Mahseer, Tor tambroides locally known as kelah and empurau, is known as one of the highest valued freshwater fish in Malaysia (Asaduzzaman et al., 2016), where the price was recorded between USD180 and USD280 kg −1 (UPM, 2013) in recent years. However, the aquaculture of T. tambroides is yet to be developed due to difficulties in obtaining and maintaining good quality mature broodstock.Often in captivity, reproductive performance of female broodstock depreciates over time resulting in the production of an inconsistent number of eggs and viable larvae (Abrehouch et al., 2007;Papanikos et al., 2003).Hormone and nutrition are two interrelated factors that determine the success of breeding performance in aquaculture species (Ingram et al., 2007;Izquierdo et al., 2001). Hormonal injections are widely used in induced breeding of fish broodstock in captivity for cyprinid fish (Podhorec et al., 2016;Xu et al., 2017). It was also used in the breeding induction of both Malaysian mahseer,