factor, Conferring Enhanced Drought and Salt Stress Tolerance in Arabidopsis

Cited by 36 publications

References 65 publications

CaDHN4, a Salt and Cold Stress-Responsive Dehydrin Gene from Pepper Decreases Abscisic Acid Sensitivity in Arabidopsis

CaDHN4, a Salt and Cold Stress-Responsive Dehydrin Gene from Pepper Decreases Abscisic Acid Sensitivity in Arabidopsis

Characterization of Drought-Responsive Transcriptome During Seed Germination in Adzuki Bean (Vigna angularis L.) by PacBio SMRT and Illumina Sequencing

Ectopic Expression of Glycine max GmNAC109 Enhances Drought Tolerance and ABA Sensitivity in Arabidopsis

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