Oogonial biometry and phylogenetic analyses of the Pythium vexans species group from woody agricultural hosts in South Africa reveal distinct groups within this taxon

Spies, Chris; Mazzola, Mark; Botha, W. J.; Rijst, Marieta van der; Mostert, Lizel; McLeod, A.

doi:10.1016/j.funbio.2010.11.005

Cited by 25 publications

(15 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several other species are indistinguishable even when both ITS and cox1 sequences are compared (see Text S1B of Robideau et al 2011), and many of these should probably be formally synonymized pending more thorough investigations with multiple hypervariable genes. This approach should also resolve species complexes found in the group formed by P. irregulare, P. paroecandrum, P. cylindrosporum, P. cryptoirregulare and P. mamillatum (Barr et al 1997;Matsumoto et al 2000;Garzón et al 2007;Spies et al 2011a), the varieties of P. ultimum (Barr et al 1996), and the P. vexans and P. cucurbitacearum group (Spies et al 2011b). Some species epithets have been applied to multiple phylogenetic species due to imprecise species descriptions and/or misidentifications.…”

Section: Species Identification and Numbersmentioning

confidence: 99%

One stop shop: backbones trees for important phytopathogenic genera: I (2014)

et al. 2014

View full text Add to dashboard Cite

Many fungi are pathogenic on plants and cause significant damage in agriculture and forestry. They are also part of the natural ecosystem and may play a role in regulating plant numbers/density. Morphological identification and analysis of plant pathogenic fungi, while important, is often hampered by the scarcity of discriminatory taxonomic characters and the endophytic or inconspicuous nature of these fungi. Molecular (DNA sequence) data for plant pathogenic fungi have emerged as key information for diagnostic and classification studies, although hampered in part by non-standard laboratory practices and analytical methods. To facilitate current and future research, this study provides phylogenetic synopses for 25 groups of plant pathogenic fungi in the Ascomycota, Basidiomycota, Mucormycotina (Fungi), and Oomycota, using recent molecular data, up-to-date names, and the latest taxonomic insights. Lineagespecific laboratory protocols together with advice on their application, as well as general observations, are also provided. We hope to maintain updated backbone trees of these fungal lineages over time and to publish them jointly as new data emerge. Researchers of plant pathogenic fungi not covered by the present study are invited to join this future effort. Bipolaris, Botryosphaeriaceae,

show abstract

Section: Species Identification and Numbersmentioning

confidence: 99%

One stop shop: backbones trees for important phytopathogenic genera: I (2014)

et al. 2014

View full text Add to dashboard Cite

show abstract

“…A similar finding was made for one isolate (13e57) in the study of Garz on et al (2007). Incongruency in nuclear and mitochondrial data sets has recently been reported for the Pythium vexans species group (Spies et al 2011b) and also in Phytophthora (Martin & Tooley 2003;Kroon et al 2004). Incongruency between these data sets may suggest that (i) the mitochondrial genome has a different rate of evolutionary divergence than the nuclear genome (Martin & Tooley 2003) or (ii) sexual outcrossing or hybridisation has occurred (Martin & Tooley 2003;Kroon et al 2004) since mitochondrial sequences are inherited uniparentally (F€ orster & Coffey 1990).…”

Section: Discussionmentioning

confidence: 96%

“…The primers used for PCR amplification of each region are listed in Table 2. PCR amplification and sequencing of these regions were performed as described previously (Spies et al 2011b). …”

Section: Dna Extraction Polymerase Chain Reaction (Pcr) Amplificatiomentioning

confidence: 99%

See 1 more Smart Citation

Molecular analyses of Pythium irregulare isolates from grapevines in South Africa suggest a single variable species

Spies

Mazzola

Botha³

et al. 2011

Fungal Biology

Self Cite

View full text Add to dashboard Cite

“…Three loci (ITS, COX1, btubulin) were chosen for the characterization of the isolates. PCR amplification of the ITS region was done according to Drenth et al (2006) and amplification of the COX1 and ß-tubulin regions and sequencing of all regions were performed as described by Spies et al (2011), with the exception that primers I2 and A2 (Drenth et al 2006) were used for sequencing of the ITS region.…”

Section: Plugs In Watermentioning

confidence: 99%

Two clonal lineages of Phytophthora citrophthora from citrus in South Africa represent a single phylogenetic species

et al. 2014

Self Cite

View full text Add to dashboard Cite

Phytophthora citrophthora from citrus in eastern Corsica and Spain consists of distinct clonal lineages. In South Africa the extent of genetic variation among citrus-associated P. citrophthora isolates is unknown. This was investigated with isolates from South Africa (n =60), Spain (n =10) and six isolates representing three P. citrophthora groups CTR1, CTR2 and CTR3 previously identified with isozyme polymorphisms (Mchau and Coffey 1994). South African and Spanish isolates belonged to two lineages (G1, G2) based on an internal transcribed spacer (ITS) phylogeny, random amplified microsatellites (RAMS) and random amplified polymorphic DNA (RAPD) profiling. Although combined RAMS and RAPD data identified 14 genotypes, unweighted pair group method with arithmetic mean (UPGMA) analyses grouped the isolates into two clusters corresponding to lineages G1 and G2. Lineage G1 predominated among isolates from South Africa (92%) and Spain (100%). Phylogenetic analyses of the β-tubulin, cytochrome c oxidase subunit I (COX1) and ITS regions did not support the hypothesis that the two lineages represent distinct phylogenetic species but suggested that isozyme group CTR2 and possibly CTR3 are species distinct from P. citrophthora sensu stricto. Mating-type analyses, using tester strains from groups CTR2 and CTR3 revealed that most G1 lineage isolates (n =57) were sterile but that some were of the A1 mating type (n =8) whereas all G2 lineage isolates were A2 (n =5). The mating-type designation was confirmed with P. capsici tester strains. However, when A1 (G1 lineage) and A2 (G2 lineage including CTR1 reference isolates) mating-type isolates were paired in all possible combinations, no oogonia or antheridia were produced. This suggests that only tester strains P. capsici, CTR2 and CTR3 were able to produce sexual structures and that lineages G1 and G2 are sterile and reproductively isolated, which is supported by molecular data.

show abstract

Oogonial biometry and phylogenetic analyses of the Pythium vexans species group from woody agricultural hosts in South Africa reveal distinct groups within this taxon

Cited by 25 publications

References 31 publications

One stop shop: backbones trees for important phytopathogenic genera: I (2014)

One stop shop: backbones trees for important phytopathogenic genera: I (2014)

Molecular analyses of Pythium irregulare isolates from grapevines in South Africa suggest a single variable species

Two clonal lineages of Phytophthora citrophthora from citrus in South Africa represent a single phylogenetic species

Contact Info

Product

Resources

About