Oocytes suppress FOXL2 expression in cumulus cells in mice†

Emori, Chihiro; Ito, Haruka; Fujii, Wataru; Naito, Kunihiko; Sugiura, Koji

doi:10.1093/biolre/ioaa054

Cited by 13 publications

(25 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The expression of mouse anti-human ACTIN polyclonal antibody (Servicebio; diluted 1:3000) was used as an internal reference. A shows the protein expression of ABCA1, LDLR, SCARB1, SULT2B1, and CYP19A1; B shows the protein expression of CRLS1, LPCAT3, and PLA2G4A initial stage, they play different roles during follicle development [10]. It has been reported that there were significant functional differences in both CCs involved in steroidogenesis and MGCs involved in angiogenesis compartments in human follicles [38].…”

Section: Discussionmentioning

confidence: 99%

“…Usually, people regard MGCs and CCs as the same cells. However, though both CCs and MGCs seem to alike at the initial stage, they play different roles during follicle development [ 10 ]. It has been reported that there were significant functional differences in both CCs involved in steroidogenesis and MGCs involved in angiogenesis compartments in human follicles [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

“…During follicle development, CCs and MGCs play different roles, and the normal development of these cell lineages is essential for female fertility. The intracellular mechanism of dividing CCs and MGCs under the control of oocytes has not yet been fully resolved [ 10 ]. In this study, we aimed to characterize the differences between human CCs and MGCs, especially their metabolomics differences.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Differential Metabolomes in Cumulus and Mural Granulosa Cells from Human Preovulatory Follicles

et al. 2021

View full text Add to dashboard Cite

This study evaluated the differences in metabolites between cumulus cells (CCs) and mural granulosa cells (MGCs) from human preovulatory follicles to understand the mechanism of oocyte maturation involving CCs and MGCs. CCs and MGCs were collected from women who were undergoing in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) treatment. The differences in morphology were determined by immunofluorescence. The metabolomics of CCs and MGCs was measured by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) followed by quantitative polymerase chain reaction (qPCR) and western blot analysis to further confirm the genes and proteins involved in oocyte maturation. CCs and MGCs were cultured for 48 h in vitro, and the medium was collected for detection of hormone levels. There were minor morphological differences between CCs and MGCs. LC-MS/MS analysis showed that there were differences in 101 metabolites between CCs and MGCs: 7 metabolites were upregulated in CCs, and 94 metabolites were upregulated in MGCs. The metabolites related to cholesterol transport and estradiol production were enriched in CCs, while metabolites related to antiapoptosis were enriched in MGCs. The expression of genes and proteins involved in cholesterol transport (ABCA1, LDLR, and SCARB1) and estradiol production (SULT2B1 and CYP19A1) was significantly higher in CCs, and the expression of genes and proteins involved in antiapoptosis (CRLS1, LPCAT3, and PLA2G4A) was significantly higher in MGCs. The level of estrogen in CCs was significantly higher than that in MGCs, while the progesterone level showed no significant differences. There are differences between the metabolomes of CCs and MGCs. These differences may be involved in the regulation of oocyte maturation.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Differential Metabolomes in Cumulus and Mural Granulosa Cells from Human Preovulatory Follicles

et al. 2021

View full text Add to dashboard Cite

show abstract

“…This is followed by the dramatic growth of oocytes [2]. Subsequently, GCs rapidly proliferate to form an antral structure composed of multilayer granulosa cells and differentiate into mural GCs and cumulus GCs [3,4]. Only a subset of the antral follicles responds to LH and further releases fertilizable oocytes.…”

Section: Introductionmentioning

confidence: 99%

miR-135a Suppresses Granulosa Cell Growth by Targeting Tgfbr1 and Ccnd2 during Folliculogenesis in Mice

Wang

Chen

Tang

et al. 2021

Cells

View full text Add to dashboard Cite

The success of female reproduction relies on high quality oocytes, which is determined by well-organized cooperation between granulosa cells (GCs) and oocytes during folliculogenesis. GC growth plays a crucial role in maintaining follicle development. Herein, miR-135a was identified as a differentially expressed microRNA in pre-ovulatory ovarian follicles between Large White and Chinese Taihu sows detected by Solexa deep sequencing. We found that miR-135a could significantly facilitate the accumulation of cells arrested at the G1/S phase boundary and increase apoptosis. Mechanically, miR-135a suppressed transforming growth factor, beta receptor I (Tgfbr1) and cyclin D2 (Ccnd2) expression by targeting their 3′UTR in GCs. Furthermore, subcellular localization analysis and a chromatin immunoprecipitation-quantitative real-time PCR (ChIP-qPCR) assay demonstrated that the TGFBR1-SMAD3 pathway could enhance Ccnd2 promoter activity and thus upregulate Ccnd2 expression. Finally, estrogen receptor 2 (ESR2) functioned as a transcription factor by directly binding to the miR-135a promoter region and decreasing the transcriptional activity of miR-135a. Taken together, our study reveals a pro-survival mechanism of ESR2/miR-135a/Tgfbr1/Ccnd2 axis for GC growth, and also provides a novel target for the improvement of female fertility.

show abstract

“…Moreover, oocytes facilitate fibroblast growth factor (FGF) signaling in cumulus cells by suppressing the expression of the FGF receptor antagonist Spry2 [ 5 ]. Furthermore, they control the expression of transcriptional regulators, such as transcription factors and their coregulators, in granulosa cells to determine the cumulus cell lineage [ 6 ]. This suggests that oocytes regulate the expression of signal receptors and key transcription factors, as well as their coregulators, to coordinate intra- and extra-follicular signals in cumulus cells.…”

mentioning

confidence: 99%

Expression and regulation of estrogen receptor 2 and its coregulators in mouse granulosa cells

Emori

Kanke

Ito

et al. 2022

Journal of Reproduction and Development

Self Cite

View full text Add to dashboard Cite

The cooperative effects of estrogen and oocyte-derived paracrine factors (ODPFs) play critical roles in the normal development of ovarian follicles; however, the mechanism underlying this cooperation has not been well studied. The present study aimed to determine whether ODPFs affect estrogen signaling by regulating the expression of estrogen receptor (ESR) and its coregulators in mouse granulosa cells. Some transcripts encoding ESR coregulators were differentially expressed between cumulus and mural granulosa cells (MGCs). The transcript levels of ESR coregulators, including nuclear receptor corepressor 1 and activator 2, in cumulus cells were significantly suppressed by ODPFs; however, they increased when cumulus cell-oocyte complexes were treated with the transforming growth factor beta receptor I inhibitor, SB431542. Moreover, MGCs exhibited significantly higher ESR2 protein and transcript levels than those in cumulus cells. ODPFs promoted Esr2 expression in cumulus cells but had no effect on that in MGCs. Overall, regulation of the expression of ESR2 and its coregulators in cumulus cells by oocytes seems to be one of the mechanisms underlying estrogen-oocyte cooperation in well-developed antral follicles in mice.

show abstract

Oocytes suppress FOXL2 expression in cumulus cells in mice†

Cited by 13 publications

References 54 publications

The Differential Metabolomes in Cumulus and Mural Granulosa Cells from Human Preovulatory Follicles

The Differential Metabolomes in Cumulus and Mural Granulosa Cells from Human Preovulatory Follicles

miR-135a Suppresses Granulosa Cell Growth by Targeting Tgfbr1 and Ccnd2 during Folliculogenesis in Mice

Expression and regulation of estrogen receptor 2 and its coregulators in mouse granulosa cells

Contact Info

Product

Resources

About