Ontogeny of 3H-muscimol binding to membranes of chick forebrain

Stewart, Michael G.; Bourne, Rachel C.

doi:10.1007/bf00235999

Cited by 9 publications

(1 citation statement)

References 14 publications

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“…The preparation of brain membranes and radioligand binding assay were performed according to previous protocols (Stewart and Bourne, 1987;Yin and Lee, 1994). In brief, immediately after decapitation, the hippocampi of rats (n ϭ 20-30 for each control and drug-treated groups) were dissected on ice and homogenized in 50 mM Tris-HCl, pH 7.2.…”

Section: Radioligand Binding Assaymentioning

confidence: 99%

Effects of pentobarbital on the expression of GABAA receptor ?1 mRNA in the hippocampus: Differential responses of CA1 and CA3

Yin

Lee

1998

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Effects of barbiturates have been linked to the inhibitory GABAA receptor in the brain. The present study examines changes in the expression of GABAA receptor in the hippocampus of pentobarbital treated rat. Intraperitoneal pentobarbital injections were administered once daily for 9 days at an increasing dose schedule, 30 mg/kg at day 1-3, 60 mg/kg day 4-6, and 90 mg/kg day 7-9. Within each of the three dosage periods, the duration of sleep and extent of reduction in body temperature of the rats decreased with time. Two hours after the 9th injection, 3H-muscimol binding of the hippocampal homogenates of the animals showed that the maximal number of binding sites (Bmax), 10.2 +/- 1.6 pmol/mg protein, was not significantly greater than 9.5 +/- 1.2 of saline control, but strikingly about 7-fold control level of beta 1 mRNA was seen in the pyramidal cells of CA1 and CA2, as revealed by in situ hybridization analysis with digoxigenin-cRNA probes. However, when the rats were withdrawn from pentobarbital injection for 24 hours and 7 days, the Bmax of the hippocampi was lowered to 7.3 +/- 1.0 and 5.1 +/- 0.7, respectively, and the expression of beta 1 mRNA in CA1-2 returned toward control. The pentobarbital treatment did not significantly alter the affinity of the radioligand to the receptor in the hippocampus and the expression of beta 1 mRNA in CA3 and CA4. The results suggest the plasticity of the beta 1 mRNA in CA1-2 as well as differential involvement of CA1-2 and CA3-4 in response to the pentobarbital perturbation.

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Section: Radioligand Binding Assaymentioning

confidence: 99%

Effects of pentobarbital on the expression of GABAA receptor ?1 mRNA in the hippocampus: Differential responses of CA1 and CA3

Yin

Lee

1998

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Distribution of mu, delta, and kappa opioid receptor binding sites in the brain of the one‐day‐old domestic chick (Gallus domesticus): An in vitro quantitative autoradiographic study

Csillag

Bourne

Stewart

1990

J of Comparative Neurology

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Three highly specific opioid ligands--[D-Ala2,Gly-ol]-enkephalin (DAGO) for mu (mu) receptor sites, [D-Pen2,D-Pen5]-enkephalin (DPDPE) for delta (delta) sites, and U-69593 for kappa (kappa) sites--were used to determine the regional distribution of the three major subtypes of opioid receptor binding sites in the brains of 1-day-old domestic chicks by the technique of quantitative receptor autoradiography. Whilst there was a degree of heterogeneity in the binding levels of each of the ligands, some notable similarities existed in the binding of the mu and kappa ligands in several forebrain regions, and in the optic tectum of the midbrain where mu and delta binding was very high. In the forebrain there was a high level of binding of mu and kappa ligands in the hyperstriatum, and for the mu ligand there was a very distinct lamination of binding sites in hyperstriatum accessorium, intercalatum supremum, dorsale and ventrale. Levels of binding of the mu and kappa ligands were also high in nucleus basalis, and (for mu only) in the neostriatum. The distribution of binding of the delta specific ligand in the forebrain showed marked differences to that of mu and kappa, being particularly low in the hyperstriatum and neostriatum. Very high levels of labelling of delta binding sites were, however, found in the nucleus rotundus. Binding of the three ligands was generally low or absent in the cerebellum and medulla, apart from a distinct labelling of the granule cell layer by the mu-ligand. A kinetic analysis was made of the binding of the three ligands to whole forebrain sections using scintillation counting methods.(ABSTRACT TRUNCATED AT 250 WORDS)

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Autoradiographic localization of γ-aminobutyric acid receptors in mouse barrel field

1987

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Ontogeny of 3H-muscimol binding to membranes of chick forebrain

Cited by 9 publications

References 14 publications

Effects of pentobarbital on the expression of GABAA receptor ?1 mRNA in the hippocampus: Differential responses of CA1 and CA3

Effects of pentobarbital on the expression of GABAA receptor ?1 mRNA in the hippocampus: Differential responses of CA1 and CA3

Distribution of mu, delta, and kappa opioid receptor binding sites in the brain of the one‐day‐old domestic chick (Gallus domesticus): An in vitro quantitative autoradiographic study

Autoradiographic localization of γ-aminobutyric acid receptors in mouse barrel field

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