Oocyte Bone Morphogenetic Protein 15, but not Growth Differentiation Factor 9, Is Increased During Gonadotropin-Induced Follicular Development in the Immature Mouse and Is Associated with Cumulus Oophorus Expansion1

Guéripel, Xavier; Brun, Véronique; Gougeon, Alain

doi:10.1095/biolreprod.106.055574

Cited by 74 publications

(56 citation statements)

References 41 publications

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“…FF BMP-15 level of fertilized oocytes were significantly higher than that of unfertilized oocytes [6]. Gueripel et al,observed increased BMP-15 immunostaining on ovarian tissue of mouse stimulated with gonadotropins [15]. In our population; D3 BMP-15 level showed positive correlation with progesterone level on the day of hCG injection.…”

Section: Discussionsupporting

confidence: 43%

What is the impact of serum and follicular fluid BMP-15 and AMH levels in ICSI-ET cycle outcomes?

Ilhan¹,

Özkan²,

Ekinci³

et al. 2017

Histol Cytol Embryol

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Objective: Bone morphogenetic protein-15 (BMP-15) and anti-Müllerian hormone (AMH) are the proteins functioning during ovarian follicular growth. We aimed to investigate the impact of serum and follicular fluid (FF) AMH and BMP-15 levels on outcomes of intracytoplasmic sperm injection-embryo transfer (ICSI-ET) cycles.Methods: This prospective, cross-sectional study was carried out with 82 patients attending ICSI-ET cycle. The women divided into two groups according to using either GnRH agonist (group1, N=38) or antagonist (group2, N=44) for pituitary down-regulation. Eight milliliter of serum samples were taken from the patients on the 3rd day of menstrual period (D3) and on the day of oocyte pick-up (OPU); and 8 mL of follicular fluid (FF) sample was collected during OPU. AMH and BMP-15 measurements were carried with ELISA method quantitatively. Results:The mean age and body mass index of population were 30.6 ± 5.2 years and 25.5±4.2 kg/m 2 respectively. The comparison of pregnancy rates between women according to having FF AMH (35% vs 42%) and FF BMP-15 (37.8% vs 40.5%) levels under and above of the mean values revealed no significant difference. Serum AMH levels showed negative correlation with fertilization rate (FR). D3 BMP-15 level showed positive correlation with progesterone level and endometrial thickness on the day of hCG injection.Conclusions: AMH and BMP-15 monitoring during ICSI-ET cycles for predicting cycle outcomes should be evaluated with further studies.

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Section: Discussionsupporting

confidence: 43%

What is the impact of serum and follicular fluid BMP-15 and AMH levels in ICSI-ET cycle outcomes?

Ilhan¹,

Özkan²,

Ekinci³

et al. 2017

Histol Cytol Embryol

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“…total RNA from immature mouse ovaries, Guéripel et al (2006) have observed that Bmp15 but not Gdf9 mRNA levels increased in the gonadotropin-treated immature mice. This was an in vivo study and RNA was extracted from whole ovaries.…”

Section: Gdf9 Bmp15 and Amh In Mouse Folliclesmentioning

confidence: 87%

Expression of growth differentiation factor 9, bone morphogenetic protein 15, and anti-Müllerian hormone in cultured mouse primary follicles

Sadeu

Adriaenssens²,

Smitz³

2008

Reproduction

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Growth differentiation factor 9 (GDF9), bone morphogenetic protein 15 (BMP15), and anti-Mü llerian hormone (AMH) play an important role in the primary to secondary follicle transition and follicle activation in vivo. In organ culture of neonatal mouse ovaries, it was observed that significantly fewer primary follicles develop to the secondary stage. The objectives of this study were: (1) to compare ovarian follicular populations between organ-cultured neonatal mouse ovaries and freshly isolated age-matched control ovaries; (2) to quantify RNA levels of Gdf9, Bmp15, and Amh in cultured primary follicles; and (3) to immunolocalize GDF9 and AMH in cultured ovaries. Ovaries from 3-day-old (PND 3) mice were cultured for 7 or 10 days in the absence or presence of FSH. Follicular populations were counted in freshly isolated 13-day-old (PND 13) ovaries and organ-cultured ovaries. Transcripts were quantified in isolated primary follicles using real-time RT-PCR, and protein expressions were localized using immunohistochemistry. The number of secondary follicles in organ-cultured ovaries was significantly lower than in vivo controls. Gdf9 and Bmp15 mRNA expression levels were similar as in controls. Amh mRNA levels were significantly (P!0.05) lower after day 10 of culture in the absence of FSH. GDF9 and AMH proteins were respectively detected in the oocytes and the granulosa cells (GC) beginning at the primary and primordial stages onward. GDF9 and BMP15 production in cultured primary follicles are not different from in vivo controls; hence abnormal early follicular growth was not related to a deficient transcription of these factors.

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“…However, these authors used an immunocytochemistry method and evaluated BMP15 and GDF9 expression at the protein level in immature-stage oocytes, which differs the approach we used. Another study in an animal model suggests that oocyte treatment with rFSH increased mRNA levels of oocytesecreted factors during follicular development in parallel with oocyte maturation [35]. Thus, it is possible that overexpression of these paracrine oocyte-derived factors in PCOS oocytes relate to ovarian hyperstimulation syndrome susceptibility for patients with PCOS and exogenous gonadotropin treatment [36].…”

Section: Discussionmentioning

confidence: 99%

Single-cell expression analysis of BMP15 and GDF9 in mature oocytes and BMPR2 in cumulus cells of women with polycystic ovary syndrome undergoing controlled ovarian hyperstimulation

Resende

Vireque

Santana

et al. 2012

J Assist Reprod Genet

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Purpose To detect expression of bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) in oocytes, and their receptor type 2 receptor for BMPs (BMPR2) in cumulus cells in women with polycystic ovary syndrome (PCOS) undergoing in vitro fertilization (IVF), and determine if BMPR2, BMP15, and GDF9 expression correlate with hyperandrogenism in FF of PCOS patients. Methods Prospective case-control study. Eighteen MIIoocytes and their respective cumulus cells were obtained from 18 patients with PCOS, and 48 MII-oocytes and cumulus cells (CCs) from 35 controls, both subjected to controlled ovarian hyperstimulation (COH), and follicular fluid (FF) was collected from small (10-14 mm) and large (>18 mm) follicles. RNeasy Micro Kit (Qiagen ® ) was used for RNA extraction and gene expression was quantified in each oocyte individually and in microdissected cumulus cells from cumulus-oocyte complexes retrieved from preovulatory follicles using qRT-PCR. Chemiluminescence and RIA assays were used for hormone assays. Results BMP15 and GDF9 expression per oocyte was higher among women with PCOS than the control group. A positive correlation was found between BMPR2 transcripts and hyperandrogenism in FF of PCOS patients. Progesterone values in FF were lower in the PCOS group. Conclusion We inferred that BMP15 and GDF9 transcript levels increase in mature PCOS oocytes after COH, and might inhibit the progesterone secretion by follicular cells in PCOS follicles, preventing premature luteinization in cumulus cells. BMPR2 expression in PCOS cumulus cells might be regulated by androgens.

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Oocyte Bone Morphogenetic Protein 15, but not Growth Differentiation Factor 9, Is Increased During Gonadotropin-Induced Follicular Development in the Immature Mouse and Is Associated with Cumulus Oophorus Expansion1

Cited by 74 publications

References 41 publications

What is the impact of serum and follicular fluid BMP-15 and AMH levels in ICSI-ET cycle outcomes?

What is the impact of serum and follicular fluid BMP-15 and AMH levels in ICSI-ET cycle outcomes?

Expression of growth differentiation factor 9, bone morphogenetic protein 15, and anti-Müllerian hormone in cultured mouse primary follicles

Single-cell expression analysis of BMP15 and GDF9 in mature oocytes and BMPR2 in cumulus cells of women with polycystic ovary syndrome undergoing controlled ovarian hyperstimulation

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