Oocyte Maturation A story of arrest and release

Sen, Aritro; Caiazza, Francesco

doi:10.2741/s383

Cited by 69 publications

(53 citation statements)

References 280 publications

(398 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Downregulated genes involved in gap junctions and the cell cycle Extrusion of the first polar body from fully grown oocytes is another distinctive process defined as oocyte meiotic maturation or nuclear maturation (Sen and Caiazza 2013). It is well known that high concentrations of cAMP and cGMP in the oocyte cytoplasm lead to meiotic arrest, and that decreases in cAMP and cGMP concentrations in oocytes lead to resumption of meiosis to MII (Sánchez and Smitz 2012).…”

Section: Upregulated Genes Involved In Egf and Ecmmentioning

confidence: 99%

Genomic expression profiles in cumulus cells derived from germinal vesicle and MII mouse oocytes

Chian

et al. 2016

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

Cumulus cells (CCs) are distinct from other granulosa cells and the mutual communication between CCs and oocytes is essential for the establishment of oocyte competence. In the present study we assessed genomic expression profiles in mouse CCs before and after oocyte maturation in vitro. Microarray analysis revealed significant changes in gene expression in CCs between the germinal vesicle (GV) and metaphase II (MII) stages, with 2615 upregulated and 2808 downregulated genes. Genes related to epidermal growth factor, extracellular matrix (Ptgs2, Ereg, Tnfaip6 and Efemp1), mitochondrial metabolism (Fdx1 and Aifm2), gap junctions and the cell cycle (Gja1, Gja4, Ccnd2, Ccna2 and Ccnb2) were highlighted as being differentially expressed between the two development stages. Real-time polymerase chain reaction confirmed the validity and reproducibility of the results for the selected differentially expressed genes. Similar expression patterns were identified by western blot analysis for some functional proteins, including EFEMP1, FDX1, GJA1 and CCND2, followed by immunofluorescence localisation. These genes may be potential biomarkers for oocyte developmental competence following fertilisation and will be investigated further in future studies.

show abstract

Section: Upregulated Genes Involved In Egf and Ecmmentioning

confidence: 99%

Genomic expression profiles in cumulus cells derived from germinal vesicle and MII mouse oocytes

Chian

et al. 2016

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

show abstract

“…In some clinical studies where endogenous LH was absent or inactive, recombinant human FSH alone allowed follicle development but with an inadequate estradiol concentration [22]. Although LH has essential and wellestablished roles in ovarian steroid synthesis and ovulation [23][24][25], the use of LH in superovulation treatments is controversial and unclear. Low LH levels might intensify FSH sensitivity in granulosa cells by increasing androgen synthesis during the early stage of folliculogenesis and this activity is required for normal follicle and oocyte development [26,27].…”

Section: Introductionmentioning

confidence: 99%

Effect of corifollitropin alfa supplemented with or without LH on ovarian stimulation and embryo viability in rabbit

et al. 2017

View full text Add to dashboard Cite

There is increasing interest in using rabbits for research as a laboratory model as well as for industrial production of meat, wool and fur. Superovulation in animals is used to produce a maximum number of transferable embryos per donor, in order to either support genetic improvement programs, ex situ conservation or to optimize other biotechnologies. Over time, the use of this biotechnology has shown variable outcomes as a consequence of several factors, such as the origin of exogenous hormone, posology and the effect of gonadotropins used simultaneously, the donor and the environment. The aim of this study was to compare the efficacy of a single injection of corifollitropin alfa (CTP), alone or supplemented with LH, versus a FSH standard protocol of five equal doses administered twice daily to superovulate rabbit does (20 per group and 29 control females). We determined: 1) the impact of this stimulation on in vitro development and mRNA expression at blastocyst stage and 2) in vivo embryo development and viability rate at birth of transferred embryos. Our outcomes showed that the ovulation rate was similar among the different ovarian stimulation groups, reaching more than fourfold the ovulation rate of a control doe. While rates of embryos developing to the blastocyst stage after 48 h of in vitro culture were similar between groups, the hatched blastocyst rate was higher for superovulated embryos from CTP group. Moreover, no significant differences among mRNA expression of OCT4, SOX2 and NANOG genes were detected. Nevertheless, embryos from ovarian stimulated does with CTP + LH showed significantly higher implantation rates and survival at birth among the different ovarian stimulation groups and similar to those in the control group. In conclusion, the results of this study suggest that a single injection of long acting corifollitropin alfa can be effectively used in rabbits to elicit a more than fourfold increase in ovulation rate compared to control animals. In addition, the LH supplementation allows us to obtain similar in vivo embryo development results as in the control group.

show abstract

“…LH has a number of roles in follicular development [2] and in the periovulatory phase, LH is involved in the induction of ovulation [2], completion of meiosis I [3], early luteinization and the production of progesterone [4]. Ovarian steroidogenesis can be driven by activation of a low number (around 1%) of LH receptors and, during cycles of assisted reproductive technology (ART), adequate levels of endogenous LH are usually present despite pituitary suppression with gonadotrophin-releasing hormone (GnRH) analogues [5,6].…”

Section: Introductionmentioning

confidence: 99%

Recombinant human follicle-stimulating hormone (r-hFSH) plus recombinant luteinizing hormone versus r-hFSH alone for ovarian stimulation during assisted reproductive technology: systematic review and meta-analysis

Lehert

Kolibianakis

Venetis

et al. 2014

Reprod Biol Endocrinol

107

View full text Add to dashboard Cite

BackgroundThe potential benefit of adding recombinant human luteinizing hormone (r-hLH) to recombinant human follicle-stimulating hormone (r-hFSH) during ovarian stimulation is a subject of debate, although there is evidence that it may benefit certain subpopulations, e.g. poor responders.MethodsA systematic review and a meta-analysis were performed. Three databases (MEDLINE, Embase and CENTRAL) were searched (from 1990 to 2011). Prospective, parallel-, comparative-group randomized controlled trials (RCTs) in women aged 18–45 years undergoing in vitro fertilization, intracytoplasmic sperm injection or both, treated with gonadotrophin-releasing hormone analogues and r-hFSH plus r-hLH or r-hFSH alone were included. The co-primary endpoints were number of oocytes retrieved and clinical pregnancy rate. Analyses were conducted for the overall population and for prospectively identified patient subgroups, including patients with poor ovarian response (POR).ResultsIn total, 40 RCTs (6443 patients) were included in the analysis. Data on the number of oocytes retrieved were reported in 41 studies and imputed in two studies. Therefore, data were available from 43 studies (r-hFSH plus r-hLH, n = 3113; r-hFSH, n = 3228) in the intention-to-treat (ITT) population (all randomly allocated patients, including imputed data). Overall, no significant difference in the number of oocytes retrieved was found between the r-hFSH plus r-hLH and r-hFSH groups (weighted mean difference −0.03; 95% confidence interval [CI] −0.41 to 0.34). However, in poor responders, significantly more oocytes were retrieved with r-hFSH plus r-hLH versus r-hFSH alone (n = 1077; weighted mean difference +0.75 oocytes; 95% CI 0.14–1.36). Significantly higher clinical pregnancy rates were observed with r-hFSH plus r-hLH versus r-hFSH alone in the overall population analysed in this review (risk ratio [RR] 1.09; 95% CI 1.01–1.18) and in poor responders (n = 1179; RR 1.30; 95% CI 1.01–1.67; ITT population); the observed difference was more pronounced in poor responders.ConclusionsThese data suggest that there is a relative increase in the clinical pregnancy rates of 9% in the overall population and 30% in poor responders. In conclusion, this meta-analysis suggests that the addition of r-hLH to r-hFSH may be beneficial for women with POR.

show abstract

Oocyte Maturation A story of arrest and release

Cited by 69 publications

References 280 publications

Genomic expression profiles in cumulus cells derived from germinal vesicle and MII mouse oocytes

Genomic expression profiles in cumulus cells derived from germinal vesicle and MII mouse oocytes

Effect of corifollitropin alfa supplemented with or without LH on ovarian stimulation and embryo viability in rabbit

Recombinant human follicle-stimulating hormone (r-hFSH) plus recombinant luteinizing hormone versus r-hFSH alone for ovarian stimulation during assisted reproductive technology: systematic review and meta-analysis

Contact Info

Product

Resources

About