Ontogeny of the antennal glands in the crayfish Astacus leptodactylus (Crustacea, Decapoda): immunolocalization of Na+,K+-ATPase

Abstract

The involvement of the antennal urinary glands in the ontogeny of osmoregulatory functions was investigated during the development of Astacus leptodactylus by measurements of hemolymph and urine osmolality in juvenile and adult crayfish and by the immunodetection of the enzyme Na+,K+-ATPase. In stage II juveniles, 1-year-old juveniles, and adults, all of which were maintained in freshwater, urine was significantly hypotonic to hemolymph. In adults, chloride and sodium concentrations were much lower in urine than in hemolymph. During embryonic development, Na+,K+-ATPase was detected by immunocytochemistry in ionocytes lining the tubule and the bladder, at an eye index (EI) of 220-250 microm, and in the labyrinth, at EI 350 microm. In all regions, immunofluorescence was mainly located at the basolateral side of the cells. No immunofluorescence was detected at any stage in the coelomosac. In late embryonic stages (EI 410-440 microm), in stage I juveniles, and in adults, strong positive immunofluorescence was found from the labyrinth up to and including the bladder. These results show that, as early as hatching, juvenile crayfish are able to produce dilute urine hypotonic to hemolymph. This ability originates from the presence of Na+,K+-ATPase in ion-transporting cells located in the labyrinth, the tubule, and the bladder of the antennal glands and constitutes one of the main adaptations of crayfish to freshwater.

Sodium uptake in different life stages of crustaceans: the water fleaDaphnia magnaStrauss

“…diffusion access distances), or that the mechanism(s) of Na + uptake is (are) fundamentally different in neonates and adults. Notably, ontogenetic changes seen in hyper-regulating crustaceans are generally associated with anatomical and/or structural changes in the ion-transporting cells and organs (Charmantier and CharmantierDaures, 2001;Charmantier et al, 2002;Cieluch et al, 2004;Khodabandeh et al, 2005a;Khodabandeh et al, 2005b).…”

“…In turn, physiological studies have clearly demonstrated that significant changes in the ability to cope with hypo-osmotic environments occur during the ontogenesis of the osmoregulatory organs in various crustacean groups, including cladocerans (daphnids), isopods, amphipods and decapods (crabs, lobsters, shrimps and crayfish) (Charmantier, 1998;Charmantier and Charmantier-Daures, 2001;Charmantier et al, 2002;Cieluch et al, 2004;Khodabandeh et al, 2005a;Khodabandeh et al, 2005b). The ontogenesis of the osmoregulation can occur at either the embryonic or the postembryonic phase (Charmantier and Charmantier-Daures, 2001).…”

Ultrastructure and osmoregulatory function of the kidney in larvae of the Persian sturgeon Acipenser persicus

“…The sections were pre‐incubated for 10 min in 0·01 mM Tween 20, 150 mM NaCI in 10 mM phosphate buffer, pH = 7·3, and then treated with 50 mM NH 4 Cl in phosphate‐buffered saline (PBS), pH = 7·3, for 5 min to mask the free aldehyde groups of the fixative. The sections were washed in PBS and incubated for 10 min with a blocking solution containing 1% bovine serum albumin (BSA) and 0·1% gelatin in PBS (Nebel et al , 2004, 2005; Khodabandeh et al , 2005 a , c ; Giffard‐Mena et al , 2006). Immunolocalization of the NKA was performed through immunofluorescence light microscopy using a mouse monoclonal antibody IgG α 5 (Hybridoma Bank, University of Iowa; http://dshb.biology.viowa.edu/) raised against the α ‐subunit of the chicken NKA (Takeyasu et al , 1988).…”

Osmoregulation in the Hawaiian anchialine shrimpHalocaridina rubra(Crustacea: Atyidae): expression of ion transporters, mitochondria-rich cell proliferation, and hemolymph osmolality during salinity transfers

“…This is notable as brachyuran gills are typically either respiratory or osmoregulatory in nature, with AgNO 3 staining typically confined to just the osmoregulating gills. For example, only the posterior gills undergo staining in C. sapidus and many other brachyurans (Copeland and Fitzjarrell, 1968), and while some lamellae in all gills show staining in FW crayfish, it is localized to the central filaments of each gill (Dickson et al, 1991;Khodabandeh et al, 2005). AgNO 3 staining of larval H. rubra revealed that ion-transporting gills develop at late zoeal stages (i.e.…”