Ontogeny of bile acid transport in brush border membrane vesicles from rat ileum

Moyer, M. Susan; Heubi, James E.; Goodrich, Anita L; Balistreri, William F.; Suchy, Frederick J.

doi:10.1016/0016-5085(86)90384-7

Cited by 41 publications

(24 citation statements)

References 28 publications

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“…Using this model, the apparent K m and V max values for taurocholate uptake at initial rates (i.e., 10 s) were 209Ϯ45 M and 1.23Ϯ0.14 nmol/ mg/10 s, respectively. Comparison of our kinetic data to pub- lished studies for bile acid transport in BBMV derived from distal ileum (22,28,26) and kidney (26) is shown in Table I. Overall, the kinetic parameters of taurocholate transport by cholangiocyte apical vesicles are comparable to published data using BBMV from distal ileum (22,28).…”

Section: Discussionsupporting

confidence: 76%

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Rat cholangiocytes absorb bile acids at their apical domain via the ileal sodium-dependent bile acid transporter.

Lazaridis¹,

et al. 1997

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Section: Discussionsupporting

confidence: 76%

“…Ileal brush border membrane vesicles (BBMV) were prepared as described (22). Crude, apical, and basolateral plasma membranes were prepared from NRC as described (17).…”

Section: Reverse Transcriptase Pcr (Rt-pcr)mentioning

confidence: 99%

Rat cholangiocytes absorb bile acids at their apical domain via the ileal sodium-dependent bile acid transporter.

Lazaridis¹,

et al. 1997

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“…Animals were killed by decapitation, and a 20-cm segment of terminal ileum and a 15-cm segment of proximal jejunum were obtained. Tissues were homogenized and BBMV were prepared as previously described (10). Sucrase levels were measured in ileal and jejunal homogenates.…”

Section: Animalsmentioning

confidence: 99%

“…Brush-border membrane protein enrichment was assayed using leucine aminopeptidase as a marker (12). Intravesicular volumes were estimated by measuring the uptake of L-['HI alanine into BBMV at equilibrium (60 min) in the presence of a 100-mM sodium or potassium thiocyanate gradient (10). Protein levels were measured by the method of Lowry et al (1 7).…”

Section: Animalsmentioning

confidence: 99%

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The Effects of Bile Acid Feeding on the Development of Ileal Bile Acid Transport

et al. 1993

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ABSTRACT. Sodium-dependent bile acid upta!te is developmentally regulated in the rat ileum. Transport activity is abruptly expressed on postnatal d 17, although the mechanisms controlling this expression are poorly understood. Changes in bile salt metabolism and hepatic transport result in a marked increase in bile flow before postnatal d 17, and thus this study examined the effects of bile salt feeding on the development of ileal bile acid transport. Twelve-d-old rat pups were gavage-fed saline, taurocholate, or mannitol on a daily basis for 3 d. Sodium-dependent bile acid transport was studied by rapid filtration using ileal brush-border membrane vesicles prepared from the various experimental groups. Taurocholate feeding resulted in precocious development of sodium-dependent bile acid transport and induction of sucrase activity. Mannitol feeding, used as a control for the effects of diarrhea-induced stress, resulted in similar sucrase activity, yet sodium-dependent bile acid transport was induced to only half the level observed in taurocholate-fed animals (3.2 + 1.6 versus 6.9 + 2.0 pmol/mg protein145 s, p < 0.001). Serum corticosterone levels were similar in the mannitol-and taurocholate-fed animals (3.8 + 1.3 versus 4.6 + 1.8 yg/dL). Both feedings lead to histologic maturation of the ileum, with a more pronounced effect in the taurocholate-fed pups. Bile salt feeding induces precocious expression of ileal bile acid transport, apparently by both diarrhea-induced stress and a bile salt-specific effect. (Pediatr Res 33: 221-224,1993) Abbreviations BBMV, brush-border membrane vesicles

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Ontogenic and glucocorticoid-accelerated expression of rat 14 kDa bile acid-binding protein

et al. 1996

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Background A 14 kDa protein is a major rat ileal cytosolic bile acid‐binding protein (14 kDa I‐BABP). This report describes the normal and glucocorticoid‐accelerated postnatal expression of 14 kDa I‐BABP. Methods 14 kDa I‐BABP and its mRNA were detected by antiserum and antisense cRNA probe, respectively, in ileum and ovary from 7‐, 14‐, 21‐, 28‐, and 35‐day‐old rats. Results A positive histochemical reaction with 14 kDa I‐BABP antiserum was found in cytosol of ileal enterocytes and ovarian luteal cells at 21 and 35 days of age, respectively. Likewise, Northern blot analysis indicated that the cRNA probe hybridized to a single transcript of 500 bp in total cellular RNA prepared from the ileum of 21‐day‐old and ovary of 35‐day‐old rats. Corticosteroid treatment resulted in a histochemical reaction in ileum of 14‐day‐old animals as compared to the appearance of this reaction in 21‐day‐old control littermates. Similarly, Northern blot analysis detected the earlier appearance of mRNA levels in corticosteroid‐treated animals (11 days vs. 17 days in control animals). Conclusions 14 kDa I‐BABP and its mRNA are normally expressed in rat ileum at 17 days of postnatal life. 14 kDa I‐BABP is expressed considerably later in rat ovary. Corticosteroid treatment results in precocious expression of ileal 14 kDa I‐BABP. © 1996 Wiley‐Liss, Inc.

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Ontogeny of bile acid transport in brush border membrane vesicles from rat ileum

Cited by 41 publications

References 28 publications

Rat cholangiocytes absorb bile acids at their apical domain via the ileal sodium-dependent bile acid transporter.

Rat cholangiocytes absorb bile acids at their apical domain via the ileal sodium-dependent bile acid transporter.

The Effects of Bile Acid Feeding on the Development of Ileal Bile Acid Transport

Ontogenic and glucocorticoid-accelerated expression of rat 14 kDa bile acid-binding protein

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