Oocyte vitrification as an efficient option for elective fertility preservation

Cobo, Ana; García-Velasco, Juan A.; Coello, Aila; Domingo, Javier; Pellicer, António; Remohı́, José

doi:10.1016/j.fertnstert.2015.11.027

Cited by 439 publications

(329 citation statements)

References 26 publications

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“…It is evident from current work on de novo mutations that advising the public, including policy makers, on potential risks of advanced paternal age and the burden it might bring on society is crucial. An extreme “solution” if reproduction is to be postponed might be to promote cryopreservation of oocytes and sperm [197], a measure under much debate that has been termed “social freezing”.…”

Section: De Novo Mutations In Clinical Practicementioning

confidence: 99%

New insights into the generation and role of de novo mutations in health and disease

2016

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Aside from inheriting half of the genome of each of our parents, we are born with a small number of novel mutations that occurred during gametogenesis and postzygotically. Recent genome and exome sequencing studies of parent–offspring trios have provided the first insights into the number and distribution of these de novo mutations in health and disease, pointing to risk factors that increase their number in the offspring. De novo mutations have been shown to be a major cause of severe early-onset genetic disorders such as intellectual disability, autism spectrum disorder, and other developmental diseases. In fact, the occurrence of novel mutations in each generation explains why these reproductively lethal disorders continue to occur in our population. Recent studies have also shown that de novo mutations are predominantly of paternal origin and that their number increases with advanced paternal age. Here, we review the recent literature on de novo mutations, covering their detection, biological characterization, and medical impact.

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Section: De Novo Mutations In Clinical Practicementioning

confidence: 99%

New insights into the generation and role of de novo mutations in health and disease

2016

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“…Cryopreservation of oocytes, is standard practice in Assisted Reproductive Technologies123 (ART). Indeed, the experimental connotation, once associated with oocyte cryopreservation has been removed45.…”

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confidence: 99%

Glycine increases preimplantation development of mouse oocytes following vitrification at the germinal vesicle stage

Cao

Rose

Wang

et al. 2016

Sci Rep

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Ice-free cryopreservation, referred to as vitrification, is receiving increased attention in the human and animal assisted reproduction. However, it introduces the detrimental osmotic stress by adding and removing high contents of cryoprotectants. In this study, we evaluated the effects of normalizing cell volume regulation by adding glycine, an organic osmolyte, during vitrification of mouse germinal vesicle stage oocyte and/or subsequent maturation on its development. The data showed that glycine supplementation in either vitrification/thawing or maturation medium significantly improved the cytoplasmic maturation of MII oocytes manifested by spindle assembly, chromosomal alignment, mitochondrial distribution, euploidy rate, and blastocyst development following fertilization in vitro, compared to the control without glycine treatment. Furthermore, glycine addition during both vitrification/thawing and maturation further enhanced the oocyte quality demonstrated by various markers, including ATP contents and embryo development. Lastly, the effect of anti-apoptosis was also observed when glycine was added during vitrification. Our result suggests that reducing osmotic stress induced by vitrification could improve the development of vitrified mouse oocyte.

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“…This phenomenon was recently documented in a 137 and 368 autologous egg banking cycles in Spain and the USA, respectively, with 12-21.2% of the fertilized oocyte warming cycle failing to yield an embryo for transfer. 104,121 It is to be kept in mind that survival rates and normal fertilization events tend to be consistent (75-95% and 65-85%, respectively), with survival failure being a rare event (<2%). 105 Perhaps the greatest concern then is that naive, innocent female consumers (ie, perspective patients) are being misinformed about their risks, by financially motivated programs, that the freeze preservation of their potential fertility based on today's technology does not guarantee that a transferrable embryo will even result, let alone potentially create a healthy baby.…”

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confidence: 94%

Vitrification: the pioneering past to current trends and perspectives of cryopreserving human embryos, gametes and reproductive tissue

Schiewe¹,

Anderson²

2017

BSAM

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After more than 2 decades of development in mammalian models and the clinical focus of a few pioneering laboratories, vitrification of human oocytes and embryos has transformed today's assisted reproductive technology (ART) industry. Our ability to cryopreserve gametes and embryos without fear of the damaging effects of ice formation has instilled great confidence in post-warming specimen viability. In turn, clinical treatment options are progressively eliminating fresh embryo transfer (ET; ie, freeze-all cycles), integrating preimplantation genetic screening and contemplating the use of cryopreserved oocytes as a viable resource. Vitrification's impact on clinical treatments is akin to the advent of sperm injection technology in the 1990s on male factor infertility. An appreciable and quantifiable difference was made, with procedural efficacy and global reliability essentially being guaranteed. Yet, there are challenges in the current trends and perspectives in how this technology is and will be optimized in the future. User variation in vitrification products and procedures warrants stricter adherence to quality control measures to enhance specimen biosafety and patient satisfaction, while reducing potential liability concerns. Furthermore, future progress in our understanding of the chemical and cryophysical processes of vitrification will insure the effective cryostorage of reproductive tissues and gametes at a level attained for embryo cryopreservation today.

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Oocyte vitrification as an efficient option for elective fertility preservation

Cited by 439 publications

References 26 publications

New insights into the generation and role of de novo mutations in health and disease

New insights into the generation and role of de novo mutations in health and disease

Glycine increases preimplantation development of mouse oocytes following vitrification at the germinal vesicle stage

Vitrification: the pioneering past to current trends and perspectives of cryopreserving human embryos, gametes and reproductive tissue

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