Oocyte donation using cryopreserved donor oocytes

Yang, Dunsong; Winslow, Kevin L.; Blohm, Patrick; Brown, Samuel; Nguyen, Kevin A.; Brubaker, Cindy

doi:10.1016/s0015-0282(02)03417-9

Cited by 54 publications

(25 citation statements)

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“…Die erste Schwangerschaft nach der Befruchtung kryokonservierter Oozyten wurde in den 1980er-Jahren publiziert [19]. Allerdings führte erst die Verbesserung der Labortechniken, insbesondere die Einführung von hochkonzentrierter 7 Sucroselösung als Kryoprotektivum zu Schwangerschaftsraten, die den Einsatz dieses Verfahrens bei speziellen Indikationen zunehmend ermöglichen, sodass mittlerweile Ergebnisse erreicht werden, die den Zahlen der Kryokonservierung fertilisierter Eizellen nahekommen [20,21]. Die Ergebnisse lassen sich aus den Zahlen großer italienischer Zentren ablesen, die diese Technik aufgrund der strikten italienischen Gesetzgebung standardmäßig in der Kinderwunschbehandlung einsetzen.…”

Section: Kryokonservierung Von Unbefruchteten Oozytenunclassified

Fertilitätserhalt bei Krebs

2009

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Section: Kryokonservierung Von Unbefruchteten Oozytenunclassified

Fertilitätserhalt bei Krebs

2009

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“…It would be a useful treatment option for women who are at risk of losing their ovarian function because of pelvic diseases, surgery or radio/ chemotherapy and could also avoid some of the moral and ethical problems posed by embryo freezing. Although several pregnancies and live births following freezing and thawing of human oocytes have been reported [1][2][3][4][5][6][7][8][9][10][11][12][13], human oocyte freezing as a technology is still considered to be at an early developmental stage. Improving pregnancy rates associated with the use of cryopreserved human oocytes would be a major advance in human ART.…”

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confidence: 99%

“…Although live young have been produced from cryopreserved oocytes in the mouse using a slow Accepted for publication: September 22, 2004 Correspondence: R-C.Chian (e-mail:ri-cheng.chian@muhc.mcgill.ca) freezing [14], the method has met very limited success in other species [15,16] including humans [1][2][3][4][5][6][7][8][9]. Mammalian oocytes have proven to be more difficult to cryopreserve than cleavage-stage embryos [17], and the efficiency of oocyte cryopreservation in most species, except for the mouse, is still very low (for a review, see [18]).…”

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confidence: 99%

High Survival Rate of Bovine Oocytes Matured In Vitro Following Vitrification

Chian

Kuwayama

Tan

et al. 2004

Journal of Reproduction and Development

162

100

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Abstract. Improving pregnancy rates associated with the use of cryopreserved human oocytes would be an important advance in human assisted reproductive technology (ART). Vitrification allows glasslike solidification of a solution without ice crystal formation in the living cells. We have attempted to improve the survival rates of oocytes by a vitrification technique using bovine models. In vitro matured oocytes with or without cumulus cells were vitrified with either 15.0% (v/v) ethylene glycol (EG) + 15% (v/v) dimethylsulfoxide (DMSO) + 0.5 M sucrose or 15% (v/v) EG + 15% (v/v) 1,2-propanediol (PROH) + 0.5 M sucrose, using 'Cryotop' or 'thin plastic sticker', respectively. The oocyte survival rates after vitrifying-warming, and the capacity for fertilization and embryonic development were examined in vitro. The rate of embryonic development to blastocyst was significantly higher (P<0.05) in the oocytes vitrified with 15% (v/v) EG + 15% (v/v) PROH + 0.5 M sucrose than in the oocytes vitrified with 15% (v/v) EG + 15% (v/v) DMSO + 0.5 M sucrose (7.4% ± 4.1 vs. 1.7% ± 3.0, respectively). Oocytes vitrified without cumulus cells had a higher survival rate after thawing and a superior embryonic developmental capacity compared with oocytes vitrified with cumulus cells. Prolonged pre-incubation time after thawing adversely affected the rates of embryonic cleavage and development. These results indicate that in vitro matured bovine oocytes can be vitrified successfully with the mixture of the cryoprotectants, EG + PROH, the absence of cumulus cells for vitrification does not affect oocyte survival rate after warming, and vitrified and warmed oocytes do not require pre-incubation before in vitro fertilization.

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“…To date, more than 1,000 successful live births resulting from cryo-preserved human oocytes have been reported [2][3][4][5][6][7][8][9][10][11][12]. Vitrification seems to be a successful and promising technique for oocyte cryopreservation, resulting in several hundred healthy live births recently [10,11,[13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Effects of duration of cryo-storage of mouse oocytes on cryo-survival, fertilization and embryonic development following vitrification

Yan

Suzuki

et al. 2011

J Assist Reprod Genet

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Purpose To investigate the effects of cryo-storage duration in liquid nitrogen on oocyte cryo-survival, fertilization and embryonic development following vitrification and warming. Methods Mature mouse oocytes were vitrified with McGill Cryoleaf and stored in liquid nitrogen for a period of 8-10 days, 90-92 days and 180-182 days, respectively. After warming, the survived oocytes were inseminated by intracytoplasmic sperm injection (ICSI) and cultured for 120 h. The rates of oocyte cryo-survival, cleavage and embryonic development were compared. Result(s)The oocyte cryo-survival rate declined following cryo-storage duration for 180-182 days (90.4±7.9%) compared to that of the other two groups (97.4±3.0% and 98.0± 3.3%). The fertilization rate in the group of 180-182 days (66.6±22.0%) was also significantly reduced (P<0.05) compared with the groups of 8-10 days (92.2±10.8%) and 90-92 days (94.7±9.1%). In addition, the number of embryos developed to the blastocyst stage declined significantly (P<0.05) following long cryo-storage duration (72.1± 8.2%, 25.2±3.8% and 5.5±13.6%, respectively). Conclusion(s) The cryo-survival, fertilization rate and embryonic development of mouse oocytes are affected significantly, in an adverse manner, by the cryo-storage duration in liquid nitrogen.

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Oocyte donation using cryopreserved donor oocytes

Cited by 54 publications

References 0 publications

Fertilitätserhalt bei Krebs

Fertilitätserhalt bei Krebs

High Survival Rate of Bovine Oocytes Matured In Vitro Following Vitrification

Effects of duration of cryo-storage of mouse oocytes on cryo-survival, fertilization and embryonic development following vitrification

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