Oocyte Denuding

Rienzi, Laura; Maggiulli, Roberta; Ubaldi, Filippo Maria

doi:10.1007/978-3-319-43011-9_14

Cited by 4 publications

(3 citation statements)

References 136 publications

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“…Next, the oocyte was identified in the horizontal laminar flow hood with the Olympus stereo microscope, and the oocyte was clearly visible. Then, oocytes were denudated from cumulus oophorus by exposure to an 80 IU/mL hyaluronidase enzyme (12) in the HEPESbuffered medium, followed by the mechanical removal of the corona radiata. In addition, oocytes were assessed for their maturation and morphology under an inverted microscope.…”

Section: Oocyte Retrievalmentioning

confidence: 99%

Comparison of Oocyte and Embryo Quality in Women With Polycystic Ovary Syndrome and the Control Group Candidate for In Vitro Fertilization and Intracytoplasmic Sperm Injection

Afiat

Khadem

Nayeri

et al. 2020

International Journal of Women’s Health and Reproduction Scienc

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Objectives: Polycystic ovary syndrome (PCOS) is the most common cause of female infertility. The aim of this study was to compare the oocyte and embryo quality between the PCOS women with the control group candidate for in vitro fertilization/ intracytoplasmic sperm injection (IVF/ICSI). Materials and Methods: The present study was designed at the Infertility Research Center of Milad in the prospective cohort format and was carried out on 100 cases of infertile women with confirmed PCOS (case group) and the male factor (control group) as the first IVF cycle candidates. Both groups underwent the ovary stimulation cycle and ICSI under the standard antagonist protocol. The collected data were then processed and analyzed using the SPSS software, version 16. Results: The average age of study cases was 35±3, and oocyte necrosis was the underlying pathological factor in both groups (28% and 26% in the PCOS and control groups, respectively). In addition, most embryones belonged to either grade 1 or 2 or were 8-cell embryos. Furthermore, the highest number of transferred embryos among the patients was related to the 8-cell and grade 1. The occurrences of biochemical pregnancy in the PCOS and control groups were up to 31.91% and 22%, respectively, leading to 72.73% and 60% childbirth in cases of both groups. Finally, there were no significant differences observed with respect to the quality and the quantity of the embryones, the oocyte, the transferred embryo, the germinal vesicle oocytes, and the rate of pregnancy among the two groups (P>0.05). Conclusions: According to the results of the present study, no differences were found concerning the oocyte quality, embryo, and the pregnancy rate between PCOS cases and any other patients requiring ICSI. Therefore, such cases can similarly benefit from ICSI methods as well.

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Section: Oocyte Retrievalmentioning

confidence: 99%

Comparison of Oocyte and Embryo Quality in Women With Polycystic Ovary Syndrome and the Control Group Candidate for In Vitro Fertilization and Intracytoplasmic Sperm Injection

Afiat

Khadem

Nayeri

et al. 2020

International Journal of Women’s Health and Reproduction Scienc

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“…Concerning the ultra-rapid freezing systems, pellets and straws were chosen for their ease of application. Other devices usually employed for oocyte vitrification [Cryotops, ( 54 )] or denudation [stripper tips, ( 55 )] have been tested for spermatozoa vitrification ( 16 , 56 ), as well as carrier systems specifically designed for this purpose, such as the SpermVD ( 57 ) or others ( 58 ). In this study, pellets and straws were basically comparable, likely because the volume of ultra-rapid frozen spermatozoa was the same in both the systems and the presence of the straw did not have a significant influence on cooling rates.…”

Section: Discussionmentioning

confidence: 99%

Ultra-Rapid Freezing Preserves Morphofunctional Integrity and Fertilizing Ability of Epididymal Cat Spermatozoa

et al. 2022

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Vitrification and ultra-rapid freezing, which are more commonly used for oocytes and embryos, have recently been applied to spermatozoa in an attempt to make semen cryopreservation in field conditions easier compared to conventional freezing. It is well-known that in case of unexpected death of rare and wild animals, preserving epididymal spermatozoa from isolated testicles represents a great chance of salvaging male germplasm for future use in assisted reproductive technologies. The aim of this study was to evaluate the morphofunctional integrity of cat epididymal spermatozoa ultra-rapid frozen in pellets or straws with two different extenders [E1 (Tris buffer with 20% egg yolk and 0.25 M sucrose) or E2 (Ham's F10 with 1% bovine serum albumin and 0.4 M sucrose)] and to test whether spermatozoa preserved by the best combination were able to fertilize oocytes and produce embryos in vitro by intracytoplasmic sperm injection (ICSI) of in vitro matured cat oocytes. The results showed that E1 and E2 in straw or pellet were comparable (at warming, about 30% normal morphology, 45% intact membranes, and 20% intact acrosomes), except for post-warming motility that was better maintained along time by E1 pellet (21.7 ± 7.4% at warming and 3.6 ± 2.9% after 6 h). Such spermatozoa could fertilize conspecific oocytes and support embryonic development (cleavage 35.5%) as well as frozen control spermatozoa (cleavage 54.29%, p = 0.22). In conclusion, cat epididymal spermatozoa better maintained their morphofunctional features after ultra-rapid freezing with E1 and could successfully produce embryos in vitro after ICSI. This underscores their usefulness as cryobanked material for fertility and biodiversity preservation purposes.

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“…Oocyte denudation is a crucial prerequisite for unequivocal evaluation of oocyte maturity and successful intracytoplasmic sperm injection. 1,2 Residual cumulus cells can potentially prevent the oocyte from being adequately manipulated and may represent a source of DNA contamination during trophectoderm biopsy for PCR-based preimplantation genetic testings. 3,4 The established manual denuding method, developed decades ago, consists of two steps: the enzymatic digestion and then the mechanical stripping of the cumulus using pipettes.…”

Section: Introductionmentioning

confidence: 99%