Ontogenic development of antidiuretic hormone receptors in rat kidney: Comparison of hormonal binding and adenylate cyclase activation

Rajerison, Rabary; Butlen, Daniel; Jard, Serge

doi:10.1016/0303-7207(76)90061-7

Cited by 88 publications

(32 citation statements)

References 28 publications

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“…In medullary cells, on the other hand, basal and forskolin-stimulated cAMP production per mg protein were similar in the two age groups. Similar findings have been reported in renal membrane fractions from 2-to 48-d-old rats (26). The high adenylate cyclase activity may be involved in the rapid cell growth in the cortex at this time.…”

Section: Discussionsupporting

confidence: 88%

The Effect of Dopamine on Adenylate Cyclase and Na+, K+-ATPase Activity in the Developing Rat Renal Cortical and Medullary Tubule Cells

et al. 1993

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ABSTRACT. Dopamine has an age-dependent natriuretic and diuretic effect. W e have investigated the ontogeny of the dopamine response on adenylate cyclase activity and Na+,K+-ATPase activity in two different cell populations in the infant (10-d-old) and the adult (40-d-old) rat kidney. Basal-and forskolin-stimulated adenylate cyclase activity in tubular suspensions of renal cortex was 5.4-fold ( p < 0.05) higher in the 10-d-old rats than in the 40-d-old rats but unchanged between the ages in a suspension of medullary tubules. The dopamine-1-specific agonist fenoldopam did not stimulate adenylate cyclase activity in the cortical cells from 10-d-old rats but did stimulate activity 51 f 16% ( p < 0.05) in the 40-d-old rats. In the medullary suspension, fenoldopam stimulated adenylate cyclase activity by 43.5 f 5% ( p < 0.001) in the 10-d-old rats and by 32.0 f 7% ( p < 0.01) in the 40-d-old rats. In the isolated proximal convoluted tubule, dopamine inhibited Na+,K+-ATPase activity in both the 10-d-old (34 f 3 % , p < 0.001) and 40-d-old rats (44 f 7%, p < 0.001). In contrast, in the medullary thick ascending limb of Henle, inhibition of Na+,K+-ATPase activity by fenoldopam was more pronounced in the 10-d-old (56 f 6%. p < 0.001) than in the 40-d-old rat (33 f 670, p < 0.001). In summary, the renal tubular effects of dopamine on adenylate cyclase and Na+,K+-ATPase activity change during postnatal development in a cell-specific manner. (Pediatr Res 34: 308-31 1, 1993) Abbreviations DA,, dopamine-1 PCT, proximal convoluted tubule mTAL, medullary thick ascending limb of Ilenle Na+,K+-ATPase, sodium, potassium adenosine triphosphatase DMEM, Dulbecco's modified Eagle's medium Intrarenally formed dopamine acts on tubular receptors to reduce sodium reabsorption (1-4). In the mTAL, dopamine inhibits Na+,K+-ATPase activity via activation of the DAI receptor, increase in adenylate cyclase activity, activation of CAMPdependent protein kinase, and activation of the dopamine-and CAMP-regulated protein phosphatase inhibitor DARPP-32 (5-7). In the PCT, Na+,K+-ATPase activity is inhibited by a synergistic effect of DAl and DA2 receptor agonists (8). 3(The natriuretic effect of dopamine has been demonstrated in the neonate (9), but the sensitivity to the hormone may be different in the adult and infant kidneys. In the experimental situation, several studies have reported an absent or weak renal response to dopamine in young animals (10-13). In this study on infant and adult rats, we show that the renal cffects of dopamine on adenylate cyclase and Na+,K+-ATPase activity change during postnatal development in a cell-specific manner. MATERIALS AND METHODSMale Sprague-Dawley rats aged 10-1 1 and 39-45 d were used. Body weights were 22-28 and 160-200 g. respectively. The adult rats were fed rrrl libilut)~ with synthetic rat food and had free access to tap water. The pups were kept with their dams.l'riy)uratiut~ qf tlrblrlur slr.spcnsion.s. For each experiment, material from two kidneys from 40-d-old and 12 kidneys from 10-d-old rats ...

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Section: Discussionsupporting

confidence: 88%

The Effect of Dopamine on Adenylate Cyclase and Na+, K+-ATPase Activity in the Developing Rat Renal Cortical and Medullary Tubule Cells

et al. 1993

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“…This concept was strongly supported by findings on membrane preparations of newborn rats in vitro which demonstrated that the activity of the ADH dependent adenylate-cyclase is reduced in comparison to the adult state (19,21). Hence, it is possible that, in addition to the immaturity of the countercurrent system and the parameters mentioned above, a lack of intracellular c-AMP formation as a consequence of the reduced enzyme activity could be responsible, at least in part, for the diminished concentrating capacity of the newborn kidney.…”

Section: Speculationmentioning

confidence: 77%

“…This phenomenon may be explained by two observations made in vitro: 1) On membrane preparations of the medulla of young rats and rabbits (19,21), the activity of renal adenylate-cyclase was found to be reduced in the neonatal state in comparison to the adult state.…”

Section: Discussionmentioning

confidence: 98%

Effect of Antidiuretic Hormone Upon Urinary Concentrating Ability and Medullary c-AMP Formation in Neonatal Piglets

et al. 1979

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SummaryIn 16 anesthetized piglets, the effects of I-deamino-8-D-arginine-vasopressin (DDAVP) upon urinary flow rate, relative urinary osrilolarity (U/P,,,,,,I), fractional water excretion (U/Pl,), fractional urca excretion (U/P,,,.,,/U/PI,), renal adenosine 3',5'-cyclic nlonophosphate (c-AMP) formation rate, and medullary c-AMP content were studied during the first 6 days of life. Glomerular filtration rate (GFR) was 0.19 + 0.09 ml/min/g kidney wt (means + SD) on day 1 and increased to 0.51 + 0.11 ml/min/g kidney wt on day 6. It was not affected by the application of 20 pg DDAVP/ kg body wt. On day 1, U/P,,,,,1(1.81 + 0.29), urinary flow rate (5.36 + 1.49 pl/n~in/g kidney wt), U/PI, (31.67 + 6.10) and U/ P,,,.,,/U/PI, (0.84 + 0.12) were not affected by DDAVP application. Fro111 day 2 onward, the reponse of these variables to application of DDAVP increased progressively. On day 6, the following responses were observed: Urinary flow rate decreased from 13.85 f. 7.02 pl/nlin/g kidney wt before DDAVP to 5.07 ? 2.19 pl/min/ g kidney wt after DDAVP. U/P,,,,I raised from 1.24 + 0.27 before DDAVP to 2.64 ? 0.17 after DDAVP. U/PI, increased fro111 36.85 + 13.11 before DDAVP to 109.39 + 22.00 after DDAVP. U/P,,,,,/U/P,, decreased from 0.75 ? 0.05 before DDAVP to 0.55 + 0.04 after DDAVP. These data indicate that the snlall solute permeability of the medullary collecting ducts becon~es increasingly sensitive to antidiuretic hormone (ADII) during this period. The increase in responsiveness of the urinary concentrating system with age was paralleled by the effect of DDAVP on renal c-AMP fornlation rate. In the diuretic control state, no correlation between renal c-AMP fornlation rate and the number of days was observed. llowever, after the application of DDAVP, the nephrogenic fraction of renal c-AMP excretion rate increased from 10.58 + 4.49 pnlole/min/g kidney wt on day 1 to 21.61 + 8.10 pmole/ n~in/g kidney wt on day 6. Similar results were observed with the nledullary c-AMP content. In diuretic control animals which had riot received DDAVP, the medullary c-AMP content ranged between 636 ? 257 pnlole/g wet wt on day 1 and 622 + 75 pmole/g wet wt on day 6. IIowever, in animals which had been infused with DDAVP, niedullary c-AMP content increased from 684 + 274 pnrole/g wet wt on day 1 to 1536 ? 316 pmole/g wet wt on day 6. SpeculationThe unresponsiveness of the medullary adenylatecyclase to AD11 in the newborn kidney might restrict, in addition to other parameters, the renal concentrating ability during the neonatal period.It is generally acknowledged that the concentrating ability of the newborn kidney is reduced in comparison to that of the mature kidney. In the past, this phenomenon was predominately attributed to the structural immaturity of the renal medulla (2, 22), to the reduced G F R and to the anabolic state of the neonatal metabolism, which together result in low filtration-, reabsorption-, and excretion rate of urea (5, 6). It was argued that in spite of the effects of ADH on the permeability of the collecting d...

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“…Referente a hormona antidiuretica, se ha demostrado que las ratas recien nacidas tienen menor concentracion de receptores para dicha hormona en el nefron distal en comparacion con ratas adultas 32 . y recientemente Aperia y col. 33 han demostrado convincenteniente que los RNT pueden incrementar su excrecion acuosa frente a una ingesta aumentada de agua.…”

Section: Discusionunclassified

Excreción Urinaria de Calicreína en Recién Nacidos

L²,

H³

et al. 1986

Rev. chil. pediatr.

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Ontogenic development of antidiuretic hormone receptors in rat kidney: Comparison of hormonal binding and adenylate cyclase activation

Cited by 88 publications

References 28 publications

The Effect of Dopamine on Adenylate Cyclase and Na+, K+-ATPase Activity in the Developing Rat Renal Cortical and Medullary Tubule Cells

The Effect of Dopamine on Adenylate Cyclase and Na+, K+-ATPase Activity in the Developing Rat Renal Cortical and Medullary Tubule Cells

Effect of Antidiuretic Hormone Upon Urinary Concentrating Ability and Medullary c-AMP Formation in Neonatal Piglets

Excreción Urinaria de Calicreína en Recién Nacidos

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