Oocyte Maturation During Hormone-Induced Spawning in Largemouth Bass,<i>Micropterus salmoides</i>

Porter, Michael D.

doi:10.1300/j028v07n01_02

Cited by 3 publications

(4 citation statements)

References 18 publications

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“…Investigators have utilized a variety of agents to induce ovulation in LMB. The control treatment in this study, a single injection of HCG at 4,000 IU/kg BW, has been shown to be effective in inducing ovulation in LMB with a latent period of 36 to 40 h (Beaty 1976; Garrett et al 1992; Mayes et al, 1993; Porter 1997; Fries et al 2002; Gomelsky et al 2004; Stilwell 2008). Neal (2014) utilized Ovaprim at an initial priming dose of 0.1 mL/kg BW and a resolving dose of 0.4 mL/kg BW 8 h after the priming dose to produce triploid fish.…”

Section: Resultsmentioning

confidence: 90%

“…Neal (2014) utilized Ovaprim at an initial priming dose of 0.1 mL/kg BW and a resolving dose of 0.4 mL/kg BW 8 h after the priming dose to produce triploid fish. Luteinizing hormone releasing hormone analog (LHRHa), either as single injection or two injections over time (priming and resolving dose), has also been effective in stimulating egg release in LMB (Mayes et al, 1993; Porter 1997; Neal et al 2004; Stilwell 2008). These studies do not report the response rate and other production parameters.…”

Section: Resultsmentioning

confidence: 99%

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Evaluation of Hormonal Agents for Artificial Propagation of Largemouth Bass

2021

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A synthetic gonadotropin‐releasing hormone, cGnRH IIa (D‐Arg6, Pro9‐NHet), was evaluated versus commercially available hormones as a spawning aid for Largemouth Bass Micropterus salmoides with two trials each season for two seasons. In season one, human chorionic gonadotropin (HCG) was applied at a rate of 4,000 IU/kg for females and 2,000 IU/kg for males. Ovaprim (Western Chemical, Ferndale, Washington) and cGnRH IIa was applied at 0.5 mL/kg and 0.1 mg/kg in a single injection. The respective response rate for HCG, Ovaprim, and cGnRH IIa among females was 88.9, 77.8, and 50%. Mean latent period for HCG, Ovaprim, and cGnRH IIa among females was 33.4, 34.4, and 34.7 h. In season two, four treatments were used: saline, HCG, cGnRH IIa, and cGnRH IIa with the dopamine inhibitor domperidone (cGnRH IIa+D). Males did not express milt in either season, so they were euthanized to collect testes and prepare a fertilization solution. Spawns were segregated by spawning agent and incubated in hatching jars. Response rates of females to saline, HCG, cGnRH IIa, and cGnRH IIa+D were 0, 75, 90, and 75%, respectively. There was no statistically significant difference among the treatments for response, eggs per kg of female body weight, number of eggs/g, or latent period. Hatched larvae yield from hatching jars ranged from 0.5% to 19.5%. The spawning agent cGnRH IIa had similar efficacy as commercially available HCG. This investigation also examined the efficacy of a fertilization solution generated following extraction and maceration of testes. Testes of mean weight 6.6 g yielded spermatozoa at a mean density of 6.0 × 109 spermatozoa/mL. There was no significant effect of inducing agent on sperm density of solution. Spermatozoa were characterized in three groups regarding motility: nonmotile, progressively motile (desired), and non‐progressively motile. Sperm motility videos revealed a mean progressive motility of 7.9%. Mean values for nonmotile and non‐progressively motile spermatozoa were 75.5% and 16.6%, respectively. Mean duration of motility was 116 s.

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Section: Resultsmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Evaluation of Hormonal Agents for Artificial Propagation of Largemouth Bass

2021

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“…The fish were divided into four groups (A‒D, each group = 25), acclimatized in four separate indoor tanks (3.6 × 3.1 × 1.0 m 3 ) with suitable water temperature and dissolved oxygen, and fed commercial food twice per day. Groups fish in A, B and C were intraperitoneally injected with LHRHa2 (2.5 µg/kg body weight), DOM (2.5 mg/kg body weight), and both LHRHa2 and DOM once a week, respectively, based on previous studies (Li et al., 2020; Porter, 1997; Shi et al., 2015). The fish in group D (control group) were injected with an equal volume of physiological saline (PS, 0.9% NaCl).…”

Section: Methodsmentioning

confidence: 99%

“…LMB are semi‐synchronous reproducers, with the females typically containing oocytes in two or more developmental stages (Martyniuk et al., 2013). In recent decades, several studies have reported that LHRHa, hCG, and DOM effectively induce ovulation of cultured LMB (Li et al., 2020; Porter, 1997). However, there is no information on hormones that affect GtH subunits expression and gonadal development at the primary growth phase of this species.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of gonadotropin subunits (fshβ, lhβ and cgα) of largemouth bass ( micropterus salmoides ) and their expression in response to luteinizing hormone‐releasing hormone analogue and dopamine antagonists

Sun

et al. 2021

J Appl Ichthyol

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Gonadotropins (GtHs), including follicle‐stimulating hormone (FSH) and luteinizing hormone (LH), are important hormones involved in gametogenesis, gonadal steroidogenesis, and maturation. In this study, GtH subunits (FSHβ, LHβ and CGα) of largemouth bass (Micropterus salmoides) were cloned and characterized, and their regulation by luteinizing hormone‐releasing hormone analog (LHRHa2) and dopamine antagonist (DOM) treatment in vivo was investigated. The full‐length cDNA sequences of FSHβ, LHβ, and CGα were 683, 576, and 685bp, encoding 120, 152, and 132 amino acids, respectively. The deduced amino acid sequence analysis revealed that GtH subunits contain conserved cysteine residues and potential N‐linked glycosylation sites and showed high homology with the corresponding subunit sequences from other Perciformes by phylogenetic analysis. In the primary growth cortical alveoli stage of largemouth bass, the three GtH subunits were highly expressed in the pituitary, brain and ovary, but weakly in other tissues. After LHRHa2 and DOM injection, mRNA levels of FSHβ, LHβ, and CGα in the pituitary were significantly increased at 12 or 24 hr (p <.05), but significantly decreased at 48 hr; plasma FSH and LH levels showed a consistent trend. Histological analysis classified ovaries from LHRHa2‐ and DOM‐treated fish into the early oocyte maturation stage and late vitellogenesis stage, while those from the control group fish were classified into the early vitellogenesis stage. These results suggested that both LHRHa2 and DOM stimulated GtH subunit expression, increased plasma FSH and LH and accelerated ovary development of largemouth bass, providing a framework for a better understanding of the mechanisms of hormone‐mediated reproduction control in teleosts.

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