Ontogenetic changes in RNA, DNA and protein contents of laboratory-reared Pacific bluefin tuna Thunnus�orientalis

Tanaka, Yosuke; Gwak, Woo-Seok; Tanaka, Masaru; Sawada, Yoshifumi; Okada, Tokihiko; Miyashita, Shigeru; Kumai, Hidemi

doi:10.1111/j.1444-2906.2007.01345.x

Cited by 37 publications

(34 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The decline of DNA concentration during development was also indicated by Buckley et al, (1999) and Fonseca et al (2006). In addition, in agreement with our results, Tanaka et al (2007) found for bluefin tuna larvae an increase in the total amount of DNA and RNA as development advanced, but the standardised RNA and protein contents per weight unit (mg/mg WW) showed steady concentrations during development. In the same study, the DNA content per unit weight began to decrease and maintained a low level from 21 DAH.…”

Section: Discussionsupporting

confidence: 80%

“…In the same study, the DNA content per unit weight began to decrease and maintained a low level from 21 DAH. At this time, the protein-DNA ratio showed a rapid increase associated with the rapid somatic growth in both length and weight, suggesting cell enlargement (Tanaka et al, 2007). Johnson and Hall (2004) also showed a decrease in hyperplasia in muscle cells in larvae larger than 20 mm in clupeid species, such as herring.…”

Section: Discussionmentioning

confidence: 94%

See 1 more Smart Citation

Tissue effect on RNA:DNA ratios of marine fish larvae

Olivar¹,

Diaz²,

Chı́charo³

2009

Sci. Mar.

View full text Add to dashboard Cite

SUMMARY:In some routine studies of larval condition based on RNA:DNA ratios, heads and/or guts are removed for further age and feeding analysis. Also, during capture larvae are often found with their eyes missing. In this work we analysed tissues effects (muscle, head, eye, gut and the whole larvae) on RNA:DNA ratios from different species (Sardina pilchardus, Engraulis encrasicolus, Atherina presbyter and Paralichthys orbignyanus) in different developmental stages and from different locations. For all species and development phases, it was shown that RNA:DNA ratios in the head were significantly lower than in muscle or gut. The comparison of RNA:DNA ratios of head with or without eyes did not show significant differences. In a comparison of results from muscle from two tail sections, RNA:DNA ratios were higher for the anterior section in Sardina pilchardus and Engraulis encrasicolus larvae. Caution is needed when RNA:DNA ratios are compared with other studies employing different dissection protocols. For the species studied we propose conversion factors to be applied to RNA:DNA ratio data when different body sections are analysed. We discuss the most appropriate tissues for assessing nutritional condition in fish larvae, based on trends in concentrations of nucleic acids and ratios during starvation experiments.Keywords: RNA:DNA, tissues, starvation, condition, Sardina pilchardus, Engraulis encrasicolus, Atherina presbyter, Paralichthys orbignyanus. RESUMEN: Efecto del tipo de tejido sobre los cocientes ARN:ADN en larvas de peces marinos. -En estudios de condición nutricional larvaria basados en la relación ARN:ADN, las cabezas y/o tubos digestivos suelen diseccionarse para posteriores estudios de edad o alimentación. Además, durante la captura algunas larvas pierden los ojos. En este trabajo analizamos el efecto de diferentes tejidos (músculo, cabeza, ojos y tubo digestivo) sobre la relación ARN:ADN en Sardina pilchardus, Engraulis encrasicolus, Atherina presbyter y Paralichthys orbignyanus, en diferentes estadios de desarrollo y en diversas regiones. En todas las especies la relación ARN:ADN en la cabeza fue significativamente menor que para el músculo o el tubo digestivo. La comparación de la relación ARN:ADN de la cabeza con o sin ojos no mostró diferencias significativas. Al comparar dos secciones de músculo de la cola en Sardina pilchardus y Engraulis encrasicolus se observó mayor ARN:ADN en la parte anterior que en la posterior. Es necesaria precaución cuando se comparan ARN:ADN entre estudios que emplean diferentes protocolos de disección. Proponemos factores de conversión para ser aplicados a las relaciones ARN:ADN cuando se analizan diferentes secciones del cuerpo. Se discute cuales son los tejidos más apropiados para determinar la condición nutricional de larvas de peces, basados en las concentraciones de ácidos nucleicos y sus cocientes obtenidos en experimentos de inanición.

show abstract

Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 94%

Tissue effect on RNA:DNA ratios of marine fish larvae

Olivar¹,

Diaz²,

Chı́charo³

2009

Sci. Mar.

View full text Add to dashboard Cite

show abstract

“…The increase after 20 DAH was due to the lower growth rate of body mass, as observed in blue fin tuna Thunnus orientalis (Temminck & Schlegel) (Tanaka et al, 2007). Based on previous observations (Park et al, 2008), the decline of protein concentration after 28 DAH was maybe due to a high rate of skeletal formation and cellular hydrolysis activity.…”

Section: Discussionmentioning

confidence: 91%

Ontogenetic changes in nucleic acid, protein contents, and growthof larval and juvenile Japanese flounder

Tong¹,

Bao²,

Tang³

et al. 2017

Turk J Zool

View full text Add to dashboard Cite

In order to estimate relationships among growth potential and biochemical indicators of Japanese flounder, RNA and DNA as well as protein indices were measured from 5 days after hatching (DAH) to 45 DAH. Results showed that the RNA/DNA ratio and protein/DNA ratio had obvious relations with instantaneous growth rate (G M ) and length-based instantaneous growth rate (G L ) during the premetamorphic period. Significant negative correlations among protein/DNA ratio and G M and G L were then observed during metamorphosis. During the postmetamorphic phase, the RNA/DNA ratio was positively correlated with G M and G L . Data indicated that body growth of Japanese flounder is mostly hyperplastic before 20 DAH, hypertrophic until 27 DAH, and hyperplastic until the end. In order to investigate the effect of diel periodicity on RNA/DNA ratio in larvae of the fed and starved groups, an experiment was conducted for 2 days. Results showed that the average RNA/DNA ratio in the daytime was higher than that in the dark and the ratio in fed larvae was higher compared with that in starved ones. In order to examine starvation effect on RNA/DNA ratio, experiments were conducted from 20 DAH to 27 DAH. Juveniles were divided into five groups: a fed group and groups starved 1, 2, 3, and 4 days followed by refeeding. Results showed that the RNA/DNA ratio increased in the fed group and declined continuously in the starved group. After refeeding, the recovery of the RNA/DNA ratio was observed for 1-day, 2-day, and 3-day starved treatments but was not found for the 4-day starved treatment.

show abstract

“…It was reported that white muscle of yellowfin tuna Thunnus albacores contains a considerable amount of inosinic acid among the amino acid-related compounds [34]. It was also reported that the RNA/DNA ratio in PBT drastically increases at 21 dph, suggesting that active RNA and subsequent cell proliferation take place in PBT at this stage [35]. Dietary IMP could be an important component that allows cell proliferation in PBT during the stages of fast growth.…”

Section: Discussionmentioning

confidence: 96%

Improvement in the feeding activity, early growth and survival of Pacific bluefin tuna Thunnus orientalis larvae fed a casein peptide-based microdiet supplemented with inosine monophosphate

et al. 2011

Self Cite

View full text Add to dashboard Cite

Two feeding experiments were conducted to determine the effect of a 5 0 -inosine monophosphate (IMP)-supplemented casein peptide-based microdiet (MD) on the feeding activity, growth and survival of Pacific bluefin tuna (PBT) larvae. PBT larvae [total length: 14.0 mm (Exp. 1) or 13.3 mm (Exp. 2)] were fed a casein peptide-based MD supplemented with or without 3% IMP for 12 days. As a negative control, a limited number of yolk-sac larvae of spangled emperor Lethrinus nebulosus were fed only for the first 7 days and starved for the next 5 days. A sufficient amount of yolk-sac larvae of spangled emperor was fed in the Exp. 2. All fish in the control group had died by 9 or 10 days after the experiment. Extensive mortality was observed in fish fed the MD. The MD was only found in the stomach contents of PBT fed the MD ? IMP. Of the fish fed an IMP-supplemented diet, 50% survived for 12 days after the initiation of both feeding experiments. In addition, significantly higher growth than that observed during the first 7 days was seen in the PBT in the IMP treatment after 12 days of the feeding experiment. These results suggest that the supplementation of casein peptide-based MD with IMP could improve the feeding activity, survival and growth performance of PBT.

show abstract

Ontogenetic changes in RNA, DNA and protein contents of laboratory-reared Pacific bluefin tuna Thunnus�orientalis

Cited by 37 publications

References 19 publications

Tissue effect on RNA:DNA ratios of marine fish larvae

Tissue effect on RNA:DNA ratios of marine fish larvae

Ontogenetic changes in nucleic acid, protein contents, and growthof larval and juvenile Japanese flounder

Improvement in the feeding activity, early growth and survival of Pacific bluefin tuna Thunnus orientalis larvae fed a casein peptide-based microdiet supplemented with inosine monophosphate

Contact Info

Product

Resources

About