Ontogeny of B-lymphocyte differentiation induced by pokeweed mitogen

Wu, Lizhi; Blanco, Amalio; Cooper, Max D.; Lawton, Alexander R.

doi:10.1016/0090-1229(76)90026-x

Cited by 71 publications

(23 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding by was quickly confirmed by other investigators who also demonstrated the obligatory requirement for T cells in the culture Waldman et al, 1974;Janossy & Greaves, 1975;Keightley, Cooper & Lawton, 1976;Wu et al, 1976;Saxon, Stevens & Ashman, 1977;Fauci & Ballieux, 1979. It was also shown that the circulating human B lymphocytes could be identified by the presence of smIg in high density (Coombs, Feinstein & Wilson, 1969;Raff, 1970;Pernis, Forni & Amante, 1970;Rabellino et al, 1971;Kincade, Lawton & Cooper, 1971;Froland, Natvig & Berdal, 1971;Grey, Rabellino & Pirofsky, 1971), that PWM induced transformation and differentation ofthe B cells (Wu et al, , 1976Keightley et al, 1976), and that these B cells are lacking in individuals presenting with the Bruton (or X-linked) agammaglobulinaemia syndrome Grey et al, 1971;Cooper & Lawton, 1972;Aiuti, Fontana & Gatti, 1973), thus establishing these cells as the immunoglobulin-synthesizing cells. However, the B cells require the presence of monocytes (Gerrard & Fauci, 1981;Rosenberg & Lipsky, 1981;Shin & Choi, 1981;Theile & Lipsky, 1982) and T cells (Keightley et al, 1976;Saxon et al, 1977) in the cultures for immunoglobulin synthesis/secretion to take place.…”

Section: Discussionsupporting

confidence: 74%

Cells and mediators which participate in immunoglobulin synthesis by human mononuclear cells. II. The mechanism of null cell participation in immunoglobulin synthesis and secretion by B cells

Richter

Taylor

Macphail

et al. 1990

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARYImmunoglobulins were synthesized and secreted by human B cells cultured with T cells with receptors for FcM (TM) helper cells, monocytes, null cells and PWM for 7 days. Immunoglobulin synthesis did not take place if the null cells were omitted from the cultures irrespective ofthe duration ofthe culture period. Null cells incorporated into the cultures at only 25% of their optimal concentration did not affect immunoglobulin synthesis markedly by the cultured B cells. However, the number of B cells in the culture could not be diluted without an accompanying marked reduction in immunoglobulin synthesis. The B cells synthesized and secreted significant quantities of immunoglobulin even when the null cells were added as late as day 6 ofthe 7-day culture whereas no or very little immunoglobulin was synthesized if the B cells were not present from the beginning of the 7-day culture. It was demonstrated that cultured null cells do not transform into B cells and do not attain their immunoglobulin-synthesizing function. Furthermore, cultured B cells do not transform into null cells and do not attain their helper function. The null cells can also be distinguished from the B cells on the basis of cell-surface markers, receptors, and blastogenic responsiveness to phytomitogens. It is concluded that (i) the human circulating B cells require the null cells, in addition to the TM cells, monocytes and PWM, in culture in order to synthesize and secrete immunoglobulin; (ii) the null cell signal that stimulates immunoglobulin synthesis and secretion by the B cells is probably the last signal following the T^ helper cell, monocyte and PWM signals received by the B cells; and (iii) the null cells and the B cells constitute distinct lineages of cells.

show abstract

Section: Discussionsupporting

confidence: 74%

Cells and mediators which participate in immunoglobulin synthesis by human mononuclear cells. II. The mechanism of null cell participation in immunoglobulin synthesis and secretion by B cells

Richter

Taylor

Macphail

et al. 1990

Clinical and Experimental Immunology

View full text Add to dashboard Cite

show abstract

“…many mitogens. PWM drives primarily memory B cells and generates minimal Ig producing cells from neonatal B and T cells (4)(5)(6)(7)(8)(9). EBV and the combination ofSA and adult TF stimulate some Ig secretion, but the response is small and limited to IgM (7,8,14).…”

Section: Methodsmentioning

confidence: 99%

“…ing cells (ISC)' from neonatal lymphocytes (4)(5)(6)(7)(8). However, recent studies have demonstrated that the B cells induced to secrete Ig in response to PWM are largely surface IgD-negative, postswitch memory B cells (9-1 1).…”

Section: Introductionmentioning

confidence: 99%

Delineation of the functional capacity of human neonatal lymphocytes.

1991

View full text Add to dashboard Cite

show abstract

“…In addition, when tested in vitro, cord blood lymphocytes appear deficient in their ability to differentiate into plasma cells when stimulated with pokeweed mitogen (PWM)1 (2)(3)(4)(5). Neonatal T cells are functionally mature in terms of their capacity to respond to mitogens by proliferation, and neonatal monocytes have achieved full activity as effectors in antibody-dependent cellular cytotoxicity (6,7).…”

Section: Introductionmentioning

confidence: 99%

B Cell Differentiation and Immunoregulatory T Cell Function in Human Cord Blood Lymphocytes

Tosato¹,

Magrath²,

Koski³

et al. 1980

J. Clin. Invest.

113

View full text Add to dashboard Cite

A B S T R A C T The functional maturity of T and B lymphocyte populations from human newborns was evaluated using a reverse hemolytic plaque assay to detect immunoglobulin-secreting cells generated in in vitro cultures stimulated with pokeweed mitogen (PWM), a T cell-dependent polyclonal activator, and the Epstein-Barr virus (EBV), a T cell-independent B cell activator. Cord blood lymphocytes failed to produce immunoglobulin in response to PWM, but did respond with immunoglobulin synthesis to stimulation with EBV. Co-culture experiments demonstrated that cord blood T cells would inhibit immunoglobulin production by adult cells stimulated with PWM, but not with EBV. Cord blood T cells did suppress immunoglobulin production by cord blood B cells when stimulated with a mixture of EBV and PWM, indicating that cord blood, in contrast to adult blood, contains a population of suppressor T cell precursors that are easily activated by PWM. Irradiation of the cord blood T cells with 2,000 rad eliminated the suppressor activity and revealed normal helper function for immunoglobulin (Ig) G, A, and M when these T cells were co-cultured with adult B cells. Cord blood B cells co-cultured with adult T cells or irradiated cord blood T cells did produce immunoglobulin in response to PWM, but the response was significantly lower than that of adult B cells, and only IgM was produced in these cultures. These studies demonstrate that both the T and B cells of the human newborn have significant functional differences compared with the functions of T and B lymphocyte populations in adults. INTRODUCTION The immune status of the human neonate has been the subject of intense investigation for many years.

show abstract

Ontogeny of B-lymphocyte differentiation induced by pokeweed mitogen

Cited by 71 publications

References 22 publications

Cells and mediators which participate in immunoglobulin synthesis by human mononuclear cells. II. The mechanism of null cell participation in immunoglobulin synthesis and secretion by B cells

Cells and mediators which participate in immunoglobulin synthesis by human mononuclear cells. II. The mechanism of null cell participation in immunoglobulin synthesis and secretion by B cells

Delineation of the functional capacity of human neonatal lymphocytes.

B Cell Differentiation and Immunoregulatory T Cell Function in Human Cord Blood Lymphocytes

Contact Info

Product

Resources

About