Ontogenesis of serotonin ‘receptors’ in different regions of rat brain

Узбеков, М. Г.; Murphy, Seán; Rose, Steven

doi:10.1016/0006-8993(79)90139-2

Cited by 56 publications

(10 citation statements)

References 11 publications

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“…Recent data (Uzbekov et al, 1979) indicate that there are high levels of 5-HT receptor binding early in development which then fall to adult levels. These findings are in contrast to receptor binding studies of other putative transmitter receptors (see Uzbekov et al, 1979), where binding sites are reported to be very low during the early stages of postnatal development.…”

Section: Discussionmentioning

confidence: 97%

Serotoninergic development in the postnatal rat brain

Hedner

Lundborg

1980

J. Neural Transmission

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Various characteristics of the developing serotoninergic system in the brain of rats aged 1 to 28 days were studied biochemically. The levels of the precursor amino acid tryptophan showed a maximal increase in the blood, brain and cerebrospinal fluid (CSF) during the 7th and 10th postnatal days. The development of tryptophan hydroxlyase activity measured in vivo by means of 5-hydroxytryptophan (5-HTP) accumulation after NSD 1015 was closely related to the 5-hydroxytryptamine (5-HT) levels at the various ages. 5-HTP accumulation and 5-HT levels increased most markedly after the second postnatal week. 5-Hydroxyindoleacetic acid (5-HIAA) levels were found to increase rapidly in the brain but somewhat more slowly in the CSF during the second week of postnatal development. Regional studies of 5-HTP accumulation after NSD 1015, 5-HT and 5-HIAA levels indicated a caudal to rostral way of maturation. The disappearance of 5-HT was measured after inhibition of tryptophan hydroxylase with H 22/54. The half-life generally decreased in the various brain parts with advancing age, and in the younger animals the shortest half-life was found in the most caudal brain parts. At 28 days of age the half-life was similar in all brain parts studied. These results indicate the existence of an adult like nerve impulse flow in the 5-HT neurons in the brain stem region of the newborn rats. The results from this investigation clearly indicate that th maturation of the different biochemical parameters of the 5-HT pathways develop in a caudal to rostral direction. The study also supports the view that tryptophan hydroxylase may be the limiting step in the development of the serotoninergic system.

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Section: Discussionmentioning

confidence: 97%

Serotoninergic development in the postnatal rat brain

Hedner

Lundborg

1980

J. Neural Transmission

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“…5-HT receptor assay was done using [ 3 H] 5-hydroxytryptamine binding in crude synaptic membrane preparations of hippocampus by the modified method of [18]. Crude membrane preparation was suspended in 50 mM Tris-HCl buffer, pH 8.5, containing 1.0 μM paragyline.…”

Section: Methodsmentioning

confidence: 99%

Alterations in hippocampal serotonergic and INSR function in streptozotocin induced diabetic rats exposed to stress: neuroprotective role of pyridoxine and Aegle marmelose

et al. 2010

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Diabetes and stress stimulate hippocampal 5-HT synthesis, metabolism and release. The present study was carried out to find the effects of insulin, Aegle marmelose alone and in combination with pyridoxine on the hippocampal 5-HT, 5-HT2A receptor subtype, gene expression studies on 5-HT2A, 5-HTT, INSR, immunohistochemical studies and elevated plus maze in streptozotocin induced diabetic rats. 5-HT content showed a significant decrease (p < 0.001) and a significant increase (p < 0.001) in 5-HIAA in hippocampus of diabetic rats compared to control. 5-HT receptor binding parameters Bmax and Kd showed a significant decrease (p < 0.001) whereas 5-HT2A receptor binding parameters Bmax showed a significant decrease (p < 0.001) with a significant increase (p < 0.05) in Kd in hippocampus of diabetic rats compared to control. Gene expression studies of 5-HT2A, 5-HTT and INSR in hippocampus showed a significant down regulation (p < 0.001) in diabetic rats compared to control. Pyridoxine treated in combination with insulin and A. marmelose to diabetic rats reversed the 5-HT content, Bmax , Kd of 5-HT, 5-HT2A and gene expression of 5-HT2A, 5-HTT and INSR in hippocampus to near control. The gene expression of 5-HT2A and 5-HTT were confirmed by immunohistochemical studies. Behavioural studies using elevated plus maze showed that serotonin through its transporter significantly increased (p < 0.001) anxiety-related traits in diabetic rats which were corrected by combination therapy. Our results suggest that pyridoxine treated in combination with insulin and A. marmelose has a role in the regulation of insulin synthesis and release, normalising diabetic related stress and anxiety through hippocampal serotonergic function. This has clinical significance in the management of diabetes.

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“…Serotonin receptors have been divided into several different classes and subtypes based on their relative binding affinities for 5-HT and selective ligands, and their cloned sequences. Recently, the terminology describing these subtypes has been revised [see Hoyer et al, 19941. Early studies using receptor binding with 3H-5-HT as ligand described the postnatal ontogeny of 5-HT receptors in the rat brain [Uzbekov et al, 1979…”

Section: Development Of Serotonin Receptorsmentioning

confidence: 99%

Ontogeny of neurotransmitter systems: Substrates for developmental disabilities?

Lauder

1995

Ment. Retard. Dev. Disabil. Res. Rev.

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This papaer provides an overview of the developmental characteristics of several major CNS neurotransmitter systems, specifically those involving serotonin, catecholamines, and GABA. These were chosen because of their putative importance in various aspects of brain development or plasticity, and the existence of clinically relevant sequelae linking these systems to developmental disorders and disabilities. Patterns of development of these neurotransmitter systems and their putative roles as developmental signals are discussed, as is their possible relevance to developmental disabilities such as autism, attention‐deficit/hyperactivity disorders, self‐injurious behaviour, and epilepsy. © 1995 Wiley‐Liss, Inc.

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Ontogenesis of serotonin ‘receptors’ in different regions of rat brain

Cited by 56 publications

References 11 publications

Serotoninergic development in the postnatal rat brain

Serotoninergic development in the postnatal rat brain

Alterations in hippocampal serotonergic and INSR function in streptozotocin induced diabetic rats exposed to stress: neuroprotective role of pyridoxine and Aegle marmelose

Ontogeny of neurotransmitter systems: Substrates for developmental disabilities?

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