Online LC-MS-MS process monitoring for optimization of biological treatment of wastewater containing azo dye concentrates

Abstract

A biological high-performance treatment process comprising two 40-L reactor compartments has been developed for purification and decoloration of concentrated textile wastewater containing up to 15 g L(-1) reactive dyestuff. The decoloration rate of 95% meets the requirements of German legislation for textile wastewater treatment. Successful process development and optimization was achieved by use of high-performance liquid chromatography, with diode-array and electrospray tandem mass spectrometric (LC-ESI-MS-MS) detection, coupled with inline microfiltration membrane-sampling devices, applied online to bioreactors as a process analytical tool for the first time. The optimum process performance was found by correlation of dye and decoloration product-specific concentrations with summary properties such as redox potential and dissolved oxygen content. Details of the degradation and decoloration mechanism for the azo dye reactive black 5 was revealed by using mass spectrometry for structure elucidation.

Removal of acid orange 7 by guava seed carbon: A four parameter optimization study

“…However, COD removal was 45% at pH 7.5 and 15% at pH 2 with respect to the COD value before the adsorption experiment. The decolorization rate of >95% meets the requirements of several legislations for textile wastewater [77].…”

“…Real dyeing wastewater is a complicated matrix. Its COD values can vary between 196 mg L −1 and 894 mg L −1 [73,74] in raw samples, between 230 mg L −1 [75] and 455 mg L −1 [76] after biological treatment and contain up to 15 g L −1 of a reactive dye [77]. The last objective of our work was to test the decolorization of a real textile wastewater under the optimum removal conditions found in the previous sections-pH: 2, temperature: 15 • C, S esp : 600 m 2 g −1 and dosage 30 mg ml −1 .…”

Response surface optimization of acid red 119 dye from simulated wastewater using Al based waterworks sludge and polyaluminium chloride as coagulant

“…The decolorization rate of >95% meets the requirements of several legislations for textile wastewater (Rehorek and Plum, 2006). Therefore, in order to acquire optimal regions for AR119 dye removal using PACS and PAC, response optimizer searched for a combination of factor levels that satisfy minimum dye removal efficiency of 95%.…”

On-line high performance liquid chromatography measurements of extracellular metabolites in an aerobic batch yeast (Saccharomyces cerevisiae) culture

“…The cross-flow filtration approach we chose in this study is a rather common cell separation approach but a somewhat more special variation is agitated cross-flow filtration [22]. Coupling HPLC with mass spectrometric detection further improves the versatility of the on-line analysis for on-line applications [23,24].…”