Onset and time course of apoptosis in the developing zebrafish retina

Biehlmaier, Oliver; Köhler, Konrad

doi:10.1007/s004410100447

Cited by 91 publications

(92 citation statements)

References 32 publications

(47 reference statements)

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“…During these imaging experiments, only two cells underwent apoptosis, as marked by nuclear condensation followed by DNA fragmentation (data not shown). This low rate of apoptosis (1.8%) within the zebrafish retinal neuroepithelium is consistent with previous reports on fixed tissue (Biehlmaier et al, 2001). The paucity of cell death also confirmed that our imaging approach did not introduce phototoxicity.…”

Section: Diversity In Retinal Progenitor Interkinetic Nuclear Migrationsupporting

confidence: 92%

Interkinetic Nuclear Migration and the Selection of Neurogenic Cell Divisions during Vertebrate Retinogenesis

Baye¹,

Link²

2007

J. Neurosci.

164

193

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During retinal development, neuroepithelial progenitor cells divide in either a symmetric proliferative mode, in which both daughter cells remain mitotic, or in a neurogenic mode, in which at least one daughter cell exits the cell cycle and differentiates as a neuron. Although the cellular mechanisms of neurogenesis remain unknown, heterogeneity in cell behaviors has been postulated to influence this cell fate. In this study, we analyze interkinetic nuclear migration, the apical-basal movement of nuclei in phase with the cell cycle, and the relationship of this cell behavior to neurogenesis. Using time-lapse imaging in zebrafish, we show that various parameters of interkinetic nuclear migration are significantly heterogeneous among retinal neuroepithelial cells. We provide direct evidence that neurogenic progenitors have greater basal nuclei migrations during the last cell cycle preceding a terminal mitosis. In addition, we show that atypical protein kinase C (aPKC)-mediated cell polarity is essential for the relationship between nuclear position and neurogenesis. Loss of aPKC also resulted in increased proliferative cell divisions and reduced retinal neurogenesis. Our data support a novel model for neurogenesis, in which interkinetic nuclear migration differentially positions nuclei in neuroepithelial cells and therefore influences selection of progenitors for cell cycle exit based on apical-basal polarized signals.

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Section: Diversity In Retinal Progenitor Interkinetic Nuclear Migrationsupporting

confidence: 92%

Interkinetic Nuclear Migration and the Selection of Neurogenic Cell Divisions during Vertebrate Retinogenesis

Baye¹,

Link²

2007

J. Neurosci.

164

193

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“…The numbers of TUNEL-positive cells in control retinas were very low at 36 hpf, 48 hpf, and 72 hpf (Figs. 3E, 3G, 3I, 3M), and on average, slightly lower than those reported by others (Biehlmaier et al, 2001;Cole & Ross, 2001). The numbers of TUNEL-positive cells in retinas of ethanoltreated embryos were only slightly greater (Fig.…”

Section: Ethanol Treatment Results In Modest Effects On Retinal Histomentioning

confidence: 51%

Mechanisms for persistent microphthalmia following ethanol exposure during retinal neurogenesis in zebrafish embryos

2007

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The exposure of the developing human embryo to ethanol results in a spectrum of disorders involving multiple organ systems, including the visual system. One common phenotype seen in humans exposed to ethanol in utero is microphthalmia. The objective of this study was to describe the effects of ethanol during retinal neurogenesis in a model organism, the zebrafish, and to pursue the potential mechanisms by which ethanol causes microphthalmia. Zebrafish embryos were exposed to 1% or 1.5% ethanol from 24 to 48 h after fertilization, a period during which the retinal neuroepithelium undergoes rapid proliferation and differentiation to form a laminated structure composed of different retinal cell types. Ethanol exposure resulted in significantly reduced eye size immediately following the treatment, and this microphthalmia persisted through larval development. This reduced eye size could not entirely be accounted for by the accompanying general delay in embryonic development. Retinal cell death was only slightly higher in ethanol-exposed embryos, although cell death in the lens was extensive in some of these embryos, and lenses were significantly reduced in size as compared to those of control embryos. The initiation of retinal neurogenesis was not affected, but the subsequent waves of cell differentiation were markedly reduced. Even cells that were likely generated after ethanol exposure-rod and cone photoreceptors and Müller glia-were delayed in their expression of cell-specific markers by at least 24 h. We conclude that ethanol exposure over the time of retinal neurogenesis resulted in persistent microphthalmia due to a combination of an overall developmental delay, lens abnormalities, and reduced retinal cell differentiation.

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“…In the zebrafish, a series of cell-specific waves of apoptotic cell death has been documented to take place subsequent to the waves of cell birth (Biehlmaier et al, 2001). These waves of cell death follow the same sequence, with an initial wave of cell death in the ganglion cell layer followed by cell death in the inner and then outer nuclear layer.…”

Section: A Spatiotemporal Patternsmentioning

confidence: 99%

Neurogenesis in the Fish Retina

Stenkamp

2007

International Review of Cytology

122

136

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The retinas of teleost fish have long been of interest to developmental neurobiologists for their persistent plasticity during growth, life history changes, and response to injury. Because the vertebrate retina is a highly conserved tissue, the study of persistent plasticity in teleosts has provided insights into mechanisms for postembryonic retinal neurogenesis in mammals. In addition, in the past 10 years there has been an explosion in the use of teleost fish-zebrafish (Danio rerio) in particular-to understand the mechanisms of embryonic retinal neurogenesis in a model vertebrate with genetic resources. This review summarizes the key features of teleost retinal neurogenesis that make it a productive and interesting experimental system, and focuses on the contributions to our knowledge of retinal neurogenesis that uniquely required or significantly benefited from the use of a fish model system.

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Onset and time course of apoptosis in the developing zebrafish retina

Cited by 91 publications

References 32 publications

Interkinetic Nuclear Migration and the Selection of Neurogenic Cell Divisions during Vertebrate Retinogenesis

Interkinetic Nuclear Migration and the Selection of Neurogenic Cell Divisions during Vertebrate Retinogenesis

Mechanisms for persistent microphthalmia following ethanol exposure during retinal neurogenesis in zebrafish embryos

Neurogenesis in the Fish Retina

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