Online preconcentration of thyrotropin-releasing hormone (TRH) by SDS-modified reversed phase column for microbore and capillary high-performance liquid chromatography (HPLC)

Rong, Meng; Xia, Wanlin; Sandberg, Mats; Stephens, Robert L.; Weber, Stephen G.

doi:10.1016/j.chroma.2004.12.032

Cited by 6 publications

(3 citation statements)

References 40 publications

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“…The capillary HPLC system was described before[35, 36], so the process is briefly summarized as follows.…”

Section: Methodsmentioning

confidence: 99%

Carbon fiber/epoxy composite ring–disk electrode: Fabrication, characterization and application to electrochemical detection in capillary high performance liquid chromatography

Weber

2009

Journal of Electroanalytical Chemistry

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Carbon fiber/epoxy composite materials, which are manufactured using the pultrusion process, are commercially available in various shapes and sizes at very low cost. Here we demonstrate the application of such a material as an electrochemical detector in a flow system. Cyclic voltammetry shows that the material's electrochemical behavior resembles that of glassy carbon. Using tube and rod composites, we successfully fabricated a ring-disk electrode with a 20 μm gap between the ring and the disk. The narrow gap is favorable for mass transfer in the generator-collector experiment. This composite ring-disk electrode is assembled in a thin-layer radial-flow cell and used as an electrochemical detector. The disk electrode, placed directly opposite to the flow inlet, is operated as a generator electrode with the ring electrode being a collector. The high collection efficiency on the ring electrode (0.8 for a chemically reversible species) enhances the detection selectivity.

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“…The capillary HPLC system was described before[35, 36], so the process is briefly summarized as follows.…”

Section: Methodsmentioning

confidence: 99%

Carbon fiber/epoxy composite ring–disk electrode: Fabrication, characterization and application to electrochemical detection in capillary high performance liquid chromatography

Weber

2009

Journal of Electroanalytical Chemistry

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“…Therefore, analytical approaches combining separation with detection systems are preferred. [9][10][11][12][13][14][15][16] Recently, Meng et al realized online preconcentration and electrochemical detection of TRH on SDS-modified precolumns with a detection limit of 3 nM in 24 μL. 11 The LC-MS method was also employed for TRH quantification with a satisfactory detection limit (1 fmol).…”

Section: Introductionmentioning

confidence: 99%

“…[9][10][11][12][13][14][15][16] Recently, Meng et al realized online preconcentration and electrochemical detection of TRH on SDS-modified precolumns with a detection limit of 3 nM in 24 μL. 11 The LC-MS method was also employed for TRH quantification with a satisfactory detection limit (1 fmol). 16 UV absorbance and electrochemical detection have a drawback of low sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive detection of thyrotropin-releasing hormone based on azo coupling and surface-enhanced resonance Raman spectroscopy

Sui

Wang

Zhang

et al. 2016

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Surface-enhanced resonance Raman scattering (SERRS) has been used to establish a rapid and quantitative assay based on the diazotization coupling reaction for thyrotropin-releasing hormone (TRH). Ultrahigh sensitivity of this approach originates from two factors: changing TRH to an azo compound and the SERRS effect with the addition of silver nanoparticles (AgNPs) at 532 nm excitation wavelength. The lowest detectable concentration of TRH was found to be as low as 1 pg mL(-1), which is 10-fold lower than the lowest normal reference value in human serum reported in previous literature. The quantitative measurements in human serum based on this method were conducted, and the results showed its feasibility for detection in complex biological samples. In comparison with conventional TRH identification and quantification methodologies, radioimmunoassay (RIA) and subsequent various hyphenated techniques, the main advantages of this study are simplicity, rapidness (2 minutes), time effectiveness, no additional steps required to further characterize the immunogenic material, highest sensitivity (57.1 fg), high selectivity, practicality and reliability. Thus, this work puts forward a research tool that may be applied to the determination of TRH in practical assays.

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Green Chromatography

2012

Handbook of Green Analytical Chemistry

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Greening the university laboratories 3.2Green laboratory experiments 3.2.1Green methods for sample pretreatment 3.2.2 Green separation using liquid-liquid, solid-phase and solventiess extractions 3.2.3Green alternatives for chemical reactions 3.2.4Green spectroscopy 3.3The place of Green Analytical Chemistry in the future of our laboratories References vi Contents 4 Publishing in Green Analytical Chemistry 55 Salvador G'arrigues and Miguel de la Guardia 4.1 A bibliometric study of the literature in Green Analytical Chemistry 56 4.2 Milestones of the literature on Green Analytical Chemistry 57 4.

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Online preconcentration of thyrotropin-releasing hormone (TRH) by SDS-modified reversed phase column for microbore and capillary high-performance liquid chromatography (HPLC)

Cited by 6 publications

References 40 publications

Carbon fiber/epoxy composite ring–disk electrode: Fabrication, characterization and application to electrochemical detection in capillary high performance liquid chromatography

Carbon fiber/epoxy composite ring–disk electrode: Fabrication, characterization and application to electrochemical detection in capillary high performance liquid chromatography

Ultrasensitive detection of thyrotropin-releasing hormone based on azo coupling and surface-enhanced resonance Raman spectroscopy

Green Chromatography

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