Ontogenetic expression of cell adhesion molecules: L-CAM is found in epithelia derived from the three primary germ layers

Timing and kinetics of E‐ to N‐cadherin switch during neurulation in the avian embryo

“…The mAb to mouse E-cadherin (clone 36/E-cadherin) directed against its cytoplasmic tail and crossreacting with chick E-cadherin (also known as L-CAM) was from BD Transduction Laboratories (San Diego, CA). This mAb produced the same staining as rabbit polyclonal antibodies to the external part of the L-CAM molecule (Thiery et al, 1984). The rabbit polyclonal antibody to chick fibronectin was described previously (Rovasio et al, 1983).…”

“…In the chick embryo, the neural plate, from which the entire central nervous system is formed, is initially a flat epithelium of cuboidal cells that express E-cadherin and not N-cadherin. At completion of neurulation, when the neural tube is formed and fully separated from the superficial ectoderm, neural epithelial cells exhibit their typical elongated, radially-oriented shape and constitute a pseudo-stratified epithelium in which E-cadherin has been totally replaced by N-cadherin (Thiery et al, 1984;Hatta et al, 1987;Duband et al, 1988;Detrick et al, 1990;Radice et al, 1997). Yet, neural epithelial cells are unable to undergo spontaneous EMT; instead, they tend to remain coherent, even in two-dimensional culture where they retain their organization as a hollow tube (Duband et al, 2009).…”

Zebrafish E‐cadherin: Expression during early embryogenesis and regulation during brain development

“…E-cadherin is essential for early embryogenesis in mouse (Larue et al, 1994;Riethmacher et al, 1995). Detailed analysis of E-cadherin expression in embryogenesis was described for mouse, chick, and Xenopus (Broders et al, 1993;Choi and Gumbiner, 1989;Hirai et al, 1989a,b;Levi et al, 1991;Shimamura and Takeichi, 1992;Shimamura et al, 1994;Takeichi, 1988;Thiery et al, 1984). E-cadherin expression in the ectoderm is downregulated during neurulation (Takeichi, 1988).…”

“…E-cadherin expression in the ectoderm is downregulated during neurulation (Takeichi, 1988). However, discrete regions of E-cadherin expression are activated in the developing and adult brain (Choi and Gumbiner, 1989;Shimamura and Takeichi, 1992;Shimamura et al, 1994;Thiery et al, 1984). The functional significance of neural E-cadherin expression is unclear.…”

“…In the mouse and chick but not in Xenopus, E-cadherin is expressed in the MHB (Choi and Gumbiner, 1989;Shimamura and Takeichi, 1992;Shimamura et al, 1994;Thiery et al, 1984). In zebrafish, mutations in the Pax2.1 gene (no isthmus or noi) and in the Fgf8 gene (acerebellar or ace) disrupt MHB development (Brand et al, 1996).…”