Ontogeny of the Drosophila larval hematopoietic organ, hemocyte homeostasis and the dedicated cellular immune response to parasitism

Krzemień, Joanna; Crozatier, Michèle; Víncent, Alaín

doi:10.1387/ijdb.093053jk

Cited by 57 publications

(56 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies on hemocytes lineage evoque two possible origins to the lamellocytes: they could differentiate from either prohemocytes or plasmatocytes (Krzemien et al, 2010). We suggest that in D. affinis and D. obscura, the pseudopodocytes could represent an intermediate form or stage between plasmatocytes and lamellocytes.…”

Section: Resultsmentioning

confidence: 80%

Structural and functional characterization of pseudopodocyte, a shaggy immune cell produced by two Drosophila species of the obscura group

Havard¹,

Doury²,

Ravallec³

et al. 2012

Developmental & Comparative Immunology

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 80%

Structural and functional characterization of pseudopodocyte, a shaggy immune cell produced by two Drosophila species of the obscura group

Havard¹,

Doury²,

Ravallec³

et al. 2012

Developmental & Comparative Immunology

View full text Add to dashboard Cite

“…Pericardial cells separate the anterior lobes from the smaller posterior lobes. The Drosophila lymph gland is a model for insect and mammalian hematopoiesis 8,9 .…”

Section: Isolating and Fixing Host/parasite Structures Backgroundmentioning

confidence: 99%

An Introduction to Parasitic Wasps of <em>Drosophila</em> and the Antiparasite Immune Response

Small

Paddibhatla

Rajwani

et al. 2012

JoVE

View full text Add to dashboard Cite

Most known parasitoid wasp species attack the larval or pupal stages of Drosophila. While Trichopria drosophilae infect the pupal stages of the host (Fig. 1A-C), females of the genus Leptopilina (Fig. 1D, 1F, 1G) and Ganaspis (Fig. 1E) attack the larval stages. We use these parasites to study the molecular basis of a biological arms race. Parasitic wasps have tremendous value as biocontrol agents. Most of them carry virulence and other factors that modify host physiology and immunity. Analysis of Drosophila wasps is providing insights into how species-specific interactions shape the genetic structures of natural communities. These studies also serve as a model for understanding the hosts' immune physiology and how coordinated immune reactions are thwarted by this class of parasites.The larval/pupal cuticle serves as the first line of defense. The wasp ovipositor is a sharp needle-like structure that efficiently delivers eggs into the host hemocoel. Oviposition is followed by a wound healing reaction at the cuticle (Fig. 1C, arrowheads). Some wasps can insert two or more eggs into the same host, although the development of only one egg succeeds. Supernumerary eggs or developing larvae are eliminated by a process that is not yet understood. These wasps are therefore referred to as solitary parasitoids.Depending on the fly strain and the wasp species, the wasp egg has one of two fates. It is either encapsulated, so that its development is blocked (host emerges; Fig. 2 left); or the wasp egg hatches, develops, molts, and grows into an adult (wasp emerges; Fig. 2 right). L. heterotoma is one of the best-studied species of Drosophila parasitic wasps. It is a "generalist," which means that it can utilize most Drosophila species as hosts 1 . L. heterotoma and L. victoriae are sister species and they produce virus-like particles that actively interfere with the encapsulation response 2 . Unlike L. heterotoma, L. boulardi is a specialist parasite and the range of Drosophila species it utilizes is relatively limited 1

show abstract

“…Notably, the Drosophila larval hematopoietic organ, the lymph gland, provides a paradigm to reveal the relationships between the hematopoietic niche, the blood cell progenitors and their differentiated progenies (for a review, see Krzemien et al, 2010a). The lymph gland produces three mature blood cell types functionally related to mammalian myeloid cells: the plasmatocytes, the crystal cells and, upon some immune challenges, the lamellocytes (Lanot et al, 2001;Meister and Lagueux, 2003).…”

Section: Introductionmentioning

confidence: 99%

Dual role for Insulin/TOR signaling in the control of hematopoietic progenitor maintenance in Drosophila

et al. 2012

View full text Add to dashboard Cite

The interconnected Insulin/IGF signaling (IlS) and Target of Rapamycin (TOR) signaling pathways constitute the main branches of the nutrient-sensing system that couples growth to nutritional conditions in Drosophila. Here, we addressed the influence of these pathways and of diet restriction on the balance between the maintenance of multipotent hematopoietic progenitors and their differentiation in the Drosophila lymph gland. In this larval hematopoietic organ, a pool of stem-like progenitor blood cells (prohemocytes) is kept undifferentiated in response to signaling from a specialized group of cells forming the posterior signaling center (PSC), which serves as a stem cell niche. We show that, reminiscent of the situation in human, loss of the negative regulator of IIS Pten results in lymph gland hyperplasia, aberrant blood cell differentiation and hematopoietic progenitor exhaustion. Using site-directed loss- and gain-of-function analysis, we demonstrate that components of the IIS/TOR pathways control lymph gland homeostasis at two levels. First, they cell-autonomously regulate the size and activity of the hematopoietic niche. Second, they are required within the prohemocytes to control their growth and maintenance. Moreover, we show that diet restriction or genetic alteration mimicking amino acid deprivation triggers progenitor cell differentiation. Hence, our study highlights the role of the IIS/TOR pathways in orchestrating hematopoietic progenitor fate and links blood cell fate to nutritional status.

show abstract

Ontogeny of the Drosophila larval hematopoietic organ, hemocyte homeostasis and the dedicated cellular immune response to parasitism

Cited by 57 publications

References 86 publications

Structural and functional characterization of pseudopodocyte, a shaggy immune cell produced by two Drosophila species of the obscura group

Structural and functional characterization of pseudopodocyte, a shaggy immune cell produced by two Drosophila species of the obscura group

An Introduction to Parasitic Wasps of <em>Drosophila</em> and the Antiparasite Immune Response

Dual role for Insulin/TOR signaling in the control of hematopoietic progenitor maintenance in Drosophila

Contact Info

Product

Resources

About