Oocyte and embryo quality in Crassostrea gigas (Portuguese strain) during a spawning period in Algarve, South Portugal

Massapina, C.; Joaquim, Sandra; Matias, Domitília; Devauchelle, Nicole

doi:10.1016/s0990-7440(99)00115-1

Cited by 53 publications

(42 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The relationship between the reproductive cycle and energy storage and utilization cycles has already been reported by several authors for a wide variety of bivalves (e.g. Barber and Blake, 1981;Fernandez Castro and Vido de Mattio, 1987;Massapina et al, 1999;Pérez-Camacho et al, 2003). In general, reserves accumulate prior to gametogenesis in the form of glycogen, lipid and protein substrates, and are used in the production of gametes when metabolic demand is high (Mathieu and Lubet, 1993).…”

Section: Introductionmentioning

confidence: 73%

“…Intensive hatchery production of juveniles will therefore be necessary. However, inherent physiological variability among bivalve larvae can always be expected in production situations (Massapina et al, 1999), and understanding the source of this variability is crucial for reducing it. Broodstock condition is an important factor that contributes to this variability (Magnesen and Christophersen, 2008).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Reproductive activity and biochemical composition of the pullet carpet shell <i>Venerupis senegalensis</i> (Gmelin, 1791) (Mollusca: Bivalvia) from Ria de Aveiro (northwestern coast of Portugal)

Joaquim¹,

Matias²,

Matias³

et al. 2010

Sci. Mar.

View full text Add to dashboard Cite

SUMMARY: The present study characterizes the reproductive cycle of Venerupis senegalensis (=V. pullastra) from Ria de Aveiro (Portugal) as well as its nutrient storage and exploitation strategy. The reproductive cycle followed a seasonal cycle that correlated negatively with sea surface temperature, and comprised a ripe stage in winter followed by a spawning period that began in late winter and ended in the early summer. This extended spawning may be an advantageous strategy for the species because it ensures a continuous supply of settlers. Gametogenesis began in late summer/early autumn and intensified with the decrease in temperature during autumn. The condition index increased even during the spawning period, which indicates that there is rapid recovery and that reserves are accumulated during late summer and used later in the gametogenic process. Proteins did not contribute significantly to gametogenesis and the glycogen pattern is typical of conservative species, since gametogenesis depends largely on the amount of glycogen stored. The lipid storage and utilization cycle showed that gametogenesis took place in autumn/winter and that energy reserves were accumulated in summer.Keywords: pullet carpet shell, Venerupis senegalensis, biochemical composition, condition index, reproductive cycle.RESUMEN: Actividad reproductiva y composición bioquímica de la almeja babosa Venerupis senegalensis (Gmelin, 1791) (Mollusca: Bivalvia) de la Ría de Aveiro (costa noroeste de Portugal). -El presente estudio caracteriza el ciclo reproductivo de Venerupis senegalensis (= V. pullastra) en la Ría de Aveiro (Portugal) así como su estrategia de almacenamiento y explotación de los nutrientes. El ciclo reproductivo de esta especie sigue un ciclo estacional que se correlaciona negativamente con la temperatura del agua del mar, que coincide con un estado de madurez en el invierno, seguido de un periodo de desove que se inicia a finales de invierno y termina a principios del verano. Este periodo de puesta prolongado puede ser una estrategia ventajosa para la especie, ya que garantiza un suministro continuo de larvas. La gametogénesis comenzó a finales del verano/inicios de otoño y se intensificó con la disminución de la temperatura durante el otoño. El aumento del índice de condición, todavía durante la puesta, indica una recuperación rápida y una acumulación de reservas a finales del verano, que serán utilizadas en el siguiente proceso de gametogénesis. Las proteínas no contribuyen significativamente a la gametogénesis y el ciclo de glucógeno es típico de las especies conservadoras, una vez que la gametogénesis depende, en gran medida, de la cantidad de glucógeno almacenado. El almacenamiento de lípidos y su utilización indica la gametogénesis en el otoño/invierno, así como el proceso de acumulación de energía en verano.Palabras clave: almeja babosa, Venerupis senegalensis, composición bioquímica, índice de condición, ciclo reproductivo. June 2011, 217-226, Barcelona (Spain) Scientia Marina 75(2)

show abstract

Section: Introductionmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Reproductive activity and biochemical composition of the pullet carpet shell <i>Venerupis senegalensis</i> (Gmelin, 1791) (Mollusca: Bivalvia) from Ria de Aveiro (northwestern coast of Portugal)

Joaquim¹,

Matias²,

Matias³

et al. 2010

Sci. Mar.

View full text Add to dashboard Cite

show abstract

“…This decrease takes place during the spawning season (Gaspar and Monteiro 1998;Darriba et al 2005b) and should therefore be related to the loss of FAs contained in gametes (Taylor and Venn 1979;Ojea et al 2004;Hamdani and Soltani-Mazouni 2011). In fact, lipids are the second main component of marine bivalve eggs, accounting for 11.4-38.0 % of their dry weight (Bayne et al 1978;Pieters et al 1980;Lee and Heffernan 1991;Massapina et al 1999;Park et al 2003) or even 47.5 % DW (Davis and Wilson 1983). In the present study, biochemical analysis was performed on whole-body tissue.…”

Section: Fatty Acid Profile and Seasonalitymentioning

confidence: 92%

“…Yet, given the release of gametes and therefore the loss of protein-bound amino acids contained in eggs and sperm (Bressan and Marin 1985;Tarnowska et al 2009;Hamdani and SoltaniMazouni 2011), a decrease in P AA content would be expected to occur. In fact, marine bivalve eggs are mainly composed of proteins, with these compounds accounting for 32.9-74.0 % of their dry weight (Bayne et al 1978;Pieters et al 1980;Lee and Heffernan 1991;Massapina et al 1999;Park et al 2003). Moreover, pod razor clam eggs are relatively large (i.e., 99.3 ± 3.2 lm in diameter; da Costa 2009) and the egg mass in adult bivalve females was shown to reach 26.8-38.9 % of the individuals total dry weight (Park et al 2003;Park and Choi 2004).…”

Section: Amino Acid Profile and Seasonalitymentioning

confidence: 99%

Temporal dynamics of amino and fatty acid composition in the razor clam Ensis siliqua (Mollusca: Bivalvia)

et al. 2014

View full text Add to dashboard Cite

Few studies have been conducted on the temporal dynamics of both amino acid (AA) and fatty acid (FA) profiles in marine bivalves. We investigated the seasonal variation of these compounds in the pod razor clam Ensis siliqua in relation to food availability, salinity, water temperature and reproductive cycle. AA content varied between 46.94 and 54.67 % dry weight (DW), and the AAs found in greater quantity were glutamic acid, glycine and aspartic acid. FA content varied between 34.02 and 87.94 mg g -1 DW and the FAs found in greater quantity were 16:0 and 22:6n-3. Seasonal trends were observed for AAs and FAs. FAs increased with gametogenesis and decreased with spawning while AA content increased throughout spawning. The effect of increasing temperature and high food availability during the spawning season masked the loss of AAs resulting from gamete release. Still, a comparatively greater increase in the contents of glutamic acid and leucine with spawning indicate their possible involvement in a post-spawning gonad recovery mechanism. A post-spawning decrease in 14:0, 16:0, 16:1n-7, 18:1n-7 and 18:1n-9 is indicative of the importance of these FAs in bivalve eggs. An increase in 18:3n-3, 18:4n-3, 20:1n-9 and 20:2n-6 during gametogenesis suggests their involvement in oocyte maturation. The FA 22:4n-6, while increasing with spawning, appears to play a role in post-spawning gonad recovery. Salinity did not have an effect on the AA composition. None of the environmental parameters measured had an effect on FA composition.

show abstract

“…Although microscope observations are unavoidable and useful to validate flow cytometry (FCM), assays based on microscope observations often have little predictive value and can be subjective according to each observer. Determinations of biochemical content (proteins, carbohydrates, and lipids) have been used as criteria of oocyte quality (2)(3)(4). More recently, proteomic profiles of oocytes were proposed as quality estimators (5,6).…”

mentioning

confidence: 99%

Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure

et al. 2014

View full text Add to dashboard Cite

The Pacific oyster Crassostrea gigas accounts for a large part of shellfish aquaculture production worldwide. Aspects of morphological and functional characteristics of oyster oocytes remain poorly documented, and traditional techniques, such as microscopic observations of shape or fertilization rate, are time and space consuming. The purpose of this study was to assess for the first time viability and reactive oxygen species (ROS) production of Pacific oyster oocytes using flow cytometry (FCM) and to apply this method to determine oocyte responses to in vitro exposure to the toxic dinoflagellate Alexandrium minutum. A culture of A. minutum caused a significant increase in oocyte ROS production, which gradually increased with the age of the culture, but viability was not affected. Effect of the supernatant of the same A. minutum culture did not cause any significant modifications of oocyte morphology, viability, or ROS level. This study confirmed that some oocyte cellular characteristics can be assessed using FCM techniques. V C 2014 International Society for Advancement of Cytometry Key terms Pacific oyster; gamete quality; ROS production; viability; flow cytometry; ecotoxicology THE Pacific oyster Crassostrea gigas has considerable commercial value worldwide, including in France. Oyster seed production relies upon gamete quality, fertilization success, and larval development. As gamete quality is the first step in the spatproduction sequence, it is necessary to assess the quality of gametes in broodstock oysters first and foremost.To date, bioassays to assess oocyte quality are limited, and those available are tedious and time-consuming. Oocyte quality can be described using morphological characteristics by microscopy (1). Although microscope observations are unavoidable and useful to validate flow cytometry (FCM), assays based on microscope observations often have little predictive value and can be subjective according to each observer. Determinations of biochemical content (proteins, carbohydrates, and lipids) have been used as criteria of oocyte quality (2-4). More recently, proteomic profiles of oocytes were proposed as quality estimators (5,6). All of these biochemical assays, however, are fairly tedious to perform and provide only retrospective information. Fertilization rate, hatching rate, and larval development are used as complementary end points to evaluate oocyte quality (7-10); however, as these processes depend also upon spermatozoa quality, variability and inconsistency from one experiment to another often are encountered.

show abstract

Oocyte and embryo quality in Crassostrea gigas (Portuguese strain) during a spawning period in Algarve, South Portugal

Cited by 53 publications

References 19 publications

Reproductive activity and biochemical composition of the pullet carpet shell <i>Venerupis senegalensis</i> (Gmelin, 1791) (Mollusca: Bivalvia) from Ria de Aveiro (northwestern coast of Portugal)

Reproductive activity and biochemical composition of the pullet carpet shell <i>Venerupis senegalensis</i> (Gmelin, 1791) (Mollusca: Bivalvia) from Ria de Aveiro (northwestern coast of Portugal)

Temporal dynamics of amino and fatty acid composition in the razor clam Ensis siliqua (Mollusca: Bivalvia)

Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure

Contact Info

Product

Resources

About