Online monitoring of P(3HB) produced from used cooking oil with near-infrared spectroscopy

Cruz, M.E.M.; Sarraguça, Mafalda C.; Freitas, Filomena; Lopes, João A.; Reis, Maria A.M.

doi:10.1016/j.jbiotec.2014.11.022

Cited by 45 publications

(29 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results obtained in experiment A, wherein the exponential feeding strategy was tested, are presented in Figure 1 and Table 1. During the initial batch phase of the experiment, the culture grew with a maximum specific cell growth rate of 0.14 ± 0.02 h −1 , which is similar to the values obtained in previous studies for cultivation of C. necator under batch mode, 0.12-0.14 h −1 [23,24].…”

Section: Resultssupporting

confidence: 87%

See 1 more Smart Citation

A Process Engineering Approach to Improve Production of P(3HB) byCupriavidus necatorfrom Used Cooking Oil

Cruz

Gouveia

Dionı́sio

et al. 2019

International Journal of Polymer Science

Self Cite

View full text Add to dashboard Cite

Different feeding strategies, namely, exponential feeding and DO-stat mode, were implemented for the production of poly(3-hydroxybutyrate), P(3HB), by Cupriavidus necator DSM 428 with used cooking oil (UCO) as the sole carbon source. With the exponential feeding strategy, a cell dry mass of 21.3 ± 0.9 g L −1 was obtained, with a polymer content of 84.0 ± 4.5 wt.%, giving an overall volumetric productivity of 4.5 ± 0.2 g L −1 day −1 . However, the highest P(3HB) volumetric productivity, 12.6 ± 0.8 g L −1 day −1 , was obtained when the DO-stat mode was implemented together with the use of ammonium hydroxide for pH control, which served as an additional nitrogen source and allowed to reach higher cell dry mass (7.8 ± 0.6 g L −1 ). The P(3HB) obtained in all experiments had a high molecular mass, ranging from 0.6 × 10 5 to 2.6 × 10 5 g mol −1 , with low polydispersity indexes of 1.2-1.6. Melting and glass transition temperatures were also similar for the polymer produced with both cultivation strategy, 174°C and 3.0-4.0°C, respectively. The polymer exhibited a crystallinity ranging from 52 to 65%. The DO-stat strategy to feed oil containing substrates as the sole carbon sources was reported for the first time in this study, and the preliminary results obtained show that it is a promising strategy to improve P(3HB) production. Nevertheless, the process requires further optimization in order to make it economically viable.

show abstract

Section: Resultssupporting

confidence: 87%

“…In the previous work, UCO was demonstrated to be a suitable substrate for the cultivation of C. necator DSM 428 for production of P(3HB) [23,24]. However, process optimization conditions were not explored.…”

Section: Introductionmentioning

confidence: 99%

A Process Engineering Approach to Improve Production of P(3HB) byCupriavidus necatorfrom Used Cooking Oil

Cruz

Gouveia

Dionı́sio

et al. 2019

International Journal of Polymer Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…These tools are supposed to provide nondestructive measurements at‐line, on‐line, or in‐line and aim to provide information about the bioprocess . One technique among a variety of technologies that can be used to gather real‐time information about the cultivation processes is 2D fluorescence spectroscopy (2DFS) . A fluorescence probe is used to record excitation/emission scans of the culture broth in the course of the cultivation.…”

Section: Introductionmentioning

confidence: 99%

“…1,3 One technique among a variety of technologies that can be used to gather real-time information about the cultivation processes is 2D fluorescence spectroscopy (2DFS). [4][5][6][7][8] A fluorescence probe is used to record excitation/emission scans of the culture broth in the course of the cultivation. 2DFS is highly sensitive toward changes in the culture and works well in water-containing fluids.…”

Section: Introductionmentioning

confidence: 99%

Exploring the capabilities of fluorometric online monitoring on chinese hamster ovary cell cultivations producing a monoclonal antibody

Schwab

Amann

Schmid

et al. 2016

Biotechnology Progress

View full text Add to dashboard Cite

Online monitoring of Chinese hamster ovary fed-batch cell cultures via two-dimensional fluorescence spectroscopy (2DFS) was evaluated in this work. Particular attention was directed toward different process strategies regarding the use of nutrient-rich feed media and temperature shifts. These intentionally performed process manipulations broadened the variances in the obtained fluorescence spectra and this was suspected to hamper the generation of reliable soft sensors. Principal component analysis of the obtained fluorescence data showed that temperature shift and feeding strategy had a considerable impact on the fluorescence signals. Partial least square regression models were calculated for the prediction of glucose, lactate, monoclonal antibody (mAb), and viable cell concentrations (VCC). It was aimed to integrate all 2DFS datasets in the respective calibration models regardless of the process-strategy-dependent diversity. Contrary to the expectations, it was feasible to calibrate soft sensors for the online prediction of glucose (7 latent variables (LVs), Rcal2 = 0.97, rout mean squared error of prediction (RMSEP) = 1.1 g L ), lactate (5 LV; Rcal2 = 0.96; RMSEP = 0.5 g L ) and mAb concentrations (4 LV; Rcal2 = 0.99; RMSEP = 11.4 mg L ). Feeding and temperature shifts had the highest impact on the VCC model (3 LV; Rcal2 = 0.94; RMSEP 3.8 × 10 mL ), nevertheless the prediction of VCC from the fed-batch 2DFS data was feasible. The results strongly indicate that variances in the datasets due to the process strategy can be tolerated to some extent by the respective soft sensors. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1592-1600, 2016.

show abstract

“…The identification of specific contiguous wavelength 223 windows for coffee acidity determination was focused in order to highlight the most important 224 chemical functional groups involved in the model prediction. This strategy for wavelength 225 selection was previously described in Cruz et al (2015). 226 PLS models performance was primarily evaluated with the root mean square error of 227 cross validation (RMSECV).…”

mentioning

confidence: 99%

Exploiting near infrared spectroscopy as an analytical tool for on-line monitoring of acidity during coffee roasting

et al. 2016

Self Cite

View full text Add to dashboard Cite

Online monitoring of P(3HB) produced from used cooking oil with near-infrared spectroscopy

Cited by 45 publications

References 26 publications

A Process Engineering Approach to Improve Production of P(3HB) byCupriavidus necatorfrom Used Cooking Oil

A Process Engineering Approach to Improve Production of P(3HB) byCupriavidus necatorfrom Used Cooking Oil

Exploring the capabilities of fluorometric online monitoring on chinese hamster ovary cell cultivations producing a monoclonal antibody

Exploiting near infrared spectroscopy as an analytical tool for on-line monitoring of acidity during coffee roasting

Contact Info

Product

Resources

About