Online monitoring of stable carbon isotopes of methane in anaerobic digestion as a new tool for early warning of process instability

Polag, Daniela; May, T.; Müller, Liane; König, Helmut; Jacobi, Fabian; Laukenmann, Stephan; Keppler, Frank

doi:10.1016/j.biortech.2015.08.058

Cited by 40 publications

(32 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The shift in predominant methanogenic pathways is reflected in the isotope composition of the produced methane; therefore, it can be used as a process‐monitoring tool and has potential for AD optimization . The strong isotope effect associated with the partial or complete ammonia inhibition is a promising target regarding the applicability of the isotope concept in process monitoring of large‐scale biogas plants using nitrogen‐rich substrates .…”

Section: Introductionmentioning

confidence: 99%

Ammonium Chloride vs Urea‐Induced Ammonia Inhibition of the Biogas Process Assessed by Stable Isotope Analysis

Jiang

Liebetrau

et al. 2018

Chem Eng & Technol

View full text Add to dashboard Cite

Anaerobic digestion of poultry manure poses many challenges due to ammonia inhibition by nitrogen‐containing compounds. Here, the effect of NH4Cl or urea‐induced inhibition was investigated in bioreactors fed with maize silage or cellulose and compared to previous experiments with chicken manure as ammonia source. The hydrogen and carbon stable isotope compositions of biogas samples were evaluated to assess the shifts in predominant methanogenic pathways during increasing ammonia inhibition. Due to the higher free ammonia concentrations, stronger inhibition was observed in the urea added reactors compared to the NH4Cl treated ones. Collectively, the results suggested that ammonium salts are not really appropriate to investigate ammonia inhibition during anaerobic digestion of manure.

show abstract

Section: Introductionmentioning

confidence: 99%

Ammonium Chloride vs Urea‐Induced Ammonia Inhibition of the Biogas Process Assessed by Stable Isotope Analysis

Jiang

Liebetrau

et al. 2018

Chem Eng & Technol

View full text Add to dashboard Cite

show abstract

“…The correlation between the microbial communities and reactor parameters was established by multivariate data analysis. In addition, the dominant methanogenic pathway was determined by the stable isotope fingerprinting of the produced biogas, following approaches applied to various anaerobic systems [ 20 ] and biogas reactors with several substrates [ 7 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Lessons learned from the microbial ecology resulting from different inoculation strategies for biogas production from waste products of the bioethanol/sugar industry

Leite

Janke

Harms

et al. 2016

Biotechnol Biofuels

View full text Add to dashboard Cite

BackgroundDuring strategic planning of a biogas plant, the local availability of resources for start-up and operation should be taken into consideration for a cost-efficient process. Because most bioethanol/sugar industries in Brazil are located in remote areas, the use of fresh cattle manure from local farms could be a solution for the inoculation of the biogas process. This study investigated the diversity and dynamics of bacterial and archaeal communities and the performance of biogas reactors inoculated with manure and a mixed inoculum from different biogas reactors as for a controlled start-up until steady state.ResultsLaboratory-scale biogas reactors were fed semi-continuously with sugarcane filter cake alone (mono-digestion) or together with bagasse (co-digestion). At the initial start-up, the reactors inoculated with the mixed inoculum displayed a less diverse taxonomic composition, but with higher presence of significant abundances compared to reactors inoculated with manure. However, in the final steady state, the communities of the differently inoculated reactors were very similarly characterized by predominance of the methanogenic genera Methanosarcina and Methanobacterium, the bacterial families Bacteroidaceae, Prevotellaceae and Porphyromonadaceae (phylum Bacteroidetes) and Synergistaceae (phylum Synergistetes). In the mono-digestion reactors, the methanogenic communities varied greater than in the co-digestion reactors independently of the inoculation strategy.ConclusionThe microbial communities involved in the biogas production from waste products of the Brazilian bioethanol/sugar industry were relatively similar and stable at the reactor’s steady phase independently of the inoculum source (manure or mixed inoculum). Therefore, the locally available manure can be used as inoculum for start-up of the biogas process, since it also contains the microbial resources needed. The strong fluctuation of methanogenic communities in mono-digestion reactors indicates higher risk of process instability than in co-digestion reactors.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0548-4) contains supplementary material, which is available to authorized users.

show abstract

“…In biogas plants, microbial organisms produce CH 4 under anaerobic conditions. The isotopic signature of CH 4 in biogas can vary widely due to the substrate, the microbial producers of CH 4 and kinetic values like temperature and frequency of feeding (Polag et al, 2015; personal communication with D. Polag, 2017).…”

Section: Biogas Plantmentioning

confidence: 99%

Characterisation of δ<sup>13</sup>CH<sub>4</sub> source signatures from methane sources in Germany using mobile measurements

Hoheisel

Yeman

Dinger

et al. 2018

Preprint

View full text Add to dashboard Cite

Abstract. The carbon isotopic signature (δ13CH4) of several methane sources in Germany (around Heidelberg and in North Rhine-Westphalia) were characterised. Therefore, mobile measurements of the plume of CH4 sources are carried out using a cavity ring-down spectrometer (CRDS). To achieve precise results a CRDS analyser, which measures methane (CH4), carbon dioxide (CO2) and their 13C to 12C ratios, was characterised especially with regard to cross sensitivities of the gas matrix. The two most important gases which affect the measurements are water vapour (H2O) and ethane (C2H6). To avoid the cross sensitivity with H2O, the air is dried with a nafion dryer during mobile measurements. C2H6 is abundant in natural gas and thus in methane plumes or samples originating from natural gas. A C2H6 correction and calibration are essential to obtain accurate δ13CH4 results, which can deviate up to 3 ‰ depending on whether an ethane correction is applied. The isotopic signature is determined with the Miller-Tans approach and the York fitting method. During 21 field campaigns the mean δ13CH4 values of three dairy farms (−63.9 ± 0.9 ‰), a biogas plant (−62.4 ± 1.2 ‰), a landfill (−58.7 ± 3.3 ‰), a wastewater treatment plant (−52.5 ± 1.4 ‰), an active deep coal mine (−56.0 ± 2.3 ‰) and two natural gas storage and gas compressor stations (−46.1 ± 0.8 ‰) were recorded. In addition, between December 2016 and June 2018 gas samples from the Heidelberg natural gas distribution network were measured. Contrary to former measurements between 1991 and 1996 (Levin et al., 1999) no strong seasonal cycle is shown. The mean δ13CH4 value of this study is −43.1 ± 0.8 ‰ which is 2.8 ‰ more depleted than in former years.

show abstract

Online monitoring of stable carbon isotopes of methane in anaerobic digestion as a new tool for early warning of process instability

Cited by 40 publications

References 35 publications

Ammonium Chloride vs Urea‐Induced Ammonia Inhibition of the Biogas Process Assessed by Stable Isotope Analysis

Ammonium Chloride vs Urea‐Induced Ammonia Inhibition of the Biogas Process Assessed by Stable Isotope Analysis

Lessons learned from the microbial ecology resulting from different inoculation strategies for biogas production from waste products of the bioethanol/sugar industry

Characterisation of δ<sup>13</sup>CH<sub>4</sub> source signatures from methane sources in Germany using mobile measurements

Contact Info

Product

Resources

About

Online monitoring of stable carbon isotopes of methane in anaerobic digestion as a new tool for early warning of process instability

Cited by 40 publications

References 35 publications

Ammonium Chloride vs Urea‐Induced Ammonia Inhibition of the Biogas Process Assessed by Stable Isotope Analysis

Ammonium Chloride vs Urea‐Induced Ammonia Inhibition of the Biogas Process Assessed by Stable Isotope Analysis

Lessons learned from the microbial ecology resulting from different inoculation strategies for biogas production from waste products of the bioethanol/sugar industry

Characterisation of δ&lt;sup&gt;13&lt;/sup&gt;CH&lt;sub&gt;4&lt;/sub&gt; source signatures from methane sources in Germany using mobile measurements

Contact Info

Product

Resources

About

Characterisation of δ<sup>13</sup>CH<sub>4</sub> source signatures from methane sources in Germany using mobile measurements