Ontogeny of the interferon system in chickens

Karpala, Adam J.; Bagnaud-Baule, Audrey; Goossens, Kate E.; Lowenthal, John W.; Bean, Andrew G.D.

doi:10.1016/j.jri.2012.02.008

Cited by 25 publications

(22 citation statements)

References 25 publications

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“…Remarkably, the binding affinity between IFNAR2 and IFN is high, with a dissociation constant in nanomolar range, whereas the affinity between IFNAR1 and IFN is in the micromolar range [52], hypothetically because the demand for IFNAR1 in stable IFN-receptor complexes is greater than that for IFNAR2. Recent research shows that the fold change of IFNAR1 up-regulation is greater than IFNAR2 during development in chicken lung and spleen [53], supporting the above hypothesis. As shown in Figure 4C and D, the induction of IFNAR1 by ChIFNs is more prominent than that of IFNAR2, which is also consistent.…”

Section: Discussionsupporting

confidence: 54%

The Differential Antiviral Activities of Chicken Interferon α (ChIFN-α) and ChIFN-β are Related to Distinct Interferon-Stimulated Gene Expression

Yang

Meng

et al. 2013

PLoS ONE

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Chicken interferon α (ChIFN-α) and ChIFN-β are type I IFNs that are important antiviral cytokines in the innate immune system. In the present study, we identified the virus-induced expression of ChIFN-α and ChIFN-β in chicken fibroblast DF-1 cells and systematically evaluated the antiviral activities of recombinant ChIFN-α and ChIFN-β by cytopathic-effect (CPE) inhibition assays. We found that ChIFN-α exhibited stronger antiviral activity than ChIFN-β in terms of inhibiting the replication of vesicular stomatitis virus, Newcastle disease virus and avian influenza virus, respectively. To elucidate the mechanism of differential antiviral activities between the two ChIFNs, we measured the relative mRNA levels of IFN-stimulated genes (ISGs) in IFN-treated DF-1 cells by real-time PCR. ChIFN-α displayed greater induction potency than ChIFN-β on several ISGs encoding antiviral proteins and MHC-I, whereas ChIFN-α was less potent than ChIFN-β for inducing ISGs involved in signaling pathways. In conclusion, ChIFN-α and ChIFN-β presented differential induction potency on various sets of ISGs, and the stronger antiviral activity of ChIFN-α is likely attributed to the greater expression levels of downstream antiviral ISGs.

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Section: Discussionsupporting

confidence: 54%

The Differential Antiviral Activities of Chicken Interferon α (ChIFN-α) and ChIFN-β are Related to Distinct Interferon-Stimulated Gene Expression

Yang

Meng

et al. 2013

PLoS ONE

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“…TOC are considered as a valid model for the investigation of innate immune response parameters as well as viral replication, and furthermore allow comparison between tissue of turkey and chicken origin (Sekellick et al, 1990;Karpala et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Avian metapneumovirus infection of chicken and turkey tracheal organ cultures: comparison of virus–host interactions

Hartmann¹,

Sid²,

Rautenschlein³

2015

Avian Pathology

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Avian metapneumovirus (aMPV) is a pathogen with worldwide distribution, which can cause high economic losses in infected poultry. aMPV mainly causes infection of the upper respiratory tract in both chickens and turkeys, although turkeys seem to be more susceptible. Little is known about virus-host interactions at epithelial surfaces after aMPV infection. Tracheal organ cultures (TOC) are a suitable model to investigate virus-host interaction in the respiratory epithelium. Therefore, we investigated virus replication rates and lesion development in chicken and turkey TOC after infection with a virulent aMPV subtype A strain. Aspects of the innate immune response, such as interferon-α and inducible nitric oxide synthase mRNA expression, as well as virus-induced apoptosis were determined. The aMPV-replication rate was higher in turkey (TTOC) compared to chicken TOC (CTOC) (P < 0.05), providing circumstantial evidence that indeed turkeys may be more susceptible. The interferon-α response was down-regulated from 2 to 144 hours post infection in both species compared to virus-free controls (P < 0.05); this was more significant for CTOC than TTOC. Inducible nitric oxide synthase expression was significantly up-regulated in aMPV-A-infected TTOC and CTOC compared to virus-free controls (P < 0.05). However, the results suggest that NO may play a different role in aMPV pathogenesis between turkeys and chickens as indicated by differences in apoptosis rate and lesion development between species. Overall, our study reveals differences in innate immune response regulation and therefore may explain differences in aMPV -A replication rates between infected TTOC and CTOC, which subsequently lead to more severe clinical signs and a higher rate of secondary infections in turkeys.

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“…Proteins 3ab and 5ab have recently been associated with the chicken IFN response to IBV infection in cell culture [19][20][21]. However, the ability to induce IFN as well as the sensitivity to the action of IFN is only fully functional during the third and last week of embryonic development [32,33] and develops in ECEs with gestational age [33]. This might explain the limited differences in growth kinetics of the rIBV deletion mutants in eight-day-old embryonated SPF chicken eggs.…”

Section: Discussionmentioning

confidence: 99%

Recombinant live attenuated avian coronavirus vaccines with deletions in the accessory genes 3ab and/or 5ab protect against infectious bronchitis in chickens

Beurden¹,

Berends²,

Krämer-Kühl³

et al. 2018

Vaccine

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Avian coronavirus infectious bronchitis virus (IBV) is a respiratory pathogen of chickens, causing severe economic losses in poultry industry worldwide. Live attenuated viruses are widely used in both the broiler and layer industry because of their efficacy and ability to be mass applied. Recently, we established a novel reverse genetics system based on targeted RNA recombination to manipulate the genome of IBV strain H52. Here we explore the possibilities to attenuate IBV in a rational way in order to generate safe and effective vaccines against virulent IBV (van Beurden et al., 2017). To this end, we deleted the nonessential group-specific accessory genes 3 and/or 5 in the IBV genome by targeted RNA recombination and selected the recombinant viruses in embryonated eggs. The resulting recombinant (r) rIBV-Δ3ab, rIBV-Δ5ab, and rIBV-Δ3ab5ab could be rescued and grew to the same virus titer as recombinant and wild type IBV strain H52. Thus, genes 3ab and 5ab are not essential for replication in ovo. When administered to one-day-old chickens, rIBV-Δ3ab, rIBV-Δ5ab, and rIBV-Δ3ab5ab showed reduced ciliostasis as compared to rIBV H52 and wild type H52, indicating that the accessory genes contribute to the pathogenicity of IBV. After homologous challenge with the virulent IBV strain M41, all vaccinated chickens were protected against disease based on reduced loss of ciliary movement in the trachea compared to the non-vaccinated but challenged controls. Taken together, deletion of accessory genes 3ab and/or 5ab in IBV resulted in mutant viruses with an attenuated phenotype and the ability to induce protection in chickens. Hence, targeted RNA recombination based on virulent IBV provides opportunities for the development of a next generation of rationally designed live attenuated IBV vaccines.

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Ontogeny of the interferon system in chickens

Cited by 25 publications

References 25 publications

The Differential Antiviral Activities of Chicken Interferon α (ChIFN-α) and ChIFN-β are Related to Distinct Interferon-Stimulated Gene Expression

The Differential Antiviral Activities of Chicken Interferon α (ChIFN-α) and ChIFN-β are Related to Distinct Interferon-Stimulated Gene Expression

Avian metapneumovirus infection of chicken and turkey tracheal organ cultures: comparison of virus–host interactions

Recombinant live attenuated avian coronavirus vaccines with deletions in the accessory genes 3ab and/or 5ab protect against infectious bronchitis in chickens

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