Ontology for Blood Group Phenotyping and ABO Discrepancy Screening

Trongratsameethong, Areerat; Somhom, Samerkae; Chawachat, Jakarin; Wita, Ratsameetip; Anukul, Nampeung; Sirikul, Chonticha

doi:10.1109/jcsse53117.2021.9493808

Cited by 1 publication

(3 citation statements)

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“…Blood group antigens are known as an expression of human individuality and in pathological conditions a reduction of the expression of group A and B antigens is accompanied by an increase of the expression of precursor molecules. 14,28,29 Type III discrepancies were found in persons with modification of blood group type: a person of O blood group with anti-A (1:8 titre) and anti-B antibodies (1:4 titre) after allogeneic group AB transplantation for juvenile myelomonocytic leukaemia showed modification of blood group type: the forward typing demonstrated conversion to AB blood group (anti-A and anti-B reagents showed weak mixed-field agglutination). 17,30 Type III discrepancy was observed in persons with Hodgkin lymphoma, cord blood and plasma exchange with dextran or polyvinyl pyrrolidone.…”

Section: Discussionmentioning

confidence: 99%

“…The detection of weak antigens has been reported to be achieved by increasing the time of incubation, modifying the temperature of reaction, processing of erythrocytes with enzymes (stronger interaction of group A and B antigens with antibodies), molecular analysis, adsorption-elution methods. 14,24,25 Usually ABO discrepancies in forward and reverse typing are revealed at room temperature. Nevertheless, the authors underlined the necessity of using different temperatures in blood typing.…”

Section: Discussionmentioning

confidence: 99%

“…The use of different temperatures while incubation of erythrocytes with sera attracted atten-tion of many scientists due to the different reactivity of IgM and IgG antibodies at 4 °C and 37 °C. 14 Thus, antibody, IgM or IgG, may be of clinical significance if reactive at 37 °C. ABO discrepancies in persons with lymphoma and solid organ tumours have been resolved by elution and antibody screening at 37 °C and 40 °C.…”

Section: Introductionmentioning

confidence: 99%

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Resolving discrepancies in forward and reverse ABO blood group typing

Grigorovich,

Olexiyovich,

Solomonivna

et al. 2023

Scripta Medica

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Background/Aim: There is a need of investigation of high frequency of the cases of ABO discrepancy revealed by forward and reverse typing. The aim of the study was to reveal optimal methods for detection of weak subgroups of group A and B antigens on erythrocytes. Methods: Sixteen persons with A blood group and anti-B antibody adsorbing ability (weak Bel, Bm and B3 antigens), 5 persons with B blood group and anti-A antibody adsorbing ability (weak Ax antigen) and 2 persons with O blood group and anti-B antibody adsorbing ability (weak Bel antigen) were investigated as the cases of type II-III of ABO discrepancy and weak B antigen. Liquid chromatography, agglutination, inhibition of agglutination and adsorption reactions at 4 °C and 37 °C with prolonged incubation (12 hours) were used in the study. Results: The persons with A blood group revealed by forward typing at 4 °C and O blood group detected by reverse typing at 37 °C showed the ability of the erythrocytes to adsorb anti-B antibody. The persons with B blood group revealed by forward typing with presence of anti-B antibodies reactive at 37 °C showed the ability of erythrocytes to adsorb anti-A antibody. Prolonged incubation at reverse typing, adsorption-elution tests, inhibition of haemagglutination and liquid chromatography were used to define blood group specificity. Conclusion: Prolonged incubation during reverse typing at 4 °C and 37 °C, adsorption at 4 °C, heat elution and liquid chromatography might help to detect blood group type in cases of type II-III of ABO discrepancy.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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