Ontogenetic approach to the assessment of plant resistance to prolonged stress using chlorophyll fluorescence induction method

Nesterenko, T. V.; Tikhomirov, A. A.; Shikhov, V. N.

doi:10.1007/s11099-006-0031-8

Cited by 19 publications

(17 citation statements)

References 58 publications

(85 reference statements)

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“…Since different quality radiation treatments could induce different responses of photosynthesis and Chl fluorescence (Sun et al 1998, Nishio 2000, Feild et al 2001, Frak et al 2002, Matsuda et al 2004, Nesterenko et al 2006, it is reasonable to speculate that measuring photosynthesis and Chl fluorescence with different quality radiation might cause different results too, which would further puzzle the comparison of these parameters between leaves of different color. However, in comparison of photosynthesis and Chl fluorescence between red and green leaves, little attention has been paid to the influences of different measuring radiations on these characteristics.…”

Section: Introductionmentioning

confidence: 99%

Co<sub>2</sub> assimilation and chlorophyll fluorescence in green versus red Berberis thunbergii leaves measured with different quality irradiation

Li¹,

Cheng²,

Peng³

et al. 2009

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Photosynthesis, photorespiration, and chlorophyll (Chl) fluorescence in green and red Berberis thunbergii leaves were studied with two different measuring radiations, red (RR) and "white" (WR). The photosynthetic and photorespiration rates responded differently to the different radiation qualities, which indicate that the carboxylase and oxygenase activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) were affected. Differences in photosynthetic rate between the two color leaves were less under RR than under WR. However, this reduced difference in photosynthetic rate was not correlated with the stomatal response to the measuring radiation qualities. Compared with the WR, the RR reduced the differences in dark-adapted minimum and maximum fluorescence, steady-state fluorescence, light-adapted maximum fluorescence, and actual photochemical efficiency (Φ PS2 ) of photosystem 2 (PS2), but enlarged the difference in non-photochemical quenching between the two color leaves. Differences in both maximum quantum yield of PS2 and ratio of Φ PS2 to quantum yield of CO 2 fixation between the two color leaves were similar under the two measuring radiations. To exclude disturbance of radiation attenuation caused by anthocyanins, it is better to use RR to compare the photosynthesis and Chl fluorescence in green versus red leaves.

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Section: Introductionmentioning

confidence: 99%

Co<sub>2</sub> assimilation and chlorophyll fluorescence in green versus red Berberis thunbergii leaves measured with different quality irradiation

Li¹,

Cheng²,

Peng³

et al. 2009

Photosynt.

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“…The F p /F t ratio is known to undergo consistent age dependent changes toward the steady state; the dura tion of stationary state coincides with period of leaf active functioning [7,19]. However, the F p /F t ratio does not provide precise assessment of the leaf age state at the stage of leaf maturity and beginning of senescence, because this ratio fluctuates in relation to metabolic and developmental changes of the whole plant [20].…”

Section: Chlorophyll Fluorescence As An Indicator Of Age Dependent Chmentioning

confidence: 99%

“…It is known that these parameters are affected by stress factors [3][4][5][6] and undergo changes during natural senescence of plant leaves [5,[7][8][9][10][11][12]. The age dependence of chlorophyll fluorescence parameters is a well established fact.…”

Section: Introductionmentioning

confidence: 99%

Chlorophyll fluorescence as an indicator of age-dependent changes in photosynthetic apparatus of wheat leaves

Nesterenko

Shikhov

Tikhomirov

2015

Russ J Plant Physiol

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Wheat (Triticum sativus L.) seedlings of various ages (2 to 16 day old plants) were used to study age dependent changes in the chlorophyll fluorescence induction (CFI) at various light intensities during flu orescence measurements. Plants were raised in a growth chamber using hydroponics with expanded clay, con trolled environmental conditions, and 690 µmol/(m 2 s) photon flux density (PFD) of photosynthetically active radiation (PAR). Parameters of CFI were determined under actinic PFD of 380, 580, 820, and 1340 µmol/(m 2 s) PAR. The fifth leaf from the stem base, exposed to uniform lighting, was sampled for mea surements. This leaf emerged at the plant age of 16 days. Based on fluorescence data, we calculated the max imal photochemical quantum yield of photosystem II (F v /F m ), the effective photochemical quantum yield of PSII (Yield), parameters of photochemical (qP) and non photochemical (qN and NPQ) quenching of chlo rophyll fluorescence, the F p /F t ratio, and the "vitality index" (fluorescence decrease ratio, R fd ). At moderate actinic PFD, applied commonly in PAM fluorometers (about 380 µmol/(m 2 s)), age dependent changes in NPQ, F p /F t , and R fd were observed. Analysis of CFI parameters in wheat leaves of different ages at PFD increasing from 380 to 820 µmol/(m 2 s) revealed that R fd , NPQ, and qN are the most sensitive markers of the leaf age among all parameters tested. These suitable indicators can be used for rapid assessment of the leaf age.

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mentioning

confidence: 99%

“…This is particularly important in the case of long-term exposure of the plants to unfavorable factors, as very often the only response of the photosynthetic apparatus of the leaves to such exposure is accelerated aging (Nesterenko et al 2003, Nesterenko et al 2006. Then, determination of the starting point of leaf senescence may be a way to evaluate resistance of the leaf photosynthetic apparatus to these factors (Nesterenko et al 2006). As the age heterogeneity of plant leaves usually exists, one should take into account the influence of the development stage of the leaf on the q N light curves, especially when this parameter is used to study the response of the leaf photosynthetic apparatus to stress.…”

mentioning

confidence: 99%

Effect of light intensity on the age dependence of nonphotochemical fluorescence quenching in wheat leaf

Nesterenko¹,

Shikhov²,

Tikhomirov³

2015

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The effects of actinic light (AL) intensity on the age dependence of nonphotochemical quenching of fluorescence (q N ) and effective quantum yield in PSII ( PSII ) were studied in continuously illuminated wheat leaves of the upper tier. Regular changes were revealed in both age dependence of q N at elevated AL intensities and light curves of q N . These changes are related to alterations in strategies of redistribution and use of absorbed light energy by the photosynthetic apparatus at different stages of wheat leaf development. Unlike Ф PSII , q N as a parameter was more sensitive to the differences in the leaf age at a certain range of light intensity. At the same time, the stability of q N at moderate light intensities may serve as an indication of leaf maturity.Additional key words: chlorophyll fluorescence induction; leaf age; photosystem II.--Nonphotochemical quenching of fluorescence (q N ) is commonly used to analyze stress tolerance in plants (Baker 2008, Ashraf and Harris 2013). It is well known that nonphotochemical quenching of chlorophyll fluorescence, q N , involves at least three molecular mechanisms: ΔpH-dependent quenching, which determines energy-dependent quenching of fluorescence (q E or the fast component, q Nf ); transition of the pigment system from state 1 to state 2 (q T or the medium component, q Nm ); and photoinactivation of PSII (q l or the slow component, q Ns ) (Baker, 2008). The contribution of each component to the total nonphotochemical quenching, q N , is determined by the actinic light intensity (Bukhov 2004), and their proportions and significance change during the leaf ontogeny (Hong et al. 1999, Müller et al. 2001, which may affect age variations in the q N as a whole. Indeed, under low and medium intensities of actinic light (AL), differences in the q N between mature and aged leaves are insignificant. These differences, however, become greater as AL intensity increases (Hong et al. 1999). Mechanisms determining nonphotochemical quenching of excited states of chlorophyll are mainly studied for PSII, as this photosystem is considered to be more sensitive to photoinhibition than PSI. Although it is well known that q N is age-and light-dependent, the age dependence of q N under medium intensities of AL and the effect of an increase in the AL intensity have not been sufficiently studied. No data have been reported on light curves of q N for leaves of different ages. The actinic light intensities reported in the literature varied between 200 and 1,500 μmol m -2 s -1 PPFD (Hong et al. 1999;Lichtenthaler et al. 2005; Wang and Chen 2011), and the available experimental data are not sufficient to evaluate the effect of the AL on the age dependence of the q N in the plant leaves. Thus, the purpose of this ---Abbreviations: CFI -chlorophyll fluorescence induction; F0 -minimal fluorescence yield of the dark-adapted state; F0' -minimal fluorescence yield of the light-adapted state; Fm -maximal fluorescence yield of the dark-adapted state; Fm' -maximal fluorescence yield of the light-ad...

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Ontogenetic approach to the assessment of plant resistance to prolonged stress using chlorophyll fluorescence induction method

Cited by 19 publications

References 58 publications

Co<sub>2</sub> assimilation and chlorophyll fluorescence in green versus red Berberis thunbergii leaves measured with different quality irradiation

Co<sub>2</sub> assimilation and chlorophyll fluorescence in green versus red Berberis thunbergii leaves measured with different quality irradiation

Chlorophyll fluorescence as an indicator of age-dependent changes in photosynthetic apparatus of wheat leaves

Effect of light intensity on the age dependence of nonphotochemical fluorescence quenching in wheat leaf

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