Online extraction–high performance liquid chromatography–diode array detector–quadrupole time-of-flight tandem mass spectrometry for rapid flavonoid profiling of Fructus aurantii immaturus

Tong, Runna; Peng, Mijun; Tong, Chaoying; Guo, Keke; Shi, Shuyun

doi:10.1016/j.jchromb.2018.01.031

Cited by 23 publications

(4 citation statements)

References 28 publications

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“…For the ethanolic extract of P. arboreum leaves, the HPLC profile demonstrated in the chromatogram at 330 nm was as follows: (i) high polarity peaks were observed at Rt from 1.83 to 2.16 min with absorptions at 276 and 290 nm, respectively, which could be attributed to the presence of amides; these results are consistent with those of Nascimento et al . (2015) and Silva Costa (2002); and (ii) medium polarity peaks at Rt of 13.1, 14.2 and 24.4 min with absorption bands at 268, 338, 269, 338 nm, suggestive of flavonoids (Tong et al ., 2018) and at 324 nm that may be characteristic of phenolic compounds (Fig. 8B) (Ferreres et al ., 2014).…”

Section: Resultsmentioning

confidence: 98%

Antiparasitic effects of ethanolic extracts of Piper arboreum and Jatropha gossypiifolia leaves on cercariae and adult worms of Schistosoma mansoni

et al. 2020

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New treatment strategies for schistosomiasis should be evaluated, since resistant strains to the only available drug, Praziquantel, have already been described. Thus, we demonstrated antiparasitic effects of ethanolic extracts of Jatropha gossypiifolia and Piper arboreum on cercariae and adult worms of Schistosoma mansoni. The bioassays were performed at 0–10 000 μg mL−1 concentration for 0–72 h. Adult worms were stained with carmine to assess external and internal damage. The chemical screening was performed using high-performance liquid chromatography. P. arboreum displayed the best cercaricidal effect, with a 100% reduction in viability in just 60 min. The extract of J. gossypiifolia was more effective against adult worms, with 100% viability reduction of male and female worms after 12 and 24 h, respectively. P. arboreum and J. gossypiifolia were equally effective in inhibiting the oviposition of S. mansoni (93% reduction) and causing damage to internal and external structures in adult worms. Flavonoids were identified in both the extracts and phenolic compounds and amides only in P. arboreum. Thus, for the first time, it was proven that ethanolic extracts of P. arboreum and J. gossypiifolia leaves are biologically active against cercariae and adult worms of S. mansoni in vitro.

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Section: Resultsmentioning

confidence: 98%

Antiparasitic effects of ethanolic extracts of Piper arboreum and Jatropha gossypiifolia leaves on cercariae and adult worms of Schistosoma mansoni

et al. 2020

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“…In LC-Q-TOF-MS for the profiling analysis of phenolic antioxidants, hyphenation to DAD detectors has been commonly employed for further peak annotation or dereplication, providing valuable on-line structural information based on retention times, UV spectra, exact mass, and fragmentation ions from MS/MS spectra. On-line HPLC-DAD-Q-TOF-MS/MS system was applied for the rapid chemical profiling of flavonoids form Fructus aurantii immaturus [16], minor antioxidants from leaves of Malus doumeri [34] and unknown and minor isomeric chlorogenic acids in the buds of Lonicera macranthoides [35], whereas alternative off-line configurations (HPLC-UV/Q-TOF-MS) were proposed for the rapid screening of brainpenetrable antioxidants from natural products [18] and for active fragments-guided discovery of selective tropane alkaloids drugs [19]. Other screening methods for the determination of nitric oxide scavengers [36], and other active and functional compound combinations [37] evidenced the broad applicability of the hyphenated DAD-Q-TOF systems.…”

Section: Tof-ms Methodsmentioning

confidence: 99%

“…Thus, the selection of the most appropriate ionization method according to the polarity and molecular weight of target metabolites plays an important role on their detectability. A broad variety of secondary metabolites in plant natural products, including phenolics, flavonoids, terpenoids, alkaloids, coumarin, and saponins, among others, have been analyzed using ESI-related ambient ionization techniques coupled to different HRMS instruments such as TOF [11][12][13] , Q-TOF [14][15][16][17][18][19][20][21], IT-TOF [22][23][24], LTQ-Orbitrap [25][26][27], Q-Orbitrap or Fourier-transform ion cyclotron resonance (FT-ICR) [28].…”

Section: Ionization Techniquesmentioning

confidence: 99%

Recent applications of high resolution mass spectrometry for the characterization of plant natural products

Álvarez-Rivera

Ballesteros-Vivas

Parada-Alfonso

et al. 2019

TrAC Trends in Analytical Chemistry

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Recently, the high analytical power provided by the new HRMS instruments (working in the MS or MS/MS mode, stand alone or hyphenated with separation techniques) is making more feasible the characterization of plant secondary metabolites. In this regard, HRMS-based techniques are becoming the technique of choice, since they are capable of answering many questions regarding analytical characterization of secondary metabolites, usually found in very complex matrices, including their structure, composition and concentration in a very fast and sensitive way. In this manuscript we will provide a critical and updated revision of this topic covering the works published in the last 4 years (2015)(2016)(2017)(2018), including the different configurations in which HRMS may be used (stand alone or hyphenated), the data treatment, the strategies for the identification of unknown metabolites, the current software for structure elucidation, as well as the last trends and future outlooks in this hot area of research.

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“…Due to their specific therapeutic effects, four decoction pieces are employed to perform diverse usages. AFI is often used to eliminate phlegm and dissipate stagnant qi, while AF relieves gastrointestinal indigestion in a gentle yet effective manner [ 3 , 4 ]. CRPV is mostly used to promote the flow of liver qi, while CRP is commonly utilized to strengthen the spleen and dispel phlegm [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Study on Closely Related Citrus CMMs based on Chemometrics and Prediction of Components-Targets-Diseases Network by Ingenuity Pathway Analysis

Zhang

Cui

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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As the representatives of closely related Chinese medicinal materials (CMMs) originated from Rutaceae family, Aurantii fructus immaturus (AFI), Aurantii fructus (AF), Citri reticulatae pericarpium viride (CRPV), and Citri reticulatae pericarpium (CRP) have better functions in regulating qi and promoting gastrointestinal motility. However, differences in the quality of closely related Citrus CMMs have not yet been revealed until now. Herein, this study focused on the systematic differentiation and in-depth understanding of closely related Citrus CMMs by a strategy integrating chemometrics and network pharmacology. Determined by ultra performance liquid chromatography, the content of nine flavonoids showed obvious fluctuations in the decoction pieces from different species (Citrus aurantium Linnaeus and Citrus reticulate Blanco) with decreasing levels in the samples of ripe fruits. Decoction pieces from the different species and ripening stages were well distinguished by orthogonal projection to latent structure-discriminate analysis (OPLS-DA) and cluster analysis. As a result, four active components including narirutin, naringenin, hesperidin, and 3,5,6,7,8,3′,4′-heptemthoxyflavone were filtered out by variable importance for the projection (VIP) value (VIP > 1.0), which were regarded as chemotaxonomic markers. Furthermore, a components-targets-diseases network was constructed via ingenuity pathway analysis (IPA), and the correlations were predicted between four chemotaxonomic markers, 223 targets, and three diseases including colitis, breast cancer, and colorectal cancer. The obtained results will be of great significance for identifying closely related Citrus CMMs and conduce to improving the resource utilization of CMMs.

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Online extraction–high performance liquid chromatography–diode array detector–quadrupole time-of-flight tandem mass spectrometry for rapid flavonoid profiling of Fructus aurantii immaturus

Cited by 23 publications

References 28 publications

Antiparasitic effects of ethanolic extracts of Piper arboreum and Jatropha gossypiifolia leaves on cercariae and adult worms of Schistosoma mansoni

Antiparasitic effects of ethanolic extracts of Piper arboreum and Jatropha gossypiifolia leaves on cercariae and adult worms of Schistosoma mansoni

Recent applications of high resolution mass spectrometry for the characterization of plant natural products

Study on Closely Related Citrus CMMs based on Chemometrics and Prediction of Components-Targets-Diseases Network by Ingenuity Pathway Analysis

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