Ontogenetic Changes in Shell Microstructures in the Cold Seep-Associated Bivalve,<i>Conchocele bisecta</i>(Bivalvia: Thyasiridae)

Nishida, Kozue; Nakashima, Rei; Majima, Ryuichi; Hikida, Yoshinori

doi:10.2517/1342-8144-15.4.193

Cited by 10 publications

(7 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Through the use of novel analytical techniques, we were able to automate the recognition of microstructural entities as well as the morphometric analyses, which hitherto had to be performed manually or semi-automatically with manual corrections [89,93]. Previous morphometric studies of BMUs included only few SEM images representing relatively small shell areas (e.g., 19 images in [25], 64 images in [94]) in order to compensate for the excessively long analytical times.…”

Section: Plos Onementioning

confidence: 99%

Temperature-induced microstructural changes in shells of laboratory-grown Arctica islandica (Bivalvia)

Höche¹,

Walliser²,

Winter

et al. 2021

PLoS ONE

View full text Add to dashboard Cite

Bivalve shells are increasingly used as archives for high-resolution paleoclimate analyses. However, there is still an urgent need for quantitative temperature proxies that work without knowledge of the water chemistry–as is required for δ18O-based paleothermometry–and can better withstand diagenetic overprint. Recently, microstructural properties have been identified as a potential candidate fulfilling these requirements. So far, only few different microstructure categories (nacreous, prismatic and crossed-lamellar) of some short-lived species have been studied in detail, and in all such studies, the size and/or shape of individual biomineral units was found to increase with water temperature. Here, we explore whether the same applies to properties of the crossed-acicular microstructure in the hinge plate of Arctica islandica, the microstructurally most uniform shell portion in this species. In order to focus solely on the effect of temperature on microstructural properties, this study uses bivalves that grew their shells under controlled temperature conditions (1, 3, 6, 9, 12 and 15°C) in the laboratory. With increasing temperature, the size of the largest individual biomineral units and the relative proportion of shell occupied by the crystalline phase increased. The size of the largest pores, a specific microstructural feature of A. islandica, whose potential role in biomineralization is discussed here, increased exponentially with culturing temperature. This study employs scanning electron microscopy in combination with automated image processing software, including an innovative machine learning–based image segmentation method. The new method greatly facilitates the recognition of microstructural entities and enables a faster and more reliable microstructural analysis than previously used techniques. Results of this study establish the new microstructural temperature proxy in the crossed-acicular microstructures of A. islandica and point to an overarching control mechanism of temperature on the micrometer-scale architecture of bivalve shells across species boundaries.

show abstract

Section: Plos Onementioning

confidence: 99%

Temperature-induced microstructural changes in shells of laboratory-grown Arctica islandica (Bivalvia)

Höche¹,

Walliser²,

Winter

et al. 2021

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Moreover, the shell microstructures made by a single individual can differ, depending on the environmental conditions it experiences during biomineralization [ Carter , ]. For example, cyclical changes in the shell microstructure within a single shell layer have been observed in various taxa of Bivalvia [ Nishida et al ., ].…”

Section: Introductionmentioning

confidence: 99%

Thermal dependency of shell growth, microstructure, and stable isotopes in laboratory‐reared Scapharca broughtonii (Mollusca: Bivalvia)

Nishida

Suzuki

Isono

et al. 2015

Geochem Geophys Geosyst

Self Cite

View full text Add to dashboard Cite

We experimentally examined the growth, microstructure, and chemistry of shells of the bloody clam, Scapharca broughtonii (Mollusca: Bivalvia), reared at five temperatures (13, 17, 21, 25, and 298C) with a constant pCO 2 condition ($450 latm). In this species, the exterior side of the shell is characterized by a composite prismatic structure; on the interior side, it has a crossed lamellar structure on the interior surface. We previously found a negative correlation between temperature and the relative thickness of the composite prismatic structure in field-collected specimens. In the reared specimens, the relationship curve between temperature and the growth increment of the composite prismatic structure was humped shaped, with a maximum at 178C, which was compatible with the results obtained in the field-collected specimens. In contrast, the thickness of the crossed lamellar structure was constant over the temperature range tested. These results suggest that the composite prismatic structure principally accounts for the thermal dependency of shell growth, and this inference was supported by the finding that shell growth rates were significantly correlated with the thickness of the composite prismatic structure. We also found a negative relationship between the rearing temperature and d18 O of the shell margin, in close quantitative agreement with previous reports. The findings presented here will contribute to the improved age determination of fossil and recent clams based on seasonal microstructural records.

show abstract

“…Muscle scars in bivalves have a characteristic microstructure (myostracum; Stenzel, 1963;Kennedy et al, 1969;Lee et al, 2011) composed of aragonite prisms oriented perpendicular to the valve surface (Suzuki and Nagasawa, 2013). Because shells grow by accretion, inner shell layers are laid over the myostracum from earlier formed portions of the valve (Kennedy et al, 1969;Garcia-March et al, 2011;Lee et al, 2011;Nishida et al, 2011). If we assume that dissolution altered the valves from their outer surfaces inward, then we might predict that earlier precipitated myostracal layers would become exposed on dead shells, thereby lengthening adductor scars by shifting LMs that define their dorsal margins in a dorsal direction.…”

Section: Discussionmentioning

confidence: 99%

Live and Live-Dead Intraspecific Morphometric Comparisons as Proxies for Seagrass Stability in Conservation Paleobiology

et al. 2022

View full text Add to dashboard Cite

Comparisons of life and death assemblages are commonly conducted to detect environmental change, including when historical records of live occurrences are unavailable. Most live-dead comparisons focus on assemblage composition, but morphology can also vary in species with environmental variables. Although live-dead morphologic comparisons are less explored, their data could be useful as a proxy in conservation paleobiology. We tested the potential for geometric morphometric data from live-and dead-articulated Stewartia floridana (Bivalvia: Lucinidae) to serve as proxies for seagrass occurrence and stability. The study area is at the northern end of Pine Island in Charlotte Harbor, FL, United States, an estuarine system with substantial seagrass loss in the 20th century and subsequent partial recovery. The area sampled has had relatively stable seagrass occurrences since at least the early 2000s. Live and dead-articulated S. floridana samples were collected from two transects through a patchy seagrass meadow, with sampled sites ranging from bare sand to 100% seagrass cover. Dead-articulated specimens were also collected from three adjacent transects. Live S. floridana shape covaried significantly with seagrass taxonomic composition and percent cover at the time of collection based on two-block partial least squares analysis, although shape differences between seagrass end members (100% Halodule wrightii and 100% Syringodium filiforme) were not significant by multivariate analysis of variance (MANOVA). Instead, specimens from 100% H. wrightii had significantly greater Procrustes variance. Live S. floridana shape data placed in categories describing seagrass stability over 6 years prior to sampling (and reflecting sclerochronologic estimates of maximum longevity) differed significantly based on MANOVA. For live and dead S. floridana from the same transects, shape differed significantly, but allometric trends did not. In addition, patterns of morphologic variation tied to seagrass stability were detected in dead-articulated valve shape. Dead shells from adjacent transects differed significantly in shape and allometric trend from both live and dead specimens collected together. We infer that morphometric differences recorded fine-scale spatial and temporal patterns possibly tied to environmental change. Therefore, geometric morphometrics may be a powerful tool that allows for death assemblages to track seagrass distributions through time prior to systematic monitoring, including in areas under high anthropogenic stress.

show abstract

Ontogenetic Changes in Shell Microstructures in the Cold Seep-Associated Bivalve,Conchocele bisecta(Bivalvia: Thyasiridae)

Cited by 10 publications

References 23 publications

Temperature-induced microstructural changes in shells of laboratory-grown Arctica islandica (Bivalvia)

Temperature-induced microstructural changes in shells of laboratory-grown Arctica islandica (Bivalvia)

Thermal dependency of shell growth, microstructure, and stable isotopes in laboratory‐reared Scapharca broughtonii (Mollusca: Bivalvia)

Live and Live-Dead Intraspecific Morphometric Comparisons as Proxies for Seagrass Stability in Conservation Paleobiology

Contact Info

Product

Resources

About