Only the soluble form of the scavenger receptor CD163 acts inhibitory on phorbol ester‐activated T‐lymphocytes, whereas membrane‐bound protein has no effect

Frings, Werner; Dreier, Jens; Sorg, Clemens

doi:10.1016/s0014-5793(02)03142-3

Cited by 92 publications

(71 citation statements)

References 22 publications

(23 reference statements)

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“…They include IL-1 ␣ , IL-1 receptor antagonist, IL-6, TNF ␣ , pulmonary and activation-regulated chemokine, and soluble IL-2 receptor ( 229-232 ). Such mediators clearly could play a role in disease progression (233)(234)(235). In ASM knockout mice, the majority of genes with elevated expression in lung and brain were those involved in the immune/ infl ammatory response ( 236 ).…”

Section: Infl Ammationmentioning

confidence: 99%

Multi-system disorders of glycosphingolipid and ganglioside metabolism

Xu¹,

Barnes²,

Sun³

et al. 2010

Journal of Lipid Research

142

121

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important to a variety of cellular functions. GSLs and gangliosides are synthesized at the endoplasmic reticulum (ER) and are remodeled during transit from cis to trans Golgi by a series of glycosyl-and sialyl-transferases. These are then transported to the intracellular compartments and the plasma membrane where they become enriched in microdomains and membrane bilayers. During plasma membrane turnover, GSLs and gangliosides can be internalized and partially or completely degraded in the endosomal/lysosomal system to sphingosine and free fatty acids that are then transported or fl ipped across late endosomal and lysosomal membranes for recycling or for use as signaling molecules ( 2, 3 ). GSL metabolic pathwaysGSL biosynthesis begins with condensation of serine and palmitoyl-CoA catalyzed by serine-palmitoyltransferase (SPT) on the cytoplasmic face of the ER, leading to de novo biosynthesis of ceramide, the core of GSLs (

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Section: Infl Ammationmentioning

confidence: 99%

Multi-system disorders of glycosphingolipid and ganglioside metabolism

Xu¹,

Barnes²,

Sun³

et al. 2010

Journal of Lipid Research

142

121

View full text Add to dashboard Cite

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“…Also some reports show that CD163 soluble molecule may inhibit human T lymphocytes activation and proliferation in vitro (12,22), thereby facilitate suppression of the inflammatory response (5,22). Frigs and co-workers indicated that the soluble form of CD163 might have a direct suppressive action on CD4+ T helper cells (5).…”

Section: Functions Of Cd163 Scavenger Receptormentioning

confidence: 99%

Scavenger Receptor CD163 and Its Biological Functions

Onofre

Koláčková

Jankovicova

et al. 2009

Acta Med. (Hradec Kralove, Czech Repub.)

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Summary: CD163 is a member of scavenger receptor super family class B of the first subgroup. It is mapped to the region p13 on chromosome 12. Five different isoforms of CD163 have been described, which differ in the structure of their cytoplasmic domains and putative phosporylation sites. This scavenger receptor is selectively expressed on cells of monocytes and macrophages lineage exclusively. CD163 immunological function is essentially homeostatic. It also has other functions because participates in adhesion to endothelial cells, in tolerance induction and tissues regeneration. Other very important function of CD163 is the clearance of hemoglobin in its cell-free form and participation in anti-inflammation in its soluble form, exhibiting cytokine-like functions. We review the biological functions of CD163 which have been discovered until now. It seems apparent from this review that CD163 scavenger receptor can be used as biomarker in different diseases and as a valuable diagnostic parameter for prognosis of many diseases especially inflammatory disorders and sepsis.

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“…Elevated plasma levels of sCD163 could be therefore a good indicator of an ongoing inflammatory or activation process of tissue macrophages. Besides, sCD163 appears to have immunosuppresive effects, inhibiting the activation and proliferation of T lymphocytes at concentrations not higher than physiological levels (Hogger and Sorg, 2001;Frings et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Cloning and expression of porcine CD163: its use for characterization of monoclonal antibodies to porcine CD163 and development of an ELISA to measure soluble CD163 in biological fluids

Pérez¹,

Ezquerra²,

Ortuño³

et al. 2008

Span. j. agric. res.

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CD163 is a member of the family of proteins with scavenger receptor cysteine-rich domains, whose expression is restricted to monocytes and macrophages. It functions as a receptor for haemoglobin/haptoglobin complexes and it has also been implicated in the regulation of inflammatory processes. Treatment of monocytes/macrophages with phorbol esters, LPS or cross-linking of Fc gamma receptors, causes shedding of CD163 from cell surface by a protease-dependent mechanism. The level of soluble CD163 (sCD163) in serum and other body fluids has been considered a useful marker of macrophage activation. In addition, porcine CD163 has been shown to play a role in the infection of monocytes/macrophages by the African swine fever virus. The porcine CD163 cDNA has been cloned, and expressed in transfected CHO cells. Clones of CHO cells stably expressing porcine CD163 have been used for the characterization of three new mAbs against porcine CD163. Using two of these mAbs, that bind to different epitopes on CD163 molecule, a sandwich enzyme-linked immunoassay (ELISA) has been developed to measure levels of sCD163 in porcine sera and biological fluids. The assay was calibrated using lysates of CD163 transfectants, showing a sensitivity of 10 5 cells mL -1 , that allowed to detect sCD163 in sera and in culture supernatants of activated alveolar macrophages. This assay can be a useful method of monitoring the degree of activation of macrophages in a variety of inflammatory and infectious diseases of swine.Additional key words: macrophage, SRCR family, swine. ResumenClonación y expresión de CD163 porcino: aplicación en la caracterización de anticuerpos monoclonales frente a CD163 porcino, y desarrollo de un ELISA para cuantificar CD163 soluble en fluidos biológicos CD163 es un miembro de la familia de proteínas con dominios de receptores «scavenger» ricos en cisteína, con una expresión restringida a células del linaje monocítico. Se ha demostrado su función como receptor de complejos hemoglobina/haptoglobina, y se le ha implicado en la regulación de procesos inflamatorios. El tratamiento de monocitos/macrófagos con ésteres de forbol, LPS, o mediante entrecruzamiento de receptores Fc gamma, provoca la liberación de CD163 de la superficie celular por un mecanismo dependiente de proteasas. La cantidad de CD163 soluble (sCD163) en suero y otros fluidos corporales se considera un indicador de la activación de los macrófagos. Recientemente, nuestro grupo demostró que CD163 está implicado en la infección de monocitos/macrófagos porcinos por el virus de la peste porcina africana. En este trabajo se describe la clonación del cDNA de CD163 porcino, y su expresión en células CHO transfectadas. Se obtuvieron clones de células CHO transfectadas que expresan de forma estable la molécula CD163 porcina, y se utilizaron para caracterizar tres nuevos anticuerpos monoclonales que reconocen específicamente CD163 porcino. El uso de dos de estos anticuerpos, que reconocen epítopos diferentes en CD163 porcino, ha permitido desarrollar un ensayo i...

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Only the soluble form of the scavenger receptor CD163 acts inhibitory on phorbol ester‐activated T‐lymphocytes, whereas membrane‐bound protein has no effect

Cited by 92 publications

References 22 publications

Multi-system disorders of glycosphingolipid and ganglioside metabolism

Multi-system disorders of glycosphingolipid and ganglioside metabolism

Scavenger Receptor CD163 and Its Biological Functions

Cloning and expression of porcine CD163: its use for characterization of monoclonal antibodies to porcine CD163 and development of an ELISA to measure soluble CD163 in biological fluids

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