Ontogenetic shifts in selected body temperature and thermal tolerance of the tiger frog,<i>Hoplobatrachus chinensis</i>

Xiaoli, 樊晓丽 Fan; Huanzong, 雷焕宗 Lei; Zhihua, 林植华 Lin

doi:10.5846/stxb201202290271

Cited by 5 publications

(1 citation statement)

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“…The three kinds of feeds were then oven heated at 50 C for 2 h to completely volatilize the ethanol, and the feeds with letrozole concentration of 0 mg/g, 0.1 mg/g and 1 mg/g were prepared for later use. In previous studies, researchers have found that the body temperature preference for the growth and development of H. rugulosus tadpoles is 28.2 C (Fan, Lei & Lin, 2012). Another study found that the sex ratio was biased toward males at 30 C and that 100% masculinization occurred at 35 C (Fu, 2010), suggesting that high temperatures can make H. rugulosus tadpoles produce more male offspring.…”

Section: Animal Collection and Treatmentmentioning

confidence: 98%

The combined effects of temperature and aromatase inhibitor on metamorphosis, growth, locomotion, and sex ratio of tiger frog (Hoplobatrachus rugulosus) tadpoles

Tang

Chen

Lin

et al. 2020

PeerJ

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Background The tiger frog (Hoplobatrachus rugulosus) is widely raised by many farms in southern region of China as an economically edible frog. The growth, development, and sexual differentiation of amphibians are influenced by temperature and steroid hormone level. However, the problem of hormone residues is caused by the addition of exogenous hormones in frog breeding, it is worth considering whether non-sterol aromatase inhibitors can be used instead of hormones. Methods In our study, H. rugulosus tadpoles were subjected to two water temperatures (29 °C and 34 °C) and three letrozole concentrations in the feed (0, 0.1 and 1 mg/g) to examine the effects of temperature, aromatase inhibitor and their interaction on metamorphosis, locomotion, and sex ratios. A G-test and contingency table were used to analyze the metamorphosis rate of tadpoles and the survival rate of froglets after feeding for 90 days. A G-test was also used to analyze sex ratios in different treatment groups. Results Metamorphosis time and body size (snout–vent length, body mass and condition factor) were significantly different between the two temperature treatments. Metamorphosis time was longer and body size was increased at 29 °C compared to those at 34 °C. Letrozole concentration and the temperature × letrozole interaction did not affect these variables. The jumping distance of froglets following metamorphosis was positively associated with the condition factor; when controlling for condition factor, jumping distance was not affected by temperature, letrozole concentration and their interaction. Temperature and letrozole concentration also did not affect metamorphosis and survival rate. Sex ratio of the control group (0 mg/g letrozole) was 1:1 at 29 °C, but there were more males at 34 °C. The sex ratios of H. rugulosus treated with letrozole at 29 °C and 34 °C were significantly biased toward males, and male ratio increased as letrozole concentration increased. Furthermore, more males were produced at 34 °C than at 29 °C at each letrozole concentration.

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Section: Animal Collection and Treatmentmentioning

confidence: 98%