Oocyte-expressed genes affecting ovulation rate

McNatty, Kenneth P.; Smith, Peter; Moore, L. G.; Reader, Karen L; Lun, S.; Hanrahan, J. P.; Groome, Nigel P.; Laitinen, Mika; Ritvos, Olli; Juengel, Jennifer L.

doi:10.1016/j.mce.2004.08.013

Cited by 129 publications

(112 citation statements)

References 44 publications

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“…Significantly, those with heterozygous mutations in both GDF9 and BMP15 had ovulation rates that were greater than additive for each mutation alone. 42 Depending on the location of the eight known mutations in BMP15, the increase in ovulation rate in heterozygous ewes was between 16 and 100% above that of their respective wild-type counter parts. 42,43 For GDF9, the data from the heterozygous Belclare/Cambridge mutant indicates an ovulation rate increase of 87% above that of the wild-type.…”

Section: 4mentioning

confidence: 99%

Oocyte-Somatic Cell Interactions and Ovulation Rate: Effects on Oocyte Quality and Embryo Yield

McNatty¹,

Juengel²,

Pitman³

2014

Reproductive Biology Insights

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ABSTRACT:The use of exogenous gonadotropins to increase oocyte yields for better pregnancy outcomes remains unpredictable and inefficient. The objectives of this review are to address two questions concerning this issue: (1) are there any alternatives for improving consistency in oocyte yields? (2) is it possible to develop a molecular diagnostic test for oocytes with blastocyst potential? Studies in sheep with a heterozygous inactivating mutation in the oocyte-derived growth factor, bone morphogenetic protein 15 (BMP15), report increased oocyte yields and significantly more offspring. Moreover, partial immuno-neutralization of BMP15 bioactivity increases oocyte yield without modifying gonadotropin or ovarian steroid secretion. Therefore, it is hypothesized that the development of BMP15 antagonists may prove them to be suitable alternatives to exogenous gonadotropins. Recent evidence from analyses of candidate gene expression in human cumulus cells separated from individual oocytes before IVF indicates a potential non-invasive molecular screen for oocytes with improved blastocyst and live-birth outcomes.

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Section: 4mentioning

confidence: 99%

Oocyte-Somatic Cell Interactions and Ovulation Rate: Effects on Oocyte Quality and Embryo Yield

McNatty¹,

Juengel²,

Pitman³

2014

Reproductive Biology Insights

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“…BMP15 and its closest homolog growth differentiation factor-9 (GDF9) (McPherron and Lee 1993;McGrath et al 1995;Dube et al 1998;Hayashi et al 1999;Jaatinen et al 1999) play crucial roles in the regulation of fertility (Gilchrist et al 2004a;McNatty et al 2005c). Mutations in these genes lead to reproductive defects in animals and with the recombinant forms of these growth factors the actions of the oocyte can be mimicked (Gilchrist et al 2004b).…”

Section: Introductionmentioning

confidence: 99%

The bioactivity of human bone morphogenetic protein-15 is sensitive to C-terminal modification: Characterization of the purified untagged processed mature region

Pulkki

Myllymaa

Pasternack

et al. 2011

Molecular and Cellular Endocrinology

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“…the number of mature oocytes released during one reproductive cycle) in mammals is determined by a complex exchange of hormone signals between the pituitary gland and the ovary, and by a localized exchange of hormones within ovarian follicles between the oocyte and its adjacent somatic cells (Galloway et al, 2000;Eppig, 2001). Many mammals including primates, goats, cattle, deer and possums normally have an ovulation rate of one or sometimes two whereas other mammals such as rats, mice, hamsters, cats, dogs and pigs have ovulation rates that vary between four and 15 (McNatty et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Lack of polymorphism in the oocyte derived growth factor (GDF9) gene in the Shal breed of sheep

2010

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The families of TGF-β proteins are the most important growth factors in the ovary for growth and differentiation of early ovarian follicles. Three related oocyte-derived members of the transforming growth factor-β superfamily, namely GDF9, BMP15 and BMPR-IB have been shown to be essential for follicular growth and ovulation. The aim of the present study was to detect the incidence of mutation in exon two of GDF9 as a major gene in the Shal sheep breed. Blood samples were collected from 239 sheep and genomic DNA was extracted using the modified salting-out method. The quantity and quality of extracted DNA was examined using spectrophotometery and gel electrophoresis, respectively. A fragment with the size of 139 bp from exon two of GDF9 gene (FecG H ) was amplified using polymerase chain reaction (PCR) with a pair of specific primers. The amplified PCR products were digested with DdeI restriction enzyme. In the presence of mutations at this locus, the DdeI enzyme cannot recognize the restriction site. However, in the absence of mutations, the enzyme recognizes one restriction site and divides the amplified fragment into two fragments of 31 and 108 bp. In the present study only the wild type alleles were detected and all the samples showed the AA genotype. The analysis of polymorphism for GDF9 (FecG H ) loci in Shal sheep indicates that the genetic factor responsible for twinning or multiple lambing rates is not related to reported mutated alleles at the GDF9 major gene in this breed. Therefore, we should attempt to detect other SNP for the GDF9 gene and/or other loci responsible for twining rate in this breed. ________________________________________________________________________________

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Oocyte-expressed genes affecting ovulation rate

Cited by 129 publications

References 44 publications

Oocyte-Somatic Cell Interactions and Ovulation Rate: Effects on Oocyte Quality and Embryo Yield

Oocyte-Somatic Cell Interactions and Ovulation Rate: Effects on Oocyte Quality and Embryo Yield

The bioactivity of human bone morphogenetic protein-15 is sensitive to C-terminal modification: Characterization of the purified untagged processed mature region

Lack of polymorphism in the oocyte derived growth factor (GDF9) gene in the Shal breed of sheep

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