Ontogenetic variation in the ecological stoichiometry of 10 fish species during early development

Abstract

The chemical composition and stoichiometry of vertebrate bodies changes greatly during ontogeny as phosphorus‐rich bones form, but we know little about variation among species during early development. Such variation is important because element ratios in animal bodies influence which element limits growth, and how animals contribute to nutrient cycling. We quantified ontogenetic variation from embryos through 2‐3 months of age in 10 species of fish in 6 different families, ranging in adult size from 73 to 720 mm length. We measured whole‐body concentrations (percentage of dry mass) and ratios of carbon (C), nitrogen (N) and phosphorus (P) as fish developed. We also quantified whole‐body concentrations of calcium (Ca), because Ca should reflect bone development, and RNA which can be a major pool of body P. To account for interspecific differences in adult size, we also examined how trends changed with relative size, defined as body length divided by adult length. Ontogenetic changes in body composition and ratios were relatively similar among species, and were more similar when expressed as a function of relative size, compared to age. Body P increased rapidly in all species (likely because of bone development) from embryos until individuals were ~5‐8% of adult size. Body N also increased, while body C, C:N, C:P and N:P all decreased over this period. Body Ca increased with development, but was more variable among species. Body RNA was low in embryos, increased rapidly in young larvae, then decreased as fish reached 5‐8% of adult size. After fish were about 5‐8% of adult size, changes in body composition were relatively slight for all elements and ratios. These results reveal a consistency in the dynamics of body stoichiometry during early ontogeny, presumably because of similar constraints on the allocation of elements to bones and other body pools. Because most changes occur when individuals are <1 month old (<10% of adult size for that species), early ontogenetic variation in body stoichiometry may be especially important for growth limitation of individuals and ecosystem‐level nutrient cycling.This article is protected by copyright. All rights reserved.

The vertebrate bone hypothesis: Understanding the impact of bone on vertebrate stoichiometry

“…nucleic acids) to primarily structural (e.g. Downs et al, 2023). Since bone has an N:P below 1, whole-body %P rapidly increases and whole-body N:P rapidly decreases as bones mineralize (e.g.…”

“…Since bone has an N:P below 1, whole-body %P rapidly increases and whole-body N:P rapidly decreases as bones mineralize (e.g. Downs et al, 2023;Luhring, 2013;McLeay et al, 2019;Pilati & Vanni, 2007). Because of bone's high nutrient content, strong P or Ca limitation can occur during bone development (Baeverfjord et al, 1998;Ogino & Takeda, 1976;Robinson et al, 1987;Stephens et al, 2017), especially since developing vertebrates simultaneously require P-rich RNA to fuel growth (Elser et al, 2003).…”