Oocyte vitrification does not increase the risk of embryonic aneuploidy or diminish the implantation potential of blastocysts created after intracytoplasmic sperm injection: a novel, paired randomized controlled trial using DNA fingerprinting

Forman, Eric J.; Li, Xinying; Ferry, K.M.; Scott, Katherine; Treff, Nathan R.; Scott, Richard T.

doi:10.1016/j.fertnstert.2012.04.028

Cited by 134 publications

(88 citation statements)

References 17 publications

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“…The information available on the neonatal outcome and the long-term follow-up of children conceived after oocyte cryopreservation is limited (Chian et al, 2008(Chian et al, , 2014Noyes et al, 2009;Wennerholm et al, 2009;Cobo et al, 2014) and only small studies have specifically addressed the safety of oocyte vitrification (Coticchio et al, 2009;Bonetti et al, 2011;Chen et al, 2012;Forman et al, 2012;Khalili et al, 2012;Monzo et al, 2012;Dominguez et al, 2013;Gugliemo et al, 2014;Konc et al, 2014;Nohales Có rcoles et al, 2014;Palmerini et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Chromosomal meiotic segregation, embryonic developmental kinetics and DNA (hydroxy)methylation analysis consolidate the safety of human oocyte vitrification

Munck

Petrussa

Verheyen³

et al. 2015

MHR: Basic Science of Reproductive Medicine

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Oocyte vitrification has been introduced into clinical settings without extensive pre-clinical safety testing. In this study, we analysed major safetyaspects of human oocyte vitrification in a high security closed system: (i) chromosomal meiotic segregation, (ii) embryonic developmental kinetics and (iii) DNA (hydroxy)methylation status. Fresh and vitrified sibling oocytes from young donors after intracytoplasmic sperm injection (ICSI) were compared in three different assays. Firstly, the chromosomal constitution of the fertilized zygotes was deduced from array comparative genomic hybridization results obtained from both polar bodies biopsied at Day 1. Secondly, embryo development up to Day 3 was analysed by time-lapse imaging. Ten specific time points, six morphokinetic time intervals and the average cell number on Day 3 were recorded. Thirdly, global DNA methylation and hydroxymethylation patterns were analysed by immunostaining on Day 3 embryos. The nuclear fluorescence intensity was measured by Volocity imaging software. Comprehensive chromosomal screening of the polar bodies demonstrated that at least half of the zygotes obtained after ICSI of fresh and vitrified oocytes were euploid. Time-lapse analysis showed that there was no significant difference in cleavage timings, the predictive morphokinetic time intervals nor the average cell number between embryos developed from fresh and vitrified oocytes. Finally, global DNA (hydroxy)methylation patterns were not significantly different between Day 3 embryos obtained from fresh and from vitrified oocytes. Our data further consolidate the safety of the oocyte vitrification technique. Nevertheless, additional testing in young and older sub-fertile/infertile patients and sound follow-up studies of children born after oocyte cryopreservation remain mandatory.

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Section: Introductionmentioning

confidence: 99%

Chromosomal meiotic segregation, embryonic developmental kinetics and DNA (hydroxy)methylation analysis consolidate the safety of human oocyte vitrification

Munck

Petrussa

Verheyen³

et al. 2015

MHR: Basic Science of Reproductive Medicine

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“…Furthermore, vitrification is considered to be a better alternative to slow freezing cryopreservation [31]. Regardless of the methodology used for cryopreservation, the pregnancy rate obtained with frozen/thawed gametes and embryos is still below that obtained with fresh gametes and embryos [32]. This finding suggests that improvements are still needed in oocyte and embryo cryopreservation.…”

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confidence: 99%

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

Moussa

Shen

Zhang

et al. 2014

Sci. China Life Sci.

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Cryopreservation techniques for mammalian oocytes and embryos have rapidly progressed during the past two decades, emphasizing their importance in various assisted reproductive technologies. Pregnancies and live births resulting from cryopreserved oocytes and embryos of several species including humans have provided proof of principle and led to the adoption of cryopreservation as an integral part of clinical in vitro fertilization. Considerable progress has been achieved in the development and application of the cryopreservation of mammalian oocytes and embryos, including preservation of the reproductive potential of patients who may become infertile, establishment of cryopreserved oocyte banks, and transport of oocytes and embryos internationally. However, the success rates are still far lower than those obtained with fresh oocytes and embryos, and there are still obstacles that need to be overcome. In this review, we address the major obstacles in the development of effective cryopreservation techniques. Such knowledge may help to eliminate these hurdles by revealing which aspects need improvement. Furthermore, this information may encourage further research by cryobiologists and increase the practical use of cryopreservation as a major part of assisted reproductive technologies for both humans and animal species. cryopreservation, mammals, oocytes, embryos, cryoinjuries Citation:Moussa M, Shu J, Zhang XH, Zeng FY. Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives. Sci China Life Sci, 2014, 57: 903 -914,

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“…However, aneuploidy rates have not been shown to increase following oocyte vitrification (Forman et al, 2012;Goldman et al, 2015). Second, oocytes from one donor cycle may have been used for multiple recipients but because this was not specified in the database, in the analyses, each cycle was treated as an independent cycle.…”

Section: Analysis Of Samplesmentioning

confidence: 99%

Euploidy Rates in Donor Egg Cycles Significantly Differ Between Fertility Centers

Munné

Alikani

Colls

et al. 2017

Obstetrical &Amp; Gynecological Survey

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Oocyte vitrification does not increase the risk of embryonic aneuploidy or diminish the implantation potential of blastocysts created after intracytoplasmic sperm injection: a novel, paired randomized controlled trial using DNA fingerprinting

Cited by 134 publications

References 17 publications

Chromosomal meiotic segregation, embryonic developmental kinetics and DNA (hydroxy)methylation analysis consolidate the safety of human oocyte vitrification

Chromosomal meiotic segregation, embryonic developmental kinetics and DNA (hydroxy)methylation analysis consolidate the safety of human oocyte vitrification

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

Euploidy Rates in Donor Egg Cycles Significantly Differ Between Fertility Centers

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