Oocyte dysmorphism and aneuploidy in meiotically mature human oocytes after ovarian stimulation

Blerkom, Jonathan Van; Henry, George P.

doi:10.1093/oxfordjournals.humrep.a137655

Cited by 177 publications

(90 citation statements)

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“…The resulting oxidative stress can contribute to a pro-inflammatory status that may enhance insulin resistance and hyperandrogenism in women with PCOS [48][49][50].…”

Section: Discussionmentioning

confidence: 99%

Protein modification as oxidative stress marker in follicular fluid from women with polycystic ovary syndrome: the effect of inositol and metformin

Piomboni

Focarelli

Capaldo

et al. 2014

J Assist Reprod Genet

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Purpose The purpose of this study was to evaluate the oxidative stress status (OS) of follicular fluid (FF) and the oocyte quality in women with polycystic ovary syndrome (PCOS) undergoing different ovarian stimulation protocols. Methods FF samples were collected after gonadotropin administration in association or not with metformin or D-chiro-inositol (DCI). OS status was then evaluated by checking the follicular fluid protein oxidation profile after specific labeling of aminoacidic free-SH groups, and two-dimensional electrophoresis followed by qualitative and semiquantitative analysis. Oocyte quality was assessed by international morphological criteria. Results Our data indicated that both treatments, even if to different extent, recovered a significantly high level of free-SH groups in FF proteins of PCOS women clearly indicating a decrease of OS level with respect to that found in FF samples from gonadotropins alone treated women. A higher number of good quality MII oocytes was also observed in DCI (P<0.05) or metformin (P<0.05) study groups in comparison to untreated control group. Conclusion A natural supplement and a drug both showed a statistically significant positive effect on follicular milieu by decreasing the oxidative damage on FF proteins, as well as in recovering good quality oocytes.

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“…The resulting oxidative stress can contribute to a pro-inflammatory status that may enhance insulin resistance and hyperandrogenism in women with PCOS [48][49][50].…”

Section: Discussionmentioning

confidence: 99%

Protein modification as oxidative stress marker in follicular fluid from women with polycystic ovary syndrome: the effect of inositol and metformin

Piomboni

Focarelli

Capaldo

et al. 2014

J Assist Reprod Genet

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“…1a). The presence of vacuoles or inclusions within the cytoplasm was also considered because the presence of these dysmorphisms is considered an abnormality [42][43][44]49]. Basically, oocytes lacking both vacuoles and inclusions were awarded three points, oocytes with either small amounts of vacuoles or inclusions two points, and oocytes containing both vacuoles and inclusions one point (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…For example, regular patterns of granularity have been observed in the oocyte cytoplasm and it has been suggested that these patterns correlate with oocyte quality [44,45]. Human oocytes may contain inclusions or vacuoles which may be detrimental to the implantation potential of this material [37,38,[41][42][43].…”

Section: Introductionmentioning

confidence: 99%

An oocyte score for use in assisted reproduction

Wilding¹,

Matteo

D’Andretti³

et al. 2007

J Assist Reprod Genet

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Purpose In this work, we describe a system for the morphological scoring of human oocytes prior to fertilisation and use this system to test whether oocyte morphology is an indicator of fertilisation, embryo development and implantation potential. Methods The study is a prospective trial of the use of oocyte morphological scores in 822 patients undergoing their first cycle of ICSI. Analyses of oocytes were performed prior to ICSI procedures and the scores compared with fertilisation rates, embryo quality and clinical results.Results 'Top quality' oocytes had a significantly higher level of fertilisation (96%) as compared to low scoring oocytes (25.6%). Where top quality oocytes formed top quality embryos, we noted a clinical success rate of 63.4%. Conclusions Clinical success rates were increased in cases where top quality oocytes formed top quality embryos after ICSI. The analysis of oocyte morphology may represent a positive selection feature during ICSI.

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“…10 The Center for Disease Control reported that ICSI was used in up to 78% of non-male factor ART cycles in the USA. 11 In fact, ICSI overcomes some IVF difficulties, such as zona pellucida abnormalities that prevents sperm fusion to the oolema; 12,13 zona pellucida hardening and consequent inhibition of natural sperm penetration in cryopreserved oocytes; 14,15 and DNA contamination from additional sperm that would be adhering to the zona pellucida in preimplantation genetic diagnosis (PGD) cycles. 16 Overall, the use of ICSI has not been shown to cause any more negative effects than those seen with IVF.…”

Section: Introductionmentioning

confidence: 99%

Overcoming male factor infertility with intracytoplasmic sperm injection

Borges

Zanetti

Braga

et al. 2017

Rev. Assoc. Med. Bras.

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Objective:To evaluate the effect of male factor infertility on intracytoplasmic sperm injection (ICSI) outcomes compared with a control group presenting isolated tubal factor. Method: This retrospective study included 743 couples undergoing ICSI as a result of isolated male factor and a control group consisting of 179 couples undergoing ICSI as a result of isolated tubal factor, performed in a private university--affiliated in vitro fertilization center, between January/2010 and December/2016. Patients were divided into two groups according to maternal age: women ≤35 years old and >35 years old. The effects of infertility causes on laboratorial and clinical ICSI outcomes were evaluated using Student's t-test and χ 2 test. Results: No differences in controlled ovarian stimulation outcomes were observed between male factor cycles and tubal factor cycles in the two age groups. Implantation (male factor 35.5% vs. tubal factor 32.0%, p=0.340), pregnancy (male factor 46.9% vs. tubal factor 40.9%, p=0.184) and miscarriage (male factor 10.3% vs. tubal factor 10.6%, p=0.572) rates were similar between the infertility groups, irrespective of female age. Considering maternal age, the cancelation rate was higher in older women (>35 years old) undergoing ICSI as a result of male factor infertility (17.4% vs. 8.9%, p=0.013). Conclusion: Our results showed that there is no difference in the outcomes of pregnancy between couples with male or tubal factor infertility, which indicates that ICSI surpasses the worse specific outcomes associated with male factor.

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Oocyte dysmorphism and aneuploidy in meiotically mature human oocytes after ovarian stimulation

Cited by 177 publications

References 0 publications

Protein modification as oxidative stress marker in follicular fluid from women with polycystic ovary syndrome: the effect of inositol and metformin

Protein modification as oxidative stress marker in follicular fluid from women with polycystic ovary syndrome: the effect of inositol and metformin

An oocyte score for use in assisted reproduction

Overcoming male factor infertility with intracytoplasmic sperm injection

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